Interspecific molecular variation of Tunisian complex Lolium perenne L. and Festuca arundinacea Schreb. based on the internal transcribed spacer locus (ITS).
| Title: | Interspecific molecular variation of Tunisian complex Lolium perenne L. and Festuca arundinacea Schreb. based on the internal transcribed spacer locus (ITS). |
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| Authors: | Ghariani S; 1Laboratoire de Génétique Moléculaire, Immunologie et Biotechnologie, Faculté des Sciences de Tunis, Université de Tunis El Manar, 2092 Tunis, Tunisia.; Charfeddine A; 1Laboratoire de Génétique Moléculaire, Immunologie et Biotechnologie, Faculté des Sciences de Tunis, Université de Tunis El Manar, 2092 Tunis, Tunisia.; Amari M; 1Laboratoire de Génétique Moléculaire, Immunologie et Biotechnologie, Faculté des Sciences de Tunis, Université de Tunis El Manar, 2092 Tunis, Tunisia.; Chakroun M; 2Laboratoire des Productions Animales et Fourragères, Institut National de Recherche Agronomique de la Tunisie, Université de Carthage, Rue Hédi Karray, 2049 Ariana, Tunisia.; Neila TF; 1Laboratoire de Génétique Moléculaire, Immunologie et Biotechnologie, Faculté des Sciences de Tunis, Université de Tunis El Manar, 2092 Tunis, Tunisia. |
| Source: | Physiology and molecular biology of plants : an international journal of functional plant biology [Physiol Mol Biol Plants] 2020 Feb; Vol. 26 (2), pp. 331-339. Date of Electronic Publication: 2020 Jan 20. |
| Publication Type: | Journal Article |
| Language: | English |
| Journal Info: | Publisher: Prof. H.S. Srivastava Foundation for Science and Technology Country of Publication: India NLM ID: 101304333 Publication Model: Print-Electronic Cited Medium: Print ISSN: 0971-5894 (Print) Linking ISSN: 09740430 NLM ISO Abbreviation: Physiol Mol Biol Plants Subsets: PubMed not MEDLINE |
| Imprint Name(s): | Publication: 2008- : New Delhi : Springer India Private Ltd., : Prof. H.S. Srivastava Foundation for Science and Technology; Original Publication: Lucknow : Prof. H.S. Srivastava Foundation for Science and Technology |
| Abstract: | To establish phylogenetic relationships and estimate the intra and interspecific divergence, the amplification and the sequencing of the internal transcribed spacers of ribosomal DNA (ITS = ITS1 + 5.8S + ITS2) were analyzed in Tunisian complex Lolium-Festuca DNA. These spacer regions have evolved mainly by point mutations. Results revealed a high level of polymorphism within studied species. Significant similarities were observed between these two species and showed the existence of an important phylogenetic relationship. Besides, this molecular approach has revealed two new clusterings, with a homologous ITS gene namely: Bromus hordeaceus and Hordeum murinum subsp. This could be explained by the conservation of an ancestral ITS gene in some fescue plants. Thus, Tunisian tall fescue and perennial ryegrass may derive from Bromus hordeaceus and Hordeum murinum subsp. Considerable morphology and bioclimatic distribution similarities were discovered in ITS sequences within the same species. This study can be of great help to identify suitable accessions that could be used in local fescue and ryegrass improvement program.; (© Prof. H.S. Srivastava Foundation for Science and Society 2020.) |
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| Contributed Indexing: | Keywords: Genetic diversity; ITS; Intra and interspecific phylogeny; Ryegrass; Tall fescue; Tunisia |
| Entry Date(s): | Date Created: 20200312 Latest Revision: 20210201 |
| Update Code: | 20260130 |
| PubMed Central ID: | PMC7036405 |
| DOI: | 10.1007/s12298-019-00749-2 |
| PMID: | 32158138 |
| Database: | MEDLINE |
Journal Article