Hypo-osmotic stress induces the epithelial alarmin IL-33 in the colonic barrier of ulcerative colitis.
| Title: | Hypo-osmotic stress induces the epithelial alarmin IL-33 in the colonic barrier of ulcerative colitis. |
|---|---|
| Authors: | Gundersen MD; Research Group of Gastroenterology and Nutrition, Institute of Clinical Medicine, UiT-The Arctic University of Norway, Tromsø, Norway. mdgundersen@gmail.com.; Larsen KB; Department of Medical Biology, Advanced Microscopy Core Facility, UiT-The Arctic University of Norway, Tromsø, Norway.; Johnsen KM; Research Group of Gastroenterology and Nutrition, Institute of Clinical Medicine, UiT-The Arctic University of Norway, Tromsø, Norway.; Division of Internal Medicine, Department of Gastroenterology, University Hospital of North Norway, Tromsø, Norway.; Goll R; Research Group of Gastroenterology and Nutrition, Institute of Clinical Medicine, UiT-The Arctic University of Norway, Tromsø, Norway.; Division of Internal Medicine, Department of Gastroenterology, University Hospital of North Norway, Tromsø, Norway.; Florholmen J; Research Group of Gastroenterology and Nutrition, Institute of Clinical Medicine, UiT-The Arctic University of Norway, Tromsø, Norway.; Haraldsen G; K.G. Jebsen Inflammation Research Centre, Institute of Clinical Medicine, Faculty of Medicine, University of Oslo, Oslo, Norway. |
| Source: | Scientific reports [Sci Rep] 2022 Jul 07; Vol. 12 (1), pp. 11550. Date of Electronic Publication: 2022 Jul 07. |
| Publication Type: | Journal Article; Research Support, Non-U.S. Gov't |
| Language: | English |
| Journal Info: | Publisher: Nature Publishing Group Country of Publication: England NLM ID: 101563288 Publication Model: Electronic Cited Medium: Internet ISSN: 2045-2322 (Electronic) Linking ISSN: 20452322 NLM ISO Abbreviation: Sci Rep Subsets: MEDLINE |
| Imprint Name(s): | Original Publication: London : Nature Publishing Group, copyright 2011- |
| MeSH Terms: | Alarmins*/metabolism ; Colitis, Ulcerative*/pathology ; Interleukin-33*/metabolism ; Osmotic Pressure*; Colon/pathology ; Cytokines/metabolism ; Inflammation/pathology ; Intestinal Mucosa/metabolism ; Humans |
| Abstract: | Epithelial alarmins are gaining interest as therapeutic targets for chronic inflammation. The nuclear alarmin interleukin-33 (IL-33) is upregulated in the colonic mucosa of acute ulcerative colitis (UC) and may represent an early instigator of the inflammatory cascade. However, it is not clear what signals drive the expression of IL-33 in the colonic mucosa, nor is the exact role of IL-33 elucidated. We established an ex vivo model using endoscopic colonic biopsies from healthy controls and UC patients. Colonic biopsies exposed to hypo-osmotic medium induced a strong nuclear IL-33 expression in colonic crypts in both healthy controls and UC biopsies. Mucosal IL33 mRNA was also significantly increased following hypo-osmotic stress in healthy controls compared to non-stimulated biopsies (fold change 3.9, p-value |
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| Substance Nomenclature: | 0 (Alarmins); 0 (Cytokines); 0 (IL33 protein, human); 0 (Interleukin-33) |
| Entry Date(s): | Date Created: 20220707 Date Completed: 20220711 Latest Revision: 20251031 |
| Update Code: | 20260130 |
| PubMed Central ID: | PMC9263100 |
| DOI: | 10.1038/s41598-022-15573-0 |
| PMID: | 35798804 |
| Database: | MEDLINE |
Journal Article; Research Support, Non-U.S. Gov't