Melatonin Treatment Alters Biological and Immunomodulatory Properties of Human Dental Pulp Mesenchymal Stem Cells via Augmented Transforming Growth Factor Beta Secretion.
| Title: | Melatonin Treatment Alters Biological and Immunomodulatory Properties of Human Dental Pulp Mesenchymal Stem Cells via Augmented Transforming Growth Factor Beta Secretion. |
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| Authors: | García-Bernal D; Hematopoietic Transplant and Cellular Therapy Unit, Instituto Murciano de Investigación Biosanitaria-Arrixaca, Murcia, Spain; Internal Medicine Department, Faculty of Medicine, University of Murcia, Murcia, Spain.; López-García S; Hematopoietic Transplant and Cellular Therapy Unit, Instituto Murciano de Investigación Biosanitaria-Arrixaca, Murcia, Spain.; Sanz JL; Department of Stomatology, Faculty of Medicine and Dentistry, University of Valencia, Valencia, Spain.; Guerrero-Gironés J; School of Dentistry, Faculty of Medicine, University of Murcia, Murcia, Spain.; García-Navarro EM; Hematopoietic Transplant and Cellular Therapy Unit, Instituto Murciano de Investigación Biosanitaria-Arrixaca, Murcia, Spain.; Moraleda JM; Hematopoietic Transplant and Cellular Therapy Unit, Instituto Murciano de Investigación Biosanitaria-Arrixaca, Murcia, Spain; Internal Medicine Department, Faculty of Medicine, University of Murcia, Murcia, Spain.; Forner L; Department of Stomatology, Faculty of Medicine and Dentistry, University of Valencia, Valencia, Spain.; Rodríguez-Lozano FJ; Hematopoietic Transplant and Cellular Therapy Unit, Instituto Murciano de Investigación Biosanitaria-Arrixaca, Murcia, Spain; School of Dentistry, Faculty of Medicine, University of Murcia, Murcia, Spain. Electronic address: fcojavier@um.es. |
| Source: | Journal of endodontics [J Endod] 2021 Mar; Vol. 47 (3), pp. 424-435. Date of Electronic Publication: 2020 Dec 23. |
| Publication Type: | Journal Article |
| Language: | English |
| Journal Info: | Publisher: Elsevier Country of Publication: United States NLM ID: 7511484 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1878-3554 (Electronic) Linking ISSN: 00992399 NLM ISO Abbreviation: J Endod |
| Imprint Name(s): | Publication: New York : Elsevier; Original Publication: Chicago, American Dental Assocation, . |
| MeSH Terms: | Melatonin*/pharmacology ; Mesenchymal Stem Cells*; Cell Differentiation ; Cell Proliferation ; Cells, Cultured ; Dental Pulp ; Humans ; Osteogenesis ; Stem Cells ; Transforming Growth Factor beta |
| Abstract: | Introduction: Melatonin is an endogenous neurohormone with well-reported anti-inflammatory and antioxidant properties, but the direct biological and immunomodulatory effects of melatonin on human dental pulp stem cells (hDPSCs) has not been fully elucidated. The aim of this study was to evaluate the influence of melatonin on the cytocompatibility, proliferation, cell migration, odontogenic differentiation, mineralized nodule formation, and immunomodulatory properties of hDPSCs.; Methods: To address the melatonin biological effects on hDPSCs, the cytocompatibility, proliferation, cell migration, odontogenic differentiation, mineralized nodule formation, and immunomodulatory properties of hDPSCs after melatonin treatment were evaluated. The statistical differences were evaluated using 1-way analysis of variance with the Tukey multiple comparison test.; Results: We found that melatonin did not alter hDPSC immunophenotype or cell viability, even at the highest concentrations used. However, using intermediate melatonin concentrations (10-300 μmol/L), a significantly higher proliferation rate (P < .05 and P < .01) and migration of hDPSCs (P < .01) were observed. Importantly, melatonin treatment (100 μmol/L) significantly increased the secretion of the anti-inflammatory cytokine transforming growth factor beta (P < .05 and P < .01) and provoked a more robust antiproliferative effect on mitogen-stimulated T cells (P < .05). Finally, and unlike previous results found with mesenchymal stem cells from other sources, melatonin fails to induce or accelerate the spontaneous osteogenic differentiation of hDPSCs.; Conclusions: Together, these findings provide key data on the bioactivity of melatonin and its effects on hPDSC biological and immunomodulatory properties.; (Copyright © 2020 American Association of Endodontists. Published by Elsevier Inc. All rights reserved.) |
| Contributed Indexing: | Keywords: Dental pulp stem cells; immunomodulatory properties; melatonin; odontogenic differentiation; transforming growth factor beta |
| Substance Nomenclature: | 0 (Transforming Growth Factor beta); JL5DK93RCL (Melatonin) |
| Entry Date(s): | Date Created: 20201228 Date Completed: 20210223 Latest Revision: 20210223 |
| Update Code: | 20260130 |
| DOI: | 10.1016/j.joen.2020.12.008 |
| PMID: | 33359532 |
| Database: | MEDLINE |
Journal Article