Vitamin A deficiency affects gene expression in the Drosophila melanogaster head.
| Title: | Vitamin A deficiency affects gene expression in the Drosophila melanogaster head. |
|---|---|
| Authors: | Dewett D; Department of Biology, University of Massachusetts Boston, Boston, MA 02125, USA.; Labaf M; Department of Mathematics, University of Massachusetts Boston, Boston, MA 02125, USA.; Lam-Kamath K; Department of Biology, University of Massachusetts Boston, Boston, MA 02125, USA.; Zarringhalam K; Department of Mathematics, University of Massachusetts Boston, Boston, MA 02125, USA.; Rister J; Department of Biology, University of Massachusetts Boston, Boston, MA 02125, USA. |
| Source: | G3 (Bethesda, Md.) [G3 (Bethesda)] 2021 Oct 19; Vol. 11 (11). |
| Publication Type: | Journal Article; Research Support, N.I.H., Extramural |
| Language: | English |
| Journal Info: | Publisher: Oxford University Press Country of Publication: England NLM ID: 101566598 Publication Model: Print Cited Medium: Internet ISSN: 2160-1836 (Electronic) Linking ISSN: 21601836 NLM ISO Abbreviation: G3 (Bethesda) Subsets: MEDLINE |
| Imprint Name(s): | Publication: 2021- : [Oxford] : Oxford University Press; Original Publication: Bethesda, MD : Genetics Society of America, 2011- |
| MeSH Terms: | Drosophila Proteins* ; Vitamin A Deficiency*; Drosophila melanogaster/genetics ; Rhodopsin/genetics ; Animals ; Gene Expression ; Vitamin A |
| Abstract: | Insufficient dietary intake of vitamin A causes various human diseases. For instance, chronic vitamin A deprivation causes blindness, slow growth, impaired immunity, and an increased risk of mortality in children. In contrast to these diverse effects of vitamin A deficiency (VAD) in mammals, chronic VAD in flies neither causes obvious developmental defects nor lethality. As in mammals, VAD in flies severely affects the visual system: it impairs the synthesis of the retinal chromophore, disrupts the formation of the visual pigments (Rhodopsins), and damages the photoreceptors. However, the molecular mechanisms that respond to VAD remain poorly understood. To identify genes and signaling pathways that are affected by VAD, we performed RNA-sequencing and differential gene expression analysis in Drosophila melanogaster. We found an upregulation of genes that are essential for the synthesis of the retinal chromophore, specific aminoacyl-tRNA synthetases, and major nutrient reservoir proteins. We also discovered that VAD affects several genes that are required for the termination of the light response: for instance, we found a downregulation of both arrestin genes that are essential for the inactivation of Rhodopsin. A comparison of the VAD-responsive genes with previously identified blue light stress-responsive genes revealed that the two types of environmental stress trigger largely nonoverlapping transcriptome responses. Yet, both stresses increase the expression of seven genes with poorly understood functions. Taken together, our transcriptome analysis offers insights into the molecular mechanisms that respond to environmental stresses.; (© The Author(s) 2021. Published by Oxford University Press on behalf of Genetics Society of America.) |
| References: | Bioinformatics. 2014 Apr 1;30(7):923-30. (PMID: 24227677); Nucleic Acids Res. 2012 May;40(10):4288-97. (PMID: 22287627); J Biol Chem. 2000 Apr 21;275(16):11915-20. (PMID: 10766819); Neuron. 2006 Jan 19;49(2):229-41. (PMID: 16423697); Proc Natl Acad Sci U S A. 1958 Jul 15;44(7):648-61. (PMID: 16590255); J Vis Exp. 2012 Nov 14;(69):4347. (PMID: 23183823); Biochem Biophys Res Commun. 2008 Sep 19;374(2):299-303. (PMID: 18627767); Genetics. 1994 Oct;138(2):413-21. (PMID: 7828824); Vis Neurosci. 1992 Nov;9(5):461-9. (PMID: 1450100); Eur J Cell Biol. 1994 Apr;63(2):219-29. (PMID: 8082646); Sci Rep. 2016 Jan 25;6:19692. (PMID: 26805723); J Biol Chem. 2006 Apr 7;281(14):9205-9. (PMID: 16464863); Neuron. 2002 Mar 28;34(1):83-93. (PMID: 11931743); Dev Biol. 2021 Aug;476:68-78. (PMID: 33774009); Curr Opin Neurobiol. 2015 Oct;34:37-45. (PMID: 25638280); Microsc Res Tech. 1996 Dec 15;35(6):418-30. (PMID: 9016446); Proc Natl Acad Sci U S A. 2013 May 7;110(19):E1752-60. (PMID: 23613578); BMB Rep. 2015 Aug;48(8):445-53. (PMID: 25999177); Subcell Biochem. 2016;81:231-259. (PMID: 27830507); Biochim Biophys Acta. 2005 May 30;1740(2):108-15. (PMID: 15949676); Sci Rep. 2019 Sep 3;9(1):12714. (PMID: 31481676); Proc Natl Acad Sci U S A. 2001 Jan 30;98(3):1130-5. (PMID: 11158606); Genome Biol. 2014;15(12):550. (PMID: 25516281); J Neurosci. 2015 Feb 11;35(6):2731-46. (PMID: 25673862); Proc Natl Acad Sci U S A. 1960 May;46(5):587-608. (PMID: 16590647); Antioxidants (Basel). 2018 Jan 01;7(1):. (PMID: 29301252); Nature. 1990 Sep 20;347(6290):298-301. (PMID: 2169594); Sci Rep. 2018 Jan 30;8(1):1908. (PMID: 29382949); Nucleic Acids Res. 2019 Jul 2;47(W1):W191-W198. (PMID: 31066453); Front Mol Neurosci. 2017 Mar 07;10:57. (PMID: 28326013); J Neurosci. 2006 Aug 16;26(33):8570-7. (PMID: 16914683); Neuron. 2001 Nov 20;32(4):657-71. (PMID: 11719206); J Nutr. 2008 Oct;138(10):1835-9. (PMID: 18806089); PeerJ. 2016 Sep 06;4:e2427. (PMID: 27651995); J Physiol. 1994 Oct 15;480 ( Pt 2):261-79. (PMID: 7532713); J Biol Chem. 2001 Apr 27;276(17):14110-6. (PMID: 11278918); Curr Biol. 2010 Jan 26;20(2):93-102. (PMID: 20045325); Nat Methods. 2012 Jun 28;9(7):676-82. (PMID: 22743772); Arch Biochem Biophys. 2001 Jan 1;385(1):47-52. (PMID: 11361025); Proc Natl Acad Sci U S A. 2008 Dec 2;105(48):19000-5. (PMID: 19020100); Science. 1991 Dec 6;254(5037):1478-84. (PMID: 1962207); Dev Biol. 1990 Nov;142(1):138-46. (PMID: 2227091); Cell Biosci. 2012 Mar 22;2(1):11. (PMID: 22439772); J Biol Chem. 2013 Mar 29;288(13):9017-27. (PMID: 23393141); FASEB J. 1995 Dec;9(15):1551-8. (PMID: 8529834); EMBO J. 1994 Oct 17;13(20):4886-95. (PMID: 7957056); J Cell Sci. 2016 Jan 15;129(2):394-405. (PMID: 26598556); Proc Natl Acad Sci U S A. 1993 Feb 15;90(4):1536-40. (PMID: 8434015); Development. 2013 Feb 1;140(3):493-503. (PMID: 23293281); Invest Ophthalmol Vis Sci. 2001 Feb;42(2):497-505. (PMID: 11157889); J Gen Physiol. 1988 Nov;92(5):587-600. (PMID: 3148683); J Insect Physiol. 1977;23(7):871-8. (PMID: 415089); Proc Natl Acad Sci U S A. 1993 Feb 15;90(4):1531-5. (PMID: 8094559); Eur J Biochem. 1997 Apr 1;245(1):191-8. (PMID: 9128741); Bioinformatics. 2010 Jan 1;26(1):139-40. (PMID: 19910308); J Biol Chem. 1985 Oct 15;260(23):12670-4. (PMID: 3930500); Biochim Biophys Acta Mol Cell Biol Lipids. 2020 Jul;1865(7):158683. (PMID: 32169653); Neuron. 1993 Jun;10(6):1113-9. (PMID: 8318232); J Biol Chem. 2007 Nov 16;282(46):33553-33561. (PMID: 17855355); Bioinformatics. 2013 Jan 1;29(1):15-21. (PMID: 23104886); Nucleic Acids Res. 2018 Jan 4;46(D1):D809-D815. (PMID: 29069479); Biophys J. 1997 Dec;73(6):3182-91. (PMID: 9414230); Environ Health Perspect. 2005 Dec;113(12):A822-7. (PMID: 16330339); Mol Aspects Med. 2005 Dec;26(6):459-516. (PMID: 16309738); Neuron. 2006 Feb 16;49(4):533-46. (PMID: 16476663); Curr Eye Res. 1984 Jan;3(1):165-74. (PMID: 6690219); Sci Signal. 2018 Sep 04;11(546):. (PMID: 30181241); Cell. 1995 Jun 16;81(6):841-8. (PMID: 7781061); Bioessays. 2006 Apr;28(4):344-54. (PMID: 16547945); BMC Neurosci. 2018 Jul 20;19(1):43. (PMID: 30029619); Curr Biol. 2005 Jul 12;15(13):1228-34. (PMID: 16005297); Nutr Res. 2014 Nov;34(11):907-29. (PMID: 25134454); Mol Biol Evol. 2003 Apr;20(4):541-53. (PMID: 12654933); J Neurobiol. 1992 Aug;23(6):605-26. (PMID: 1431838); Eur J Biochem. 1991 Jan 1;195(1):195-201. (PMID: 1703957); Neuron. 1992 Apr;8(4):631-42. (PMID: 1314616); J Biol Chem. 2002 Jun 28;277(26):23942-8. (PMID: 11960992); Science. 1993 Jun 25;260(5116):1910-6. (PMID: 8316831); J Biol Chem. 2012 Jan 13;287(3):1627-34. (PMID: 22074927); Neuron. 2008 Jan 10;57(1):56-68. (PMID: 18184564); J Biol Chem. 2010 Jan 15;285(3):2130-9. (PMID: 19889630); Nature. 1977 Apr 14;266(5603):648-50. (PMID: 404571); Neuron. 2010 Sep 23;67(6):997-1008. (PMID: 20869596); Neuron. 1989 Apr;2(4):1313-23. (PMID: 2516726) |
| Grant Information: | R01 EY029659 United States EY NEI NIH HHS; U54 CA156734 United States CA NCI NIH HHS |
| Contributed Indexing: | Keywords: Drosophila; carotene; chromophore; photoreceptor; phototransduction; retinoic acid; rhabdomere; rhodopsin; transcriptome; vision; visual pigment; vitamin A |
| Substance Nomenclature: | 0 (Drosophila Proteins); 11103-57-4 (Vitamin A); 9009-81-8 (Rhodopsin) |
| Entry Date(s): | Date Created: 20211201 Date Completed: 20220106 Latest Revision: 20240508 |
| Update Code: | 20260130 |
| PubMed Central ID: | PMC8527478 |
| DOI: | 10.1093/g3journal/jkab297 |
| PMID: | 34849795 |
| Database: | MEDLINE |
Journal Article; Research Support, N.I.H., Extramural