Xenotransplantation of human dental pulp stem cells in platelet-rich plasma for the treatment of full-thickness articular cartilage defects in a rabbit model.
| Title: | Xenotransplantation of human dental pulp stem cells in platelet-rich plasma for the treatment of full-thickness articular cartilage defects in a rabbit model. |
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| Authors: | Yanasse RH; Department of Genetics, Blood Center, Faculdade de Medicina de Marília (FAMEMA), Marília, SP 17519-050, Brazil.; De Lábio RW; Department of Genetics, Blood Center, Faculdade de Medicina de Marília (FAMEMA), Marília, SP 17519-050, Brazil.; Marques L; Department of Health Sciences, Universidade do Sagrado Coração, Bauru, SP 17519-050, Brazil.; Fukasawa JT; Department of Genetics, Blood Center, Faculdade de Medicina de Marília (FAMEMA), Marília, SP 17519-050, Brazil.; Segato R; Department of Genetics, Blood Center, Faculdade de Medicina de Marília (FAMEMA), Marília, SP 17519-050, Brazil.; Kinoshita A; Department of Health Sciences, Universidade do Sagrado Coração, Bauru, SP 17519-050, Brazil.; Matsumoto MA; Department of Health Sciences, Universidade do Sagrado Coração, Bauru, SP 17519-050, Brazil.; Felisbino SL; Department of Morphology, Universidade Estadual Paulista Júlio de Mesquita Filho (UNESP), Botucatu, SP 17519-050, Brazil.; Solano B; Center for Biotechnology and Cell Therapy, Monte Tabor Hospital São Rafael, Salvador, BA 17519-050, Brazil.; Dos Santos RR; Center for Biotechnology and Cell Therapy, Monte Tabor Hospital São Rafael, Salvador, BA 17519-050, Brazil.; Payão SLM; Department of Genetics, Blood Center, Faculdade de Medicina de Marília (FAMEMA), Marília, SP 17519-050, Brazil.; Department of Health Sciences, Universidade do Sagrado Coração, Bauru, SP 17519-050, Brazil. |
| Source: | Experimental and therapeutic medicine [Exp Ther Med] 2019 Jun; Vol. 17 (6), pp. 4344-4356. Date of Electronic Publication: 2019 Apr 17. |
| Publication Type: | Journal Article |
| Language: | English |
| Journal Info: | Publisher: Spandidos Pub.] Country of Publication: Greece NLM ID: 101531947 Publication Model: Print-Electronic Cited Medium: Print ISSN: 1792-0981 (Print) Linking ISSN: 17920981 NLM ISO Abbreviation: Exp Ther Med Subsets: PubMed not MEDLINE |
| Imprint Name(s): | Original Publication: [Athens : Spandidos Pub.] |
| Abstract: | Stem cells in platelet-rich plasma (PRP) scaffolds may be a promising treatment for cartilage repair. Human dental pulp stem cell (hDPSC) subpopulations have been identified to have substantial angiogenic, neurogenic and regenerative potential when compared with other stem cell sources. The present study evaluated the potential of hDPSCs in a PRP scaffold to regenerate full-thickness cartilage defects in rabbits. Full-thickness articular cartilage defects were created in the patellar groove of the femur of 30 rabbits allocated into three experimental groups: Those with an untreated critical defect (CTL), those treated with PRP (PRP) and those treated with stem cells in a PRP scaffold (PRP+SC). The patellar grooves of the femurs from the experimental groups were evaluated macroscopically and histologically at 6 and 12 weeks post-surgery. The synovial membranes were also collected and evaluated for histopathological analysis. The synovial lining cell layer was enlarged in the CTL group compared with the PRP group at 6 weeks (P=0.037) but not with the PRP+SC group. All groups exhibited low-grade synovitis at 6 weeks and no synovitis at 12 weeks. Notably, macroscopic grades for the area of articular cartilage repair for the PRP+SC group were significantly improved compared with those in the CTL (P=0.001) and PRP (P=0.049) groups at 12 weeks. Furthermore, histological scores (modified O'Driscoll scoring system) of the patellar groove articular cartilage in the PRP+SC and PRP groups, in which the articular cartilage was primarily hyaline-like, were significantly higher compared with those in the CTL group at 12 weeks (P=0.002 and P=0.007, respectively). The present results support the therapeutic use of hDPSCs for the treatment of full-thickness articular cartilage defects. |
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| Contributed Indexing: | Keywords: cartilage; dental pulp; knee; platelet-rich plasma; stem cells |
| Entry Date(s): | Date Created: 20190613 Latest Revision: 20201001 |
| Update Code: | 20260130 |
| PubMed Central ID: | PMC6507499 |
| DOI: | 10.3892/etm.2019.7499 |
| PMID: | 31186677 |
| Database: | MEDLINE |
Journal Article