Shifts in the Human Gut Microbiota Structure Caused by Quadruple Helicobacter pylori Eradication Therapy.
| Title: | Shifts in the Human Gut Microbiota Structure Caused by Quadruple Helicobacter pylori Eradication Therapy. |
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| Authors: | Olekhnovich EI; Federal Research and Clinical Centre of Physical-Chemical Medicine of Federal Medical Biological Agency, Moscow, Russia.; Manolov AI; Federal Research and Clinical Centre of Physical-Chemical Medicine of Federal Medical Biological Agency, Moscow, Russia.; Samoilov AE; Federal Research and Clinical Centre of Physical-Chemical Medicine of Federal Medical Biological Agency, Moscow, Russia.; Prianichnikov NA; Federal Research and Clinical Centre of Physical-Chemical Medicine of Federal Medical Biological Agency, Moscow, Russia.; Malakhova MV; Federal Research and Clinical Centre of Physical-Chemical Medicine of Federal Medical Biological Agency, Moscow, Russia.; Tyakht AV; Federal Research and Clinical Centre of Physical-Chemical Medicine of Federal Medical Biological Agency, Moscow, Russia.; Pavlenko AV; Federal Research and Clinical Centre of Physical-Chemical Medicine of Federal Medical Biological Agency, Moscow, Russia.; Babenko VV; Federal Research and Clinical Centre of Physical-Chemical Medicine of Federal Medical Biological Agency, Moscow, Russia.; Larin AK; Federal Research and Clinical Centre of Physical-Chemical Medicine of Federal Medical Biological Agency, Moscow, Russia.; Kovarsky BA; Federal Research and Clinical Centre of Physical-Chemical Medicine of Federal Medical Biological Agency, Moscow, Russia.; Starikova EV; Federal Research and Clinical Centre of Physical-Chemical Medicine of Federal Medical Biological Agency, Moscow, Russia.; Glushchenko OE; Federal Research and Clinical Centre of Physical-Chemical Medicine of Federal Medical Biological Agency, Moscow, Russia.; Safina DD; Kazan Federal University, Kazan, Russia.; Markelova MI; Kazan Federal University, Kazan, Russia.; Boulygina EA; Kazan Federal University, Kazan, Russia.; Khusnutdinova DR; Kazan Federal University, Kazan, Russia.; Kazan Institute of Biochemistry and Biophysics, FRC Kazan Scientific Center of RAS, Kazan, Russia.; Malanin SY; Kazan Federal University, Kazan, Russia.; Abdulkhakov SR; Kazan Federal University, Kazan, Russia.; Kazan State Medical University, Kazan, Russia.; Abdulkhakov RA; Kazan State Medical University, Kazan, Russia.; Grigoryeva TV; Kazan Federal University, Kazan, Russia.; Kostryukova ES; Federal Research and Clinical Centre of Physical-Chemical Medicine of Federal Medical Biological Agency, Moscow, Russia.; Govorun VM; Federal Research and Clinical Centre of Physical-Chemical Medicine of Federal Medical Biological Agency, Moscow, Russia.; Ilina EN; Federal Research and Clinical Centre of Physical-Chemical Medicine of Federal Medical Biological Agency, Moscow, Russia. |
| Source: | Frontiers in microbiology [Front Microbiol] 2019 Aug 27; Vol. 10, pp. 1902. Date of Electronic Publication: 2019 Aug 27 (Print Publication: 2019). |
| Publication Type: | Journal Article |
| Language: | English |
| Journal Info: | Publisher: Frontiers Research Foundation Country of Publication: Switzerland NLM ID: 101548977 Publication Model: eCollection Cited Medium: Print ISSN: 1664-302X (Print) Linking ISSN: 1664302X NLM ISO Abbreviation: Front Microbiol Subsets: PubMed not MEDLINE |
| Imprint Name(s): | Original Publication: Lausanne : Frontiers Research Foundation |
| Abstract: | The human gut microbiome plays an important role both in health and disease. Use of antibiotics can alter gut microbiota composition, which can lead to various deleterious events. Here we report a whole genome sequencing metagenomic/genomic study of the intestinal microbiota changes caused by Helicobacter pylori (HP) eradication therapy. Using approaches for metagenomic data analysis we revealed a statistically significant decrease in alpha-diversity and relative abundance of Bifidobacterium adolescentis due to HP eradication therapy, while the relative abundance of Enterococcus faecium increased. We have detected changes in general metagenome resistome profiles as well: after HP eradication therapy, the ermB, CFX group, and tetQ genes were overrepresented, while tetO and tetW genes were underrepresented. We have confirmed these results with genome-resolved metagenomic approaches. MAG (metagenome-assembled genomes) abundance profiles have changed dramatically after HP eradication therapy. Focusing on ermB gene conferring resistance to macrolides, which were included in the HP eradication therapy scheme, we have shown a connection between antibiotic resistance genes (ARGs) and some overrepresented MAGs. Moreover, some E. faecium strains isolated from stool samples obtained after HP eradication have manifested greater antibiotic resistance in vitro in comparison to other isolates, as well as the higher number of ARGs conferring resistance to macrolides and tetracyclines. |
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| Contributed Indexing: | Keywords: Helicobacter pylory eradication; antibiotic resistance; enterococci; gut microbiota; horizontal gene transfer; metagenome-assembled genome |
| Entry Date(s): | Date Created: 20190912 Latest Revision: 20240719 |
| Update Code: | 20260130 |
| PubMed Central ID: | PMC6718723 |
| DOI: | 10.3389/fmicb.2019.01902 |
| PMID: | 31507546 |
| Database: | MEDLINE |
Journal Article