Comparison of the Accuracy of Four Malaria Diagnostic Methods in a High Transmission Setting in Coastal Cameroon.
| Title: | Comparison of the Accuracy of Four Malaria Diagnostic Methods in a High Transmission Setting in Coastal Cameroon. |
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| Authors: | Moyeh MN; Department of Biochemistry and Molecular Biology, University of Buea, Buea, Cameroon.; The Biotechnology Centre, University of Yaoundé 1, Yaoundé, Cameroon.; The Biotechnology Unit, University of Buea, Cameroon.; Ali IM; The Biotechnology Centre, University of Yaoundé 1, Yaoundé, Cameroon.; Department of Biochemistry, University of Dschang, Dschang, Cameroon.; Njimoh DL; Department of Biochemistry and Molecular Biology, University of Buea, Buea, Cameroon.; The Biotechnology Unit, University of Buea, Cameroon.; Nji AM; The Biotechnology Centre, University of Yaoundé 1, Yaoundé, Cameroon.; Department of Biochemistry, University of Yaoundé 1, Yaoundé, Cameroon.; Netongo PM; The Biotechnology Centre, University of Yaoundé 1, Yaoundé, Cameroon.; Department of Biochemistry, University of Yaoundé 1, Yaoundé, Cameroon.; Evehe MS; The Biotechnology Centre, University of Yaoundé 1, Yaoundé, Cameroon.; Department of Biochemistry, University of Yaoundé 1, Yaoundé, Cameroon.; Atogho-Tiedeu B; The Biotechnology Centre, University of Yaoundé 1, Yaoundé, Cameroon.; Department of Biochemistry, University of Yaoundé 1, Yaoundé, Cameroon.; Ghogomu SM; Department of Biochemistry and Molecular Biology, University of Buea, Buea, Cameroon.; The Biotechnology Unit, University of Buea, Cameroon.; Mbacham WF; The Biotechnology Centre, University of Yaoundé 1, Yaoundé, Cameroon.; Department of Biochemistry, University of Yaoundé 1, Yaoundé, Cameroon. |
| Source: | Journal of parasitology research [J Parasitol Res] 2019 Mar 10; Vol. 2019, pp. 1417967. Date of Electronic Publication: 2019 Mar 10 (Print Publication: 2019). |
| Publication Type: | Journal Article |
| Language: | English |
| Journal Info: | Publisher: Wiley Country of Publication: United States NLM ID: 101526294 Publication Model: eCollection Cited Medium: Print ISSN: 2090-0023 (Print) Linking ISSN: 20900023 NLM ISO Abbreviation: J Parasitol Res Subsets: PubMed not MEDLINE |
| Imprint Name(s): | Publication: 2023- : [Hoboken, NJ] : Wiley; Original Publication: New York : Hindawi Pub. Corp., 2009- |
| Abstract: | Background: Despite recommendation from the World Health Organization that all malaria suspected patients undergo a parasitological confirmation using rapid diagnostic test or light microscopy prior to treatment, health facilities in remote malaria endemic settings sometimes resort to presumptive diagnosis of malaria for clinical management for various reasons. Following observation of this practice, we undertook a cross-sectional study aimed at comparing presumptive diagnosis based on axillary temperature, SD Bioline™ rapid test, and light microscopy as strategies for malaria diagnosis in the coastal region of Mutengene in the South West of Cameroon with the overall goal of supporting improved malaria diagnosis at local levels.; Methodology: Venous blood from 320 participants was used to detect the presence of malaria parasite using SD Bioline™ mRDT and Giemsa stained microscopy or spotted on filter paper for PCR amplification of the 18s rRNA gene of Plasmodium sp following standard procedures. The axillary temperature of each participant was also measured. The sensitivity, specificity, and predictive values and their confidence intervals were determined for each of the methods with PCR as the reference. The area under the curve was used to estimate accuracy of diagnostic method and compared between test method using the X2 test with P |
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| Grant Information: | United Kingdom WT_ Wellcome Trust; EP-C-15-010 United States EPA EPA |
| Entry Date(s): | Date Created: 20190416 Latest Revision: 20260303 |
| Update Code: | 20260303 |
| PubMed Central ID: | PMC6431520 |
| DOI: | 10.1155/2019/1417967 |
| PMID: | 30984417 |
| Database: | MEDLINE |
Journal Article