Serum miR-30c-5p is a potential biomarker for multiple system atrophy.
| Title: | Serum miR-30c-5p is a potential biomarker for multiple system atrophy. |
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| Authors: | Vallelunga A; Neuroscience Section, Department of Medicine, Surgery and Dentistry 'Scuola Medica Salernitana', University of Salerno, Salerno, Italy. vallelungaannamaria@gmail.com.; Iannitti T; KWS BioTest, Marine View Office Park, Portishead, Somerset, BS20 7AW, UK.; Dati G; Neuroscience Section, Department of Medicine, Surgery and Dentistry 'Scuola Medica Salernitana', University of Salerno, Salerno, Italy.; Capece S; Neuroscience Section, Department of Medicine, Surgery and Dentistry 'Scuola Medica Salernitana', University of Salerno, Salerno, Italy.; Maugeri M; Department of Rheumatology and Inflammation Research, Sahlgrenska Academy, University of Gothenburg, Gothenburg, Sweden.; Tocci E; Laboratorio Analisi PO Serra San Bruno, ASP Vibo Valentia, Vibo Valentia, Italy.; Picillo M; Neuroscience Section, Department of Medicine, Surgery and Dentistry 'Scuola Medica Salernitana', University of Salerno, Salerno, Italy.; Volpe G; Clinica Neurologica, AOU San Giovanni di Dio e Ruggi d'Aragona, Salerno, Italy.; Cozzolino A; AO San Giuseppe Moscati, Avellino, Italy.; Squillante M; Clinica Neurologica, AOU San Giovanni di Dio e Ruggi d'Aragona, Salerno, Italy.; Cicarelli G; AO San Giuseppe Moscati, Avellino, Italy.; Barone P; Neuroscience Section, Department of Medicine, Surgery and Dentistry 'Scuola Medica Salernitana', University of Salerno, Salerno, Italy.; Pellecchia MT; Neuroscience Section, Department of Medicine, Surgery and Dentistry 'Scuola Medica Salernitana', University of Salerno, Salerno, Italy. |
| Source: | Molecular biology reports [Mol Biol Rep] 2019 Apr; Vol. 46 (2), pp. 1661-1666. Date of Electronic Publication: 2019 Feb 27. |
| Publication Type: | Journal Article |
| Language: | English |
| Journal Info: | Publisher: Reidel Country of Publication: Netherlands NLM ID: 0403234 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1573-4978 (Electronic) Linking ISSN: 03014851 NLM ISO Abbreviation: Mol Biol Rep Subsets: MEDLINE |
| Imprint Name(s): | Original Publication: Dordrecht, Boston, Reidel. |
| MeSH Terms: | MicroRNAs/*blood ; Multiple System Atrophy/*blood ; Multiple System Atrophy/*genetics; Biomarkers/blood ; Gene Expression Profiling/methods ; MicroRNAs/genetics ; Multiple System Atrophy/diagnosis ; Parkinson Disease/blood ; Parkinson Disease/genetics ; Aged ; Disease Progression ; Female ; Gene Expression Regulation ; Humans ; Male ; Middle Aged ; Real-Time Polymerase Chain Reaction ; Transcriptome ; Up-Regulation |
| Abstract: | Multiple system atrophy (MSA) is a neurodegenerative disease that belongs to the α synucleinopathies. Clinically, there is an overlap between MSA and Parkinson's disease (PD), especially at the early disease stage. However, these two pathologies differ in terms of disease progression. Currently, no biomarker exists to differentiate MSA from PD. MicroRNAs are non-coding RNAs implicated in gene expression regulation. MiRNAs modulate cellular activity and they control a range of physiological and pathological functions. miRNAs are found in biofluids, such as blood, serum, plasma, saliva, and cerebrospinal fluid. Many groups, including ours, found that circulating miRNAs are differently expressed in blood, plasma, serum and cerebrospinal fluid of PD and MSA patients. In the present study, our primary aim was to determine if serum mir-30-5p and mir-148b-5p can be used as biomarkers for early diagnosis of PD and/or MSA. Our secondary goal was to determine if serum levels of those miRNAs can be correlated with the patients' clinical profile. Using quantitative PCR (qPCR), we evaluated expression levels of miR-30c-5p and miR148b-5p in serum samples from PD (n = 56), MSA (n = 49), and healthy control (n = 50) subjects. We have found that miR-30c-5p is significantly upregulated in MSA if compared with PD and healthy control subjects. Moreover, serum miR-30c-5p levels correlate with disease duration in both MSA and PD. No significant difference was found in miR-148b-5p among MSA, PD and healthy control subjects. Our results suggest a possible role of serum miR-30-5p as a biomarker for diagnosis and progression of MSA. |
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| Contributed Indexing: | Keywords: Biomarker; MiR-30c-5p; MiRNAs; Multiple system atrophy; Parkinson’s disease; Synucleinopathies |
| Substance Nomenclature: | 0 (Biomarkers); 0 (MIRN148 microRNA, human); 0 (MIRN30b microRNA, human); 0 (MicroRNAs) |
| Entry Date(s): | Date Created: 20190228 Date Completed: 20190909 Latest Revision: 20210719 |
| Update Code: | 20260130 |
| DOI: | 10.1007/s11033-019-04614-z |
| PMID: | 30810945 |
| Database: | MEDLINE |
Journal Article