Dealing with biofilms of Pseudomonas aeruginosa and Staphylococcus aureus: In vitro evaluation of a novel aerosol formulation of silver sulfadiazine.
| Title: | Dealing with biofilms of Pseudomonas aeruginosa and Staphylococcus aureus: In vitro evaluation of a novel aerosol formulation of silver sulfadiazine. |
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| Authors: | Moyano AJ; Universidad Nacional de Córdoba, Facultad de Ciencias Químicas, Departamento de Química Biológica Ranwel Caputto, Córdoba, Argentina; CONICET, Centro de Investigaciones en Química Biológica de Córdoba (CIQUIBIC), Córdoba, Argentina. Electronic address: amoyano@fcq.unc.edu.ar.; Mas CR; Universidad Nacional de Córdoba, Facultad de Ciencias Químicas, Departamento de Química Biológica Ranwel Caputto, Córdoba, Argentina; CONICET, Centro de Investigaciones en Química Biológica de Córdoba (CIQUIBIC), Córdoba, Argentina.; Colque CA; Universidad Nacional de Córdoba, Facultad de Ciencias Químicas, Departamento de Química Biológica Ranwel Caputto, Córdoba, Argentina; CONICET, Centro de Investigaciones en Química Biológica de Córdoba (CIQUIBIC), Córdoba, Argentina.; Smania AM; Universidad Nacional de Córdoba, Facultad de Ciencias Químicas, Departamento de Química Biológica Ranwel Caputto, Córdoba, Argentina; CONICET, Centro de Investigaciones en Química Biológica de Córdoba (CIQUIBIC), Córdoba, Argentina. Electronic address: asmania@fcq.unc.edu.ar. |
| Source: | Burns : journal of the International Society for Burn Injuries [Burns] 2020 Feb; Vol. 46 (1), pp. 128-135. Date of Electronic Publication: 2019 Aug 14. |
| Publication Type: | Journal Article |
| Language: | English |
| Journal Info: | Publisher: Elsevier Country of Publication: Netherlands NLM ID: 8913178 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1879-1409 (Electronic) Linking ISSN: 03054179 NLM ISO Abbreviation: Burns Subsets: MEDLINE |
| Imprint Name(s): | Publication: Amsterdam : Elsevier; Original Publication: [Guildford, Surrey, UK] : Butterworths, [c1989- |
| MeSH Terms: | Anti-Infective Agents, Local/*pharmacology ; Biofilms/*drug effects ; Methicillin-Resistant Staphylococcus aureus/*drug effects ; Microbial Viability/*drug effects ; Pseudomonas aeruginosa/*drug effects ; Silver Sulfadiazine/*pharmacology; Anesthetics, Local/administration & dosage ; Anesthetics, Local/pharmacology ; Anti-Infective Agents, Local/administration & dosage ; Lidocaine/administration & dosage ; Lidocaine/pharmacology ; Methicillin-Resistant Staphylococcus aureus/growth & development ; Methicillin-Resistant Staphylococcus aureus/metabolism ; Pseudomonas aeruginosa/growth & development ; Pseudomonas aeruginosa/metabolism ; Silver Sulfadiazine/administration & dosage ; Staphylococcus aureus/drug effects ; Staphylococcus aureus/growth & development ; Staphylococcus aureus/metabolism ; Vitamin A/administration & dosage ; Vitamin A/pharmacology ; Vitamins/administration & dosage ; Vitamins/pharmacology ; Aerosols ; Drug Combinations ; In Vitro Techniques |
| Abstract: | The risk of infection of skin and soft tissue chronic wounds by gram-negative and gram-positive pathogens growing in biofilms is a major health-care concern. In this study we test a formulation of silver sulfadiazine, vitamin A and lidocaine (AF-SSD) for aerosol administration against biofilms of Pseudomonas aeruginosa and biofilms of methicillin-resistant (MRSA) and methicillin-sensitive (MSSA) strains of Staphylococcus aureus. The aerosol allows the administration of AF-SSD without the direct contact with the wound and avoids contamination of the product after reiterative usage. We evaluated in vitro the anti-biofilm activity of AF-SSD by carrying out different technical approaches such as resazurin assays to measure metabolic activity/viability, crystal violet staining assays to determine biofilm biomass, counting of CFUs and live/dead staining for confocal microscopy analysis. AF-SSD clearly affected biofilm viability, biomass and structure, in the three bacterial strains tested. AF-SSD displayed a strong anti-biofilm effect, showing total bactericidal activity on biofilms of P. aeruginosa at a 400-fold dilution of the product, and after a 100-fold and 10-fold dilution for MRSA and MSSA, respectively. Considering the benefits of aerosol administration, our results support this kind of formulation as a potential improvement over conventional treatments with silver sulfadiazine.; (Copyright © 2019 Elsevier Ltd and ISBI. All rights reserved.) |
| Contributed Indexing: | Keywords: Pseudomonas aeruginosa; Staphylococcus aureus; biofilm; chronic wound; silver sulfadiazine |
| Substance Nomenclature: | 0 (Aerosols); 0 (Anesthetics, Local); 0 (Anti-Infective Agents, Local); 0 (Drug Combinations); 0 (Vitamins); 11103-57-4 (Vitamin A); 98PI200987 (Lidocaine); W46JY43EJR (Silver Sulfadiazine) |
| Entry Date(s): | Date Created: 20190818 Date Completed: 20201201 Latest Revision: 20201201 |
| Update Code: | 20260130 |
| DOI: | 10.1016/j.burns.2019.07.027 |
| PMID: | 31420266 |
| Database: | MEDLINE |
Journal Article