Alterations in endogenous hormone levels and energy metabolism promoted the induction, differentiation and maturation of Begonia somatic embryos under clinorotation.
| Title: | Alterations in endogenous hormone levels and energy metabolism promoted the induction, differentiation and maturation of Begonia somatic embryos under clinorotation. |
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| Authors: | Khai HD; Tay Nguyen Institute for Scientific Research, Vietnam Academy of Science and Technology, Viet Nam.; Bien LT; Tay Nguyen Institute for Scientific Research, Vietnam Academy of Science and Technology, Viet Nam; Graduate University of Science and Technology, Vietnam Academy of Science and Technology, Viet Nam.; Vinh NQ; Tay Nguyen University, Viet Nam.; Dung DM; Tay Nguyen University, Viet Nam.; Nghiep ND; University of Science, Vietnam National University, Ho Chi Minh City, Viet Nam.; Mai NTN; Tay Nguyen Institute for Scientific Research, Vietnam Academy of Science and Technology, Viet Nam.; Tung HT; Tay Nguyen Institute for Scientific Research, Vietnam Academy of Science and Technology, Viet Nam.; Luan VQ; Tay Nguyen Institute for Scientific Research, Vietnam Academy of Science and Technology, Viet Nam.; Cuong DM; Tay Nguyen Institute for Scientific Research, Vietnam Academy of Science and Technology, Viet Nam.; Nhut DT; Tay Nguyen Institute for Scientific Research, Vietnam Academy of Science and Technology, Viet Nam. Electronic address: duongtannhut@gmail.com. |
| Source: | Plant science : an international journal of experimental plant biology [Plant Sci] 2021 Nov; Vol. 312, pp. 111045. Date of Electronic Publication: 2021 Aug 28. |
| Publication Type: | Journal Article |
| Language: | English |
| Journal Info: | Publisher: Elsevier Ireland Country of Publication: Ireland NLM ID: 9882015 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1873-2259 (Electronic) Linking ISSN: 01689452 NLM ISO Abbreviation: Plant Sci Subsets: MEDLINE |
| Imprint Name(s): | Publication: - : Shannon, Co. Clare : Elsevier Ireland; Original Publication: Shannon [Clare] : Elsevier Scientific Publishers Ireland Ltd., c1985- |
| MeSH Terms: | Energy Metabolism*; Abscisic Acid/*metabolism ; Begoniaceae/*growth & development ; Begoniaceae/*metabolism ; Cell Differentiation/*drug effects ; Plant Growth Regulators/*metabolism ; Seeds/*growth & development ; Seeds/*metabolism; Cell Culture Techniques ; Plant Somatic Embryogenesis Techniques |
| Abstract: | The present study provides a visual insight into the effects of simulated microgravity (MG) on somatic embryogenesis (SE) in Begonia through the analysis of phytohormone fluctuations and energy metabolism. To investigate this relationship, thin cell layer culture model was first used. The results showed that MG changed the phytohormone content and stimulated starch biosynthesis to convert into sugar to release energy needed for regeneration and proliferation. Moreover, from the results it is likely that MG accelerated the initiation and subsequently maturation and aging of SE via decrease of AUX and increase of ABA. High content of GA, CKs, starch, sugar and low ABA as well as high CKs/ABA ratio were responsible for the increase in the number of embryos under clinorotation which was 1.57-fold higher than control after 90 days. The increase in fresh and dry weight of somatic embryos and chlorophyll content under MG were confirmed as their adaptive responses to gravitational stress. However, long-term exposure to MG (120 days) stimulated biosynthesis of ABA levels 1.85-fold higher than controls, which resulted in a decrease in chlorophyll content, increase in number of mature embryos and stomata length. These results revealed that MG regulated the induction, differentiation and senescence of somatic embryos via a biochemical interaction pathway.; (Copyright © 2021 Elsevier B.V. All rights reserved.) |
| Contributed Indexing: | Keywords: Aging; Cacbohydrate; Clinorotation; Endogenous hormone; Somatic embryo; Thin cell layer technique |
| Substance Nomenclature: | 0 (Plant Growth Regulators); 72S9A8J5GW (Abscisic Acid) |
| Entry Date(s): | Date Created: 20211008 Date Completed: 20211109 Latest Revision: 20211109 |
| Update Code: | 20260130 |
| DOI: | 10.1016/j.plantsci.2021.111045 |
| PMID: | 34620443 |
| Database: | MEDLINE |
Journal Article