Comparative evaluation of antimicrobial efficacy of calcium hydroxide, triple antibiotic paste and bromelain against Enterococcus faecalis: An In Vitro study.
| Title: | Comparative evaluation of antimicrobial efficacy of calcium hydroxide, triple antibiotic paste and bromelain against Enterococcus faecalis: An In Vitro study. |
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| Authors: | Chandwani ND; Department of Dentistry, All India Institute of Medical Sciences. Nagpur, Maharashtra, India.; Maurya N; Department of Conservative Dentistry and Endodontics, Chhattisgarh Dental College and Research Institute, Chhattisgarh, India.; Nikhade P; Department of Conservative Dentistry and Endodontics, Sharad Pawar Dental College, Datta Meghe Institute of Medical Sciences, Deemed University, Wardha, Maharashtra, India.; Chandwani J; Department of Computer Sciences and Engineering, Ramdeobaba College of Engineering and Management, Nagpur, Maharashtra, India. |
| Source: | Journal of conservative dentistry : JCD [J Conserv Dent] 2022 Jan-Feb; Vol. 25 (1), pp. 63-67. Date of Electronic Publication: 2022 May 02. |
| Publication Type: | Journal Article |
| Language: | English |
| Journal Info: | Publisher: Medknow Country of Publication: India NLM ID: 101147009 Publication Model: Print-Electronic Cited Medium: Print ISSN: 0972-0707 (Print) Linking ISSN: 09720707 NLM ISO Abbreviation: J Conserv Dent Subsets: PubMed not MEDLINE |
| Imprint Name(s): | Publication: 2008- : Mumbai Medknow; Original Publication: [Amritsar] : Federation of Operative Dentistry (India) |
| Abstract: | Context: Considering the effectiveness and safety concerns of synthetic intracanal medicaments (ICM), alternative usage of herbal ingredients in the root canal treatment may prove to be beneficial. Hence, the naturally available potent proteolytic, anti-inflammatory, and antimicrobial pineapple extract Bromelain as an ICMs needs to be studied.; Aim: The aim of this study is to evaluate and compare the antimicrobial efficacy of calcium hydroxide (CaOH) and triple antibiotic paste (TAP) with Bromelain against Enterococcus faecalis bacteria using the turbidity method.; Materials and Methods: Forty-four freshly extracted single-rooted teeth were collected and 6 mm sections were prepared. These samples were infected with E. faecalis bacteria (ATCC 29212), randomly divided into four groups. Negative control, CaOH paste, TAP, and Bromelain paste. After the removal of medicament, the optical density (OD) of the dentinal shavings was measured using a spectrophotometer.; Statistical Analysis: The Chi-square and Kruskal - Wallis ANOVA test were used. The confidence interval was set to 95% at 5% significance level.; Results: TAP showed less OD than CaOH2 paste which is statistically significant. (P = 0.0022). The OD reading of Bromelain paste was less than CaOH2 with P = 0.007. The OD of Bromelain and TAP were statistically insignificant (P = 0.095), indicating their comparable antibacterial efficacy.; Conclusions: The antimicrobial efficiency of Bromelain was found to be more effective than Ca (OH) 2 paste and comparable to TAP against E. faecalis.; (Copyright: © 2022 Journal of Conservative Dentistry.) |
| Competing Interests: | There are no conflicts of interest. |
| References: | J Conserv Dent. 2017 Sep-Oct;20(5):337-340. (PMID: 29386782); Indian J Dent Res. 2019 Nov-Dec;30(6):933-936. (PMID: 31939374); J Dent Res. 1987 Aug;66(8):1375-9. (PMID: 3114347); Int Endod J. 1992 Mar;25(2):97-106. (PMID: 1399059); J Endod. 2011 Sep;37(9):1287-9. (PMID: 21846550); J Int Oral Health. 2014 Sep;6(5):96-8. (PMID: 25395802); J Endod. 2004 May;30(5):315-20. (PMID: 15107642); Braz Dent J. 2009;20(1):32-6. (PMID: 19466228); Biomed Rep. 2016 Sep;5(3):283-288. (PMID: 27602208); Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2001 May;91(5):579-86. (PMID: 11346739); Braz Dent J. 2013;24(2):89-102. (PMID: 23780357); Ayu. 2014 Apr;35(2):113-8. (PMID: 25558153); Jundishapur J Nat Pharm Prod. 2012 Fall;7(4):140-3. (PMID: 24624172); Iran Endod J. 2014 Summer;9(3):195-8. (PMID: 25031593); Endod Dent Traumatol. 1990 Aug;6(4):142-9. (PMID: 2133305); Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2003 Nov;96(5):618-24. (PMID: 14600699); J Microbiol Immunol Infect. 2013 Apr;46(2):89-95. (PMID: 22572005); Quintessence Int. 2014 Sep;45(8):679-84. (PMID: 25019111); Cell Mol Life Sci. 2001 Aug;58(9):1234-45. (PMID: 11577981); J Endod. 2006 Feb;32(2):93-8. (PMID: 16427453); Int Endod J. 2011 Aug;44(8):697-730. (PMID: 21535021) |
| Contributed Indexing: | Keywords: Anti-microbial agent; bromelain; calcium hydroxide; in vitro; spectrophotometry |
| Entry Date(s): | Date Created: 20220620 Latest Revision: 20220716 |
| Update Code: | 20260130 |
| PubMed Central ID: | PMC9200187 |
| DOI: | 10.4103/jcd.jcd_461_21 |
| PMID: | 35722069 |
| Database: | MEDLINE |
Journal Article