A glucose meter interface for point-of-care gene circuit-based diagnostics.
| Title: | A glucose meter interface for point-of-care gene circuit-based diagnostics. |
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| Authors: | Amalfitano E; Leslie Dan Faculty of Pharmacy, University of Toronto, Toronto, ON, M5S 3M2, Canada.; Karlikow M; Leslie Dan Faculty of Pharmacy, University of Toronto, Toronto, ON, M5S 3M2, Canada.; Norouzi M; Leslie Dan Faculty of Pharmacy, University of Toronto, Toronto, ON, M5S 3M2, Canada.; Jaenes K; Leslie Dan Faculty of Pharmacy, University of Toronto, Toronto, ON, M5S 3M2, Canada.; Cicek S; Leslie Dan Faculty of Pharmacy, University of Toronto, Toronto, ON, M5S 3M2, Canada.; Masum F; Leslie Dan Faculty of Pharmacy, University of Toronto, Toronto, ON, M5S 3M2, Canada.; Sadat Mousavi P; Leslie Dan Faculty of Pharmacy, University of Toronto, Toronto, ON, M5S 3M2, Canada.; Guo Y; Leslie Dan Faculty of Pharmacy, University of Toronto, Toronto, ON, M5S 3M2, Canada.; Tang L; Leslie Dan Faculty of Pharmacy, University of Toronto, Toronto, ON, M5S 3M2, Canada.; Sydor A; Program in Cell Biology, Hospital for Sick Children, Peter Gilgan Center for Research and Learning, Toronto, ON, M5G 0A4, Canada.; Ma D; Biodesign Center for Molecular Design and Biomimetics, The Biodesign Institute and the School of Molecular Sciences, Arizona State University, AZ, 85287, USA.; Pearson JD; Lunenfeld Tanenbaum Research Institute, Mt Sinai Hospital, Sinai Health System, Toronto, M5G 1X5, ON, Canada.; Department of Ophthalmology and Vision Science, University of Toronto, Toronto, M5T 3A9, ON, Canada.; Department of Laboratory Medicine and Pathobiology, University of Toronto, Toronto, M5S 1A8, ON, Canada.; Trcka D; Lunenfeld Tanenbaum Research Institute, Mt Sinai Hospital, Sinai Health System, Toronto, M5G 1X5, ON, Canada.; Pinette M; Canadian Food Inspection Agency, National Centre for Foreign Animal Disease, Winnipeg, R3E 3M4, MB, Canada.; Ambagala A; Canadian Food Inspection Agency, National Centre for Foreign Animal Disease, Winnipeg, R3E 3M4, MB, Canada.; Babiuk S; Canadian Food Inspection Agency, National Centre for Foreign Animal Disease, Winnipeg, R3E 3M4, MB, Canada.; Pickering B; Canadian Food Inspection Agency, National Centre for Foreign Animal Disease, Winnipeg, R3E 3M4, MB, Canada.; Department of Medical Microbiology and Infectious Diseases, Faculty of Medicine, University of Manitoba, Winnipeg, R3E 0J9, MB, Canada.; Iowa State University, College of Veterinary Medicine, Department of Veterinary Microbiology and Preventive Medicine, Ames, IA, 50011, USA.; Wrana J; Lunenfeld Tanenbaum Research Institute, Mt Sinai Hospital, Sinai Health System, Toronto, M5G 1X5, ON, Canada.; Department of Molecular Genetics, University of Toronto, Toronto, M5S 1A8, ON, Canada.; Bremner R; Lunenfeld Tanenbaum Research Institute, Mt Sinai Hospital, Sinai Health System, Toronto, M5G 1X5, ON, Canada.; Department of Ophthalmology and Vision Science, University of Toronto, Toronto, M5T 3A9, ON, Canada.; Department of Laboratory Medicine and Pathobiology, University of Toronto, Toronto, M5S 1A8, ON, Canada.; Mazzulli T; Department of Laboratory Medicine and Pathobiology, University of Toronto, Toronto, M5S 1A8, ON, Canada.; Department of Microbiology, Sinai Health System/University Health Network, Toronto, M5G 1X5, ON, Canada.; Sinton D; Department of Mechanical and Industrial Engineering, University of Toronto, Toronto, M5S 3G8, ON, Canada.; Brumell JH; Program in Cell Biology, Hospital for Sick Children, Peter Gilgan Center for Research and Learning, Toronto, ON, M5G 0A4, Canada.; Department of Molecular Genetics, University of Toronto, Toronto, M5S 1A8, ON, Canada.; Institute of Medical Science, University of Toronto, Toronto, M5S 1A8, ON, Canada.; SickKids IBD Centre, Hospital for Sick Children, Toronto, M5G 1X8, ON, Canada.; Green AA; Biodesign Center for Molecular Design and Biomimetics, The Biodesign Institute and the School of Molecular Sciences, Arizona State University, AZ, 85287, USA.; Department of Biomedical Engineering, Boston University, Boston, MA, 02215, USA.; Pardee K; Leslie Dan Faculty of Pharmacy, University of Toronto, Toronto, ON, M5S 3M2, Canada. keith.pardee@utoronto.ca.; Department of Mechanical and Industrial Engineering, University of Toronto, Toronto, M5S 3G8, ON, Canada. keith.pardee@utoronto.ca. |
| Source: | Nature communications [Nat Commun] 2021 Feb 01; Vol. 12 (1), pp. 724. Date of Electronic Publication: 2021 Feb 01. |
| Publication Type: | Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S. |
| Language: | English |
| Journal Info: | Publisher: Nature Pub. Group Country of Publication: England NLM ID: 101528555 Publication Model: Electronic Cited Medium: Internet ISSN: 2041-1723 (Electronic) Linking ISSN: 20411723 NLM ISO Abbreviation: Nat Commun Subsets: MEDLINE |
| Imprint Name(s): | Original Publication: [London] : Nature Pub. Group |
| MeSH Terms: | Point-of-Care Systems* ; Point-of-Care Testing*; Biosensing Techniques/*methods ; Gene Regulatory Networks/*genetics ; Glucose/*analysis ; Nucleic Acids/*analysis; Biosensing Techniques/instrumentation ; COVID-19/diagnosis ; COVID-19/epidemiology ; COVID-19/virology ; Glucose/metabolism ; Nucleic Acids/genetics ; SARS-CoV-2/isolation & purification ; SARS-CoV-2/physiology ; Typhoid Fever/blood ; Typhoid Fever/diagnosis ; Typhoid Fever/microbiology ; Humans ; Pandemics |
| Abstract: | Recent advances in cell-free synthetic biology have given rise to gene circuit-based sensors with the potential to provide decentralized and low-cost molecular diagnostics. However, it remains a challenge to deliver this sensing capacity into the hands of users in a practical manner. Here, we leverage the glucose meter, one of the most widely available point-of-care sensing devices, to serve as a universal reader for these decentralized diagnostics. We describe a molecular translator that can convert the activation of conventional gene circuit-based sensors into a glucose output that can be read by off-the-shelf glucose meters. We show the development of new glucogenic reporter systems, multiplexed reporter outputs and detection of nucleic acid targets down to the low attomolar range. Using this glucose-meter interface, we demonstrate the detection of a small-molecule analyte; sample-to-result diagnostics for typhoid, paratyphoid A/B; and show the potential for pandemic response with nucleic acid sensors for SARS-CoV-2. |
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| Grant Information: | 201610FDN-375469 Canada CIHR; 950-231075 Canada CIHR; P41 RR005351 United States RR NCRR NIH HHS; R21 AI136571 United States AI NIAID NIH HHS; U01 AI148319 United States AI NIAID NIH HHS; DP2 GM126892 United States GM NIGMS NIH HHS |
| Substance Nomenclature: | 0 (Nucleic Acids); IY9XDZ35W2 (Glucose) |
| Entry Date(s): | Date Created: 20210202 Date Completed: 20210219 Latest Revision: 20210219 |
| Update Code: | 20260130 |
| PubMed Central ID: | PMC7851131 |
| DOI: | 10.1038/s41467-020-20639-6 |
| PMID: | 33526784 |
| Database: | MEDLINE |
Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.