Mechanical Stabilization of the Glandular Acinus by Linker of Nucleoskeleton and Cytoskeleton Complex.
| Title: | Mechanical Stabilization of the Glandular Acinus by Linker of Nucleoskeleton and Cytoskeleton Complex. |
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| Authors: | Zhang Q; Department of Chemical Engineering, University of Florida, Gainesville, FL 32611, USA.; Narayanan V; Department of Biomedical Engineering, Virginia Commonwealth University, Richmond, VA 23284, USA.; Mui KL; Department of Pathology and Cell Biology, Vagelos College of Physicians and Surgeons, Columbia University, New York, NY 10032, USA.; O'Bryan CS; Department of Mechanical and Aerospace Engineering, University of Florida, Gainesville, FL 32611, USA.; Anderson RH; Enabling Technologies Group, Sanford Research, Sioux Falls, SD 57104, USA.; Kc B; Enabling Technologies Group, Sanford Research, Sioux Falls, SD 57104, USA.; Cabe JI; Department of Biomedical Engineering, Virginia Commonwealth University, Richmond, VA 23284, USA.; Denis KB; Department of Biomedical Engineering, Virginia Commonwealth University, Richmond, VA 23284, USA.; Antoku S; Department of Pathology and Cell Biology, Vagelos College of Physicians and Surgeons, Columbia University, New York, NY 10032, USA.; Roux KJ; Enabling Technologies Group, Sanford Research, Sioux Falls, SD 57104, USA; Department of Pediatrics, Sanford School of Medicine, University of South Dakota, Sioux Falls, SD 57105, USA.; Dickinson RB; Department of Chemical Engineering, University of Florida, Gainesville, FL 32611, USA.; Angelini TE; Department of Mechanical and Aerospace Engineering, University of Florida, Gainesville, FL 32611, USA.; Gundersen GG; Department of Pathology and Cell Biology, Vagelos College of Physicians and Surgeons, Columbia University, New York, NY 10032, USA.; Conway DE; Department of Biomedical Engineering, Virginia Commonwealth University, Richmond, VA 23284, USA. Electronic address: dconway@vcu.edu.; Lele TP; Department of Chemical Engineering, University of Florida, Gainesville, FL 32611, USA. Electronic address: tlele@che.ufl.edu. |
| Source: | Current biology : CB [Curr Biol] 2019 Sep 09; Vol. 29 (17), pp. 2826-2839.e4. Date of Electronic Publication: 2019 Aug 08. |
| Publication Type: | Journal Article; Research Support, N.I.H., Extramural; Research Support, U.S. Gov't, Non-P.H.S. |
| Language: | English |
| Journal Info: | Publisher: Cell Press Country of Publication: England NLM ID: 9107782 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1879-0445 (Electronic) Linking ISSN: 09609822 NLM ISO Abbreviation: Curr Biol Subsets: MEDLINE |
| Imprint Name(s): | Publication: Cambridge, MA : Cell Press; Original Publication: London, UK : Current Biology Ltd., c1991- |
| MeSH Terms: | Acinar Cells/*physiology ; Cytoskeleton/*physiology ; Nuclear Matrix/*physiology; Animals ; Biomechanical Phenomena ; Dogs ; Humans ; Madin Darby Canine Kidney Cells |
| Abstract: | The nucleoskeleton and cytoskeleton are important protein networks that govern cellular behavior and are connected together by the linker of nucleoskeleton and cytoskeleton (LINC) complex. Mutations in LINC complex components may be relevant to cancer, but how cell-level changes might translate into tissue-level malignancy is unclear. We used glandular epithelial cells in a three-dimensional culture model to investigate the effect of perturbations of the LINC complex on higher order cellular architecture. We show that inducible LINC complex disruption in human mammary epithelial MCF-10A cells and canine kidney epithelial MDCK II cells mechanically destabilizes the acinus. Lumenal collapse occurs because the acinus is unstable to increased mechanical tension that is caused by upregulation of Rho-kinase-dependent non-muscle myosin II motor activity. These findings provide a potential mechanistic explanation for how disruption of LINC complex may contribute to a loss of tissue structure in glandular epithelia.; (Copyright © 2019 Elsevier Ltd. All rights reserved.) |
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| Grant Information: | F32 CA221320 United States CA NCI NIH HHS; R01 EB014869 United States EB NIBIB NIH HHS; P20 GM103620 United States GM NIGMS NIH HHS; R35 GM119617 United States GM NIGMS NIH HHS; R35 GM126949 United States GM NIGMS NIH HHS; R01 GM099481 United States GM NIGMS NIH HHS; P30 CA016059 United States CA NCI NIH HHS; P20 GM103548 United States GM NIGMS NIH HHS |
| Contributed Indexing: | Keywords: 3D culture; LINC complex; acinar development; actomyosin contractility; breast cancer; fluctuations; lumenal filling; nuclear mechanics; nucleus; tissue mechanics |
| Entry Date(s): | Date Created: 20190813 Date Completed: 20200730 Latest Revision: 20231219 |
| Update Code: | 20260130 |
| PubMed Central ID: | PMC6736724 |
| DOI: | 10.1016/j.cub.2019.07.021 |
| PMID: | 31402305 |
| Database: | MEDLINE |
Journal Article; Research Support, N.I.H., Extramural; Research Support, U.S. Gov't, Non-P.H.S.