Hypothermic oxygenated machine perfusion of the human pancreas for clinical islet isolation: a prospective feasibility study.
| Title: | Hypothermic oxygenated machine perfusion of the human pancreas for clinical islet isolation: a prospective feasibility study. |
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| Authors: | Doppenberg JB; Transplantation Center, Leiden University Medical Center, Leiden, the Netherlands.; Leemkuil M; Department of Surgery, University Medical Center Groningen, Groningen, the Netherlands.; Engelse MA; Transplantation Center, Leiden University Medical Center, Leiden, the Netherlands.; Krikke C; Department of Surgery, University Medical Center Groningen, Groningen, the Netherlands.; de Koning EJP; Transplantation Center, Leiden University Medical Center, Leiden, the Netherlands.; Leuvenink HGD; Department of Surgery, University Medical Center Groningen, Groningen, the Netherlands. |
| Source: | Transplant international : official journal of the European Society for Organ Transplantation [Transpl Int] 2021 Aug; Vol. 34 (8), pp. 1397-1407. Date of Electronic Publication: 2021 Jul 04. |
| Publication Type: | Journal Article |
| Language: | English |
| Journal Info: | Publisher: Frontiers Media S. A Country of Publication: Switzerland NLM ID: 8908516 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1432-2277 (Electronic) Linking ISSN: 09340874 NLM ISO Abbreviation: Transpl Int Subsets: MEDLINE |
| Imprint Name(s): | Publication: 2022- : Lausanne, Switzerland : Frontiers Media S. A.; Original Publication: [Heidelberg, FRG] : Springer International, 1988- |
| MeSH Terms: | Diabetes Mellitus, Experimental* ; Organ Preservation*; Animals ; Feasibility Studies ; Humans ; Mice ; Pancreas ; Perfusion ; Prospective Studies |
| Abstract: | Due to an increasing scarcity of pancreases with optimal donor characteristics, islet isolation centers utilize pancreases from extended criteria donors, such as from donation after circulatory death (DCD) donors, which are particularly susceptible to prolonged cold ischemia time (CIT). We hypothesized that hypothermic machine perfusion (HMP) can safely increase CIT. Five human DCD pancreases were subjected to 6 h of oxygenated HMP. Perfusion parameters, apoptosis, and edema were measured prior to islet isolation. Five human DBD pancreases were evaluated after static cold storage (SCS). Islet viability, and in vitro and in vivo functionality in diabetic mice were analyzed. Islets were isolated from HMP pancreases after 13.4 h [12.9-14.5] CIT and after 9.2 h [6.5-12.5] CIT from SCS pancreases. Histological analysis of the pancreatic tissue showed that HMP did not induce edema nor apoptosis. Islets maintained >90% viable during culture, and an appropriate in vitro and in vivo function in mice was demonstrated after HMP. The current study design does not permit to demonstrate that oxygenated HMP allows for cold ischemia extension; however, the successful isolation of functional islets from discarded human DCD pancreases after performing 6 h of oxygenated HMP indicates that oxygenated HMP may be a useful technology for better preservation of pancreases.; (© 2021 The Authors. Transplant International published by John Wiley & Sons Ltd on behalf of Steunstichting ESOT.) |
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| Grant Information: | This project was financially supported by the Diabetes Cell Therapy Initiative (DCTI) FES 2009 program LSH-DCTI including the Dutch Diabetes Research Foundation. |
| Contributed Indexing: | Keywords: donation after circulatory death; hypothermic machine perfusion; islet isolation |
| Entry Date(s): | Date Created: 20210526 Date Completed: 20211018 Latest Revision: 20260617 |
| Update Code: | 20260618 |
| PubMed Central ID: | PMC8456912 |
| DOI: | 10.1111/tri.13927 |
| PMID: | 34036616 |
| Database: | MEDLINE |
Journal Article