Evaluation of the interaction between anti-apoptotic Bcl-2 protein family mRNA expression and autophagy gene Bcl-1 expression in Egyptian SLE patients.
| Title: | Evaluation of the interaction between anti-apoptotic Bcl-2 protein family mRNA expression and autophagy gene Bcl-1 expression in Egyptian SLE patients. |
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| Authors: | Khalil NM; Internal Medicine Department, Rheumatology and Clinical Immunology Unit, KasrAlainy School of Medicine, 63527Cairo University, Egypt.; Yousef RN; Department of Clinical and Chemical Pathology, 68787National Research Centre, Giza, Egypt.; AlDeen HG; Department of Clinical and Chemical Pathology, 68787National Research Centre, Giza, Egypt.; Behiry ME; Internal Medicine Department, Rheumatology and Clinical Immunology Unit, KasrAlainy School of Medicine, 63527Cairo University, Egypt.; Ashmawy I; Department of Clinical and Chemical Pathology, 68787National Research Centre, Giza, Egypt.; Ramadan A; Department of Molecular Genetics and Enzymology, National Research Centre, Giza, Egypt.; Awadallah E; Department of Clinical and Chemical Pathology, 68787National Research Centre, Giza, Egypt.; Ali A; Chest Department, 68103Abbassia Chest Hospital, Ministry of Health and Population, Cairo, Egypt.; Alnaggar AR; Internal Medicine Department, Rheumatology and Clinical Immunology Unit, KasrAlainy School of Medicine, 63527Cairo University, Egypt. |
| Source: | Lupus [Lupus] 2022 Sep; Vol. 31 (10), pp. 1186-1190. Date of Electronic Publication: 2022 Jun 03. |
| Publication Type: | Journal Article |
| Language: | English |
| Journal Info: | Publisher: SAGE Publications Country of Publication: England NLM ID: 9204265 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1477-0962 (Electronic) Linking ISSN: 09612033 NLM ISO Abbreviation: Lupus Subsets: MEDLINE |
| Imprint Name(s): | Publication: London : SAGE Publications; Original Publication: Houndmills, Basingstoke, Hampshire, UK : Scientific & Medical Division, Macmillan Press Ltd., c1991- |
| MeSH Terms: | Lupus Erythematosus, Systemic*/diagnosis; Apoptosis Regulatory Proteins/genetics ; Autophagy/genetics ; Beclin-1/genetics ; RNA, Messenger/genetics ; Egypt ; Humans |
| Abstract: | Objectives: Autophagy is a complex cellular process that maintains homeostasis in systemic lupus erythematosus. Abnormally high expression of Bcl-2 was observed in B and T lymphocytes in the peripheral blood in SLE patients. These may be responsible for the survival of self-reactive lymphocytes and the development of lupus, and the study aims at evaluating interaction between apoptosis and autophagy in Egyptian lupus patients.; Methods: Sixty patients with SLE were diagnosed by fulfilling the Systemic Lupus International Collaborating Clinics (SLICC) classification criteria for SLE and sixty healthy age and sex matched control. All patients were subjected to full medical history and clinical examination. Activity was assessed using SLEDAI-2K score. Gene expression of Beclin-1, Bcl-2-L2, and Bcl-2 was measured.; Results: The study revealed that the expression of anti-apoptotic Bcl-2 and Bcl-2-L2 was significantly higher in SLE patients than control subjects, as well as the major apoptotic agent (Beclin-1) mRNA, p = 0.03, < 0.001 and 0.02, respectively. The apoptotic Beclin-1 mRNA was positively correlated with SLE disease severity index, r = 0.25; p = 0.0.4; therefore, our results showed that expression of the Beclin-1 was significantly higher in SLE patients than control (p < 0.02).; Conclusion: Our results showed that expression of the Beclin 1 were significantly higher in SLE patients than control (p < 0.02). |
| Contributed Indexing: | Keywords: anti-apoptotic Bcl-2; autophagy Beclin-1; systemic lupus erythematosus |
| Substance Nomenclature: | 0 (Apoptosis Regulatory Proteins); 0 (Beclin-1); 0 (RNA, Messenger) |
| Entry Date(s): | Date Created: 20220603 Date Completed: 20220907 Latest Revision: 20250728 |
| Update Code: | 20260130 |
| DOI: | 10.1177/09612033221106302 |
| PMID: | 35657769 |
| Database: | MEDLINE |
Journal Article