| Title: |
Rapid Purification of DNA Topoisomerase II Containing a Hexahistidine Tag by Metal Ion Affinity Chromatography. |
| Authors: |
Walker, John M.; Bjornsti, Mary-Ann1; Osheroff, Neil2; Biersack, Harald3; Jensen, Sanne4; Westergaard, Ole5 |
| Source: |
DNA Topoisomerase Protocols. 1999, p235-242. 8p. |
| Abstract: |
Since DNA topoisomerases have become a major focus for scientists, the purification of these proteins from all sources represents one of the basic hurdles on the way to investigating this important class of enzymes. A variety of methods consisting of numerous steps have been applied to obtain a reasonable amount of pure enzyme (1-4). [ABSTRACT FROM AUTHOR] |
| Database: |
Supplemental Index |