Rescue of streptomycin activity by piperine in Mycobacterium tuberculosis.
| Title: | Rescue of streptomycin activity by piperine in Mycobacterium tuberculosis. |
|---|---|
| Authors: | Calsavara LL; Postgraduate Program in Bioscience & Physiopathology, State University of Maringá (UEM), Maringá, Paraná, Brazil.; Department of Clinical Analysis & Biomedicine, Laboratory of Medical Bacteriology, State University of Maringa, Parana, Brazil.; Hegeto LA; Postgraduate Program in Health Sciences, State University of Maringá (UEM), Maringá, Paraná, Brazil.; Department of Clinical Analysis & Biomedicine, Laboratory of Medical Bacteriology, State University of Maringa, Parana, Brazil.; Sampiron EG; Postgraduate Program in Health Sciences, State University of Maringá (UEM), Maringá, Paraná, Brazil.; Department of Clinical Analysis & Biomedicine, Laboratory of Medical Bacteriology, State University of Maringa, Parana, Brazil.; Costacurta GF; Postgraduate Program in Health Sciences, State University of Maringá (UEM), Maringá, Paraná, Brazil.; Department of Clinical Analysis & Biomedicine, Laboratory of Medical Bacteriology, State University of Maringa, Parana, Brazil.; Murase LS; Postgraduate Program in Health Sciences, State University of Maringá (UEM), Maringá, Paraná, Brazil.; Department of Clinical Analysis & Biomedicine, Laboratory of Medical Bacteriology, State University of Maringa, Parana, Brazil.; Souza JV; Postgraduate Program in Bioscience & Physiopathology, State University of Maringá (UEM), Maringá, Paraná, Brazil.; Department of Clinical Analysis & Biomedicine, Laboratory of Medical Bacteriology, State University of Maringa, Parana, Brazil.; de Almeida AL; Postgraduate Program in Bioscience & Physiopathology, State University of Maringá (UEM), Maringá, Paraná, Brazil.; Department of Clinical Analysis & Biomedicine, Laboratory of Medical Bacteriology, State University of Maringa, Parana, Brazil.; de S Santos NC; Postgraduate Program in Bioscience & Physiopathology, State University of Maringá (UEM), Maringá, Paraná, Brazil.; Department of Clinical Analysis & Biomedicine, Laboratory of Medical Bacteriology, State University of Maringa, Parana, Brazil.; Siqueira VL; Postgraduate Program in Bioscience & Physiopathology, State University of Maringá (UEM), Maringá, Paraná, Brazil.; Department of Clinical Analysis & Biomedicine, Laboratory of Medical Bacteriology, State University of Maringa, Parana, Brazil.; de L Scodro RB; Postgraduate Program in Health Sciences, State University of Maringá (UEM), Maringá, Paraná, Brazil.; Department of Clinical Analysis & Biomedicine, Laboratory of Medical Bacteriology, State University of Maringa, Parana, Brazil.; Cardoso RF; Postgraduate Program in Bioscience & Physiopathology, State University of Maringá (UEM), Maringá, Paraná, Brazil.; Postgraduate Program in Health Sciences, State University of Maringá (UEM), Maringá, Paraná, Brazil.; Department of Clinical Analysis & Biomedicine, Laboratory of Medical Bacteriology, State University of Maringa, Parana, Brazil.; Caleffi-Ferracioli KR; Postgraduate Program in Bioscience & Physiopathology, State University of Maringá (UEM), Maringá, Paraná, Brazil.; Department of Clinical Analysis & Biomedicine, Laboratory of Medical Bacteriology, State University of Maringa, Parana, Brazil. |
| Source: | Future microbiology [Future Microbiol] 2021 Jun; Vol. 16, pp. 623-633. Date of Electronic Publication: 2021 Jun 08. |
| Publication Type: | Journal Article; Research Support, Non-U.S. Gov't |
| Language: | English |
| Journal Info: | Publisher: Taylor & Francis Country of Publication: England NLM ID: 101278120 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1746-0921 (Electronic) Linking ISSN: 17460913 NLM ISO Abbreviation: Future Microbiol Subsets: MEDLINE |
| Imprint Name(s): | Publication: 2024- : [Milton Park, Oxfordshire] : Taylor & Francis; Original Publication: London : Future Medicine |
| MeSH Terms: | Alkaloids/*pharmacology ; Antitubercular Agents/*pharmacology ; Benzodioxoles/*pharmacology ; Mycobacterium tuberculosis/*drug effects ; Piperidines/*pharmacology ; Polyunsaturated Alkamides/*pharmacology ; Streptomycin/*pharmacology; Bacterial Proteins/genetics ; Bacterial Proteins/metabolism ; Drug Resistance, Bacterial/drug effects ; Membrane Transport Proteins/genetics ; Membrane Transport Proteins/metabolism ; Drug Synergism ; Humans ; Microbial Sensitivity Tests |
| Abstract: | Aim: To evaluate the modulatory effect of piperine (PIP) on streptomycin (SM) activity in Mycobacterium tuberculosis (Mtb). Materials & methods: SM and PIP minimum inhibitory concentration (MIC) and combinatory activity were determined in Mtb H37Rv and in susceptible and resistant clinical isolates. Ethidium bromide accumulation assay and relative quantification of efflux pumps genes (rv1258c, rv1218c and rv2942), after SM and SM+PIP combination exposure, were also performed. Results: PIP concentration of 25 μg/ml (1/4× MIC) was able to inhibit efflux pumps activity, to modulate SM activity in Mtb, and conducted changes in the relative quantification of efflux pumps genes. Conclusion: SM+PIP combination was able to rescue the SM-susceptible MIC values in SM-resistant Mtb. |
| Contributed Indexing: | Keywords: Mycobacterium tuberculosis; efflux pump; modulatory effect; piperine; streptomycin |
| Substance Nomenclature: | 0 (Alkaloids); 0 (Antitubercular Agents); 0 (Bacterial Proteins); 0 (Benzodioxoles); 0 (Membrane Transport Proteins); 0 (Piperidines); 0 (Polyunsaturated Alkamides); U71XL721QK (piperine); Y45QSO73OB (Streptomycin) |
| Entry Date(s): | Date Created: 20210608 Date Completed: 20211209 Latest Revision: 20211214 |
| Update Code: | 20260130 |
| DOI: | 10.2217/fmb-2020-0124 |
| PMID: | 34098743 |
| Database: | MEDLINE |
Journal Article; Research Support, Non-U.S. Gov't