Identification of microRNAS differentially regulated by water deficit in relation to mycorrhizal treatment in wheat.
| Title: | Identification of microRNAS differentially regulated by water deficit in relation to mycorrhizal treatment in wheat. |
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| Authors: | Fileccia V; Dipartimento di Scienze Agrarie, Alimentari e Forestali, Università degli Studi di Palermo, Palermo, Italy.; Ingraffia R; Dipartimento di Scienze Agrarie, Alimentari e Forestali, Università degli Studi di Palermo, Palermo, Italy.; Amato G; Dipartimento di Scienze Agrarie, Alimentari e Forestali, Università degli Studi di Palermo, Palermo, Italy.; Giambalvo D; Dipartimento di Scienze Agrarie, Alimentari e Forestali, Università degli Studi di Palermo, Palermo, Italy.; Martinelli F; Department of Biology, University of Florence, Sesto Fiorentino, Italy. federico.martinelli@unifi.it. |
| Source: | Molecular biology reports [Mol Biol Rep] 2019 Oct; Vol. 46 (5), pp. 5163-5174. Date of Electronic Publication: 2019 Jul 20. |
| Publication Type: | Journal Article |
| Language: | English |
| Journal Info: | Publisher: Reidel Country of Publication: Netherlands NLM ID: 0403234 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1573-4978 (Electronic) Linking ISSN: 03014851 NLM ISO Abbreviation: Mol Biol Rep Subsets: MEDLINE |
| Imprint Name(s): | Original Publication: Dordrecht, Boston, Reidel. |
| MeSH Terms: | Gene Regulatory Networks*; MicroRNAs/*genetics ; Mycorrhizae/*physiology ; Triticum/*growth & development; Gene Expression Profiling/methods ; Plant Leaves/drug effects ; Plant Leaves/genetics ; Plant Leaves/growth & development ; Plant Proteins/genetics ; Plant Roots/drug effects ; Plant Roots/genetics ; Plant Roots/growth & development ; RNA, Plant/genetics ; Triticum/genetics ; Triticum/microbiology ; Droughts ; Gene Expression Regulation, Developmental ; Gene Expression Regulation, Plant ; Stress, Physiological |
| Abstract: | Arbuscular mycorrhizal fungi (AMF) are soil microrganisms that establish symbiosis with plants positively influencing their resistance to abiotic stresses. The aim of this work was to identify wheat miRNAs differentially regulated by water deficit conditions in presence or absence of AMF treatment. Small RNA libraries were constructed for both leaf and root tissues considering four conditions: control (irrigated) or water deficit in presence/absence of mycorrhizal (AMF) treatment. A total of 12 miRNAs were significantly regulated by water deficit in leaves: five in absence and seven in presence of AMF treatment. In roots, three miRNAs were water deficit-modulated in absence of mycorrhizal treatment while six were regulated in presence of it. The most represented miRNA family was miR167 that was regulated by water deficit in both leaf and root tissues. Interestingly, miR827-5p was differentially regulated in leaves in the absence of mycorrhizal treatment while it was water deficit-modulated in roots irrespective of AMF treatment. In roots, water deficit repressed miR827-5p, miR394, miR6187, miR167e-3p, and miR9666b-3p affecting transcription, RNA synthesis, protein synthesis, and protein modifications. In leaves, mycorrhizae modulated miR5384-3p and miR156e-3p affecting trafficking and cell redox homeostasis. DNA replication and transcription regulation should be targeted by the repression of miR1432-5p and miR166h-3p. This work provided interesting insights into the post-transcriptional mechanisms of wheat responses to water deficit in relation to mycorrhizal symbiosis. |
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| Contributed Indexing: | Keywords: Durum wheat; Mycorrhizae; Roots; Water deficit; Wheat; miRNA |
| Substance Nomenclature: | 0 (MicroRNAs); 0 (Plant Proteins); 0 (RNA, Plant) |
| Entry Date(s): | Date Created: 20190722 Date Completed: 20200217 Latest Revision: 20220125 |
| Update Code: | 20260130 |
| DOI: | 10.1007/s11033-019-04974-6 |
| PMID: | 31327121 |
| Database: | MEDLINE |
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