Molecular adaptation in adipose tissue in response to overfeeding with a high-fat diet under sedentary conditions in South Asian and Caucasian men.
| Title: | Molecular adaptation in adipose tissue in response to overfeeding with a high-fat diet under sedentary conditions in South Asian and Caucasian men. |
|---|---|
| Authors: | Wulan SN; Department of Nutrition and Movement Sciences, Nutrition and Toxicology Research Institute - School for Nutrition and Translational Research in Metabolism, Maastricht University Medical Center (MUMC+), Maastricht, The Netherlands.; Laboratory of Food Quality and Nutrition, Food Science and Technology Study Program, Department of Agricultural Product Technology, Faculty of Agricultural Technology, Brawijaya University, Malang-East Java, Indonesia.; Bouwman FG; Department of Human Biology, Nutrition and Toxicology Research Institute - School for Nutrition and Translational Research in Metabolism, Maastricht University Medical Center (MUMC+), Maastricht, The Netherlands.; Westerterp KR; Department of Nutrition and Movement Sciences, Nutrition and Toxicology Research Institute - School for Nutrition and Translational Research in Metabolism, Maastricht University Medical Center (MUMC+), Maastricht, The Netherlands.; Mariman ECM; Department of Human Biology, Nutrition and Toxicology Research Institute - School for Nutrition and Translational Research in Metabolism, Maastricht University Medical Center (MUMC+), Maastricht, The Netherlands.; Plasqui G; Department of Nutrition and Movement Sciences, Nutrition and Toxicology Research Institute - School for Nutrition and Translational Research in Metabolism, Maastricht University Medical Center (MUMC+), Maastricht, The Netherlands. |
| Source: | The British journal of nutrition [Br J Nutr] 2019 Aug 14; Vol. 122 (3), pp. 241-251. |
| Publication Type: | Journal Article; Research Support, Non-U.S. Gov't |
| Language: | English |
| Journal Info: | Publisher: Published on behalf of the Nutrition Society by CABI Publishing Country of Publication: England NLM ID: 0372547 Publication Model: Print Cited Medium: Internet ISSN: 1475-2662 (Electronic) Linking ISSN: 00071145 NLM ISO Abbreviation: Br J Nutr Subsets: MEDLINE |
| Imprint Name(s): | Publication: : Wallingford, Oxon, UK : Published on behalf of the Nutrition Society by CABI Publishing; Original Publication: [Cambridge, New York] Cambridge University Press. |
| MeSH Terms: | Adiposity* ; Diet, High-Fat* ; Energy Intake*; Adipose Tissue/*metabolism; Butyryl-CoA Dehydrogenase/metabolism ; Carnitine O-Palmitoyltransferase/metabolism ; Fatty Acids/metabolism ; Lipase/metabolism ; Mitochondria/metabolism ; Perilipin-1/metabolism ; Adaptation, Physiological ; Adult ; Asian People ; Biopsy ; Body Composition ; Body Mass Index ; Body Weight ; Dietary Carbohydrates ; Dietary Fats ; Dietary Proteins ; Energy Metabolism ; Exercise ; Humans ; Male ; Nutrients ; White People ; Young Adult ; Acyltransferases |
| Abstract: | For the same BMI, South Asians have a higher body fat percentage than Caucasians. There might be differences in the fatty acid (FA) handling in adipose tissue when both ethnicities are exposed to high-fat overfeeding. The objective of the present study was to investigate the molecular adaptation in relation to FA metabolism in response to overfeeding with a high-fat diet (OHFD) in South Asian and Caucasian men. Ten South Asian men (BMI 18-29 kg/m2) and ten Caucasian men (BMI 22-33 kg/m2), matched for body fat percentage, aged 20-40 years were included. A weight-maintenance diet (30 % fat, 55 % carbohydrate and 15 % protein) was given for 3 d followed by 3 d of overfeeding (150 % energy requirement) with a high-fat diet (60 % fat, 25 % carbohydrate and 15 % protein) while staying in a respiration chamber. Before and after overfeeding, abdominal subcutaneous fat biopsies were taken. Proteins were isolated, analysed and quantified for short-chain 3-hydroxyacyl-CoA dehydrogenase (HADH), carnitine palmitoyl-transferase 1α (CPT1a), adipose TAG lipase, perilipin A (PLINA), perilipin B, lipoprotein lipase and fatty acid binding protein 4 using Western blotting. OHFD decreased the HADH level (P < 0·05) in Caucasians more than in Asians (P < 0·05), but the baseline and after intervention HADH level was relatively higher in Caucasians. The level of CPT1a decreased in South Asians and increased in Caucasians (P < 0·05). PLINA did not change with diet but the level was higher in South Asians (P < 0·05). The observed differences in HADH and PLINA levels as well as in CPT1a response may be important for differences in the long-term regulation of energy (fat) metabolism in these populations. |
| Contributed Indexing: | Keywords: High-fat diet; Molecular adaptation; Overfeeding; South Asian men: Caucasian men |
| Substance Nomenclature: | EC 1.3.8.1 (Butyryl-CoA Dehydrogenase); EC 2.3.1.21 (Carnitine O-Palmitoyltransferase); 0 (Dietary Carbohydrates); 0 (Dietary Fats); 0 (Dietary Proteins); 0 (Fatty Acids); EC 3.1.1.3 (Lipase); 0 (Nutrients); 0 (Perilipin-1); EC 2.3.1.21 (CPT1A protein, human); EC 3.1.1.3 (PNPLA2 protein, human); EC 2.3.- (Acyltransferases) |
| Entry Date(s): | Date Created: 20190903 Date Completed: 20200527 Latest Revision: 20260127 |
| Update Code: | 20260130 |
| DOI: | 10.1017/S0007114519001260 |
| PMID: | 31475655 |
| Database: | MEDLINE |
Journal Article; Research Support, Non-U.S. Gov't