Contrasting Pathways for Anaerobic Methane Oxidation in Gulf of Mexico Cold Seep Sediments.
| Title: | Contrasting Pathways for Anaerobic Methane Oxidation in Gulf of Mexico Cold Seep Sediments. |
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| Authors: | Vigneron A; School of Natural and Environmental Sciences, Newcastle University, Newcastle Upon Tyne, United Kingdom.; Shell International Exploration and Production Inc., Houston, Texas, USA.; Alsop EB; Shell International Exploration and Production Inc., Houston, Texas, USA.; DOE Joint Genome Institute, Walnut Creek, California, USA.; Cruaud P; Departement de Biochimie, de Microbiologie, et de Bio-Informatique, Faculté des Sciences et de Génie, Université Laval, Quebec City, Quebec, Canada.; Philibert G; Shell International Exploration and Production Inc., Houston, Texas, USA.; King B; Shell International Exploration and Production Inc., Houston, Texas, USA.; Baksmaty L; Shell International Exploration and Production Inc., Houston, Texas, USA.; Lavallee D; Shell International Exploration and Production Inc., Houston, Texas, USA.; Lomans BP; Shell Global Solutions International B.V., Rijswijk, Netherlands.; Eloe-Fadrosh E; DOE Joint Genome Institute, Walnut Creek, California, USA.; Kyrpides NC; DOE Joint Genome Institute, Walnut Creek, California, USA.; Head IM; School of Natural and Environmental Sciences, Newcastle University, Newcastle Upon Tyne, United Kingdom.; Tsesmetzis N; School of Natural and Environmental Sciences, Newcastle University, Newcastle Upon Tyne, United Kingdom.; Shell International Exploration and Production Inc., Houston, Texas, USA. |
| Source: | MSystems [mSystems] 2019 Feb 26; Vol. 4 (1). Date of Electronic Publication: 2019 Feb 26 (Print Publication: 2019). |
| Publication Type: | Journal Article |
| Language: | English |
| Journal Info: | Publisher: American Society for Microbiology Country of Publication: United States NLM ID: 101680636 Publication Model: eCollection Cited Medium: Print ISSN: 2379-5077 (Print) Linking ISSN: 23795077 NLM ISO Abbreviation: mSystems Subsets: PubMed not MEDLINE |
| Imprint Name(s): | Original Publication: Washington, DC : American Society for Microbiology, [2016]- |
| Abstract: | Gulf of Mexico sediments harbor numerous hydrocarbon seeps associated with high sedimentation rates and thermal maturation of organic matter. These ecosystems host abundant and diverse microbial communities that directly or indirectly metabolize components of the emitted fluid. To investigate microbial function and activities in these ecosystems, metabolic potential (metagenomic) and gene expression (metatranscriptomic) analyses of two cold seep areas of the Gulf of Mexico were carried out. Seeps emitting biogenic methane harbored microbial communities dominated by archaeal anaerobic methane oxidizers of phylogenetic group 1 (ANME-1), whereas seeps producing fluids containing a complex mixture of thermogenic hydrocarbons were dominated by ANME-2 lineages. Metatranscriptome measurements in both communities indicated high levels of expression of genes for methane metabolism despite their distinct microbial communities and hydrocarbon composition. In contrast, the transcription level of sulfur cycle genes was quite different. In the thermogenic seep community, high levels of transcripts indicative of syntrophic anaerobic oxidation of methane (AOM) coupled to sulfate reduction were detected. This syntrophic partnership between the dominant ANME-2 and sulfate reducers potentially involves direct electron transfer through multiheme cytochromes. In the biogenic methane seep, genes from an ANME-1 lineage that are potentially involved in polysulfide reduction were highly expressed, suggesting a novel bacterium-independent anaerobic methane oxidation pathway coupled to polysulfide reduction. The observed divergence in AOM activities provides a new model for bacterium-independent AOM and emphasizes the variation that exists in AOM pathways between different ANME lineages. IMPORTANCE Cold seep sediments are complex and widespread marine ecosystems emitting large amounts of methane, a potent greenhouse gas, and other hydrocarbons. Within these sediments, microbial communities play crucial roles in production and degradation of hydrocarbons, modulating oil and gas emissions to seawater. Despite this ecological importance, our understanding of microbial functions and methane oxidation pathways in cold seep ecosystems is poor. Based on gene expression profiling of environmental seep sediment samples, the present work showed that (i) the composition of the emitted fluids shapes the microbial community in general and the anaerobic methanotroph community specifically and (ii) AOM by ANME-2 in this seep may be coupled to sulfate reduction by Deltaproteobacteria by electron transfer through multiheme cytochromes, whereas AOM by ANME-1 lineages in this seep may involve a different, bacterium-independent pathway, coupling methane oxidation to elemental sulfur/polysulfide reduction. |
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| Contributed Indexing: | Keywords: AOM; metagenomic; metatranscriptomic; methane; polysulfide |
| Entry Date(s): | Date Created: 20190306 Latest Revision: 20201001 |
| Update Code: | 20260130 |
| PubMed Central ID: | PMC6392090 |
| DOI: | 10.1128/mSystems.00091-18 |
| PMID: | 30834326 |
| Database: | MEDLINE |
Journal Article