Selective autophagy of RIPosomes maintains innate immune homeostasis during bacterial infection.
| Title: | Selective autophagy of RIPosomes maintains innate immune homeostasis during bacterial infection. |
|---|---|
| Authors: | Mehto S; Cell Biology and Infectious Diseases Unit, Department of Infectious Disease Biology, Institute of Life Sciences, Bhubaneswar, India.; Jena KK; Cell Biology and Infectious Diseases Unit, Department of Infectious Disease Biology, Institute of Life Sciences, Bhubaneswar, India.; Yadav R; Cell Biology and Infectious Diseases Unit, Department of Infectious Disease Biology, Institute of Life Sciences, Bhubaneswar, India.; Regional Centre for Biotechnology, NCR Biotech Science Cluster, Faridabad, India.; Priyadarsini S; Institute of Life Sciences, Bhubaneswar, India.; Samal P; Cell Biology and Infectious Diseases Unit, Department of Infectious Disease Biology, Institute of Life Sciences, Bhubaneswar, India.; Krishna S; Cell Biology and Infectious Diseases Unit, Department of Infectious Disease Biology, Institute of Life Sciences, Bhubaneswar, India.; Regional Centre for Biotechnology, NCR Biotech Science Cluster, Faridabad, India.; Dhar K; Cell Biology and Infectious Diseases Unit, Department of Infectious Disease Biology, Institute of Life Sciences, Bhubaneswar, India.; Regional Centre for Biotechnology, NCR Biotech Science Cluster, Faridabad, India.; Jain A; Centre for Cancer Cell Reprogramming, Institute of Clinical Medicine, Faculty of Medicine, University of Oslo, Oslo, Norway.; Department of Molecular Cell Biology, Institute for Cancer Research, Oslo University Hospital, Oslo, Norway.; Chauhan NR; Cell Biology and Infectious Diseases Unit, Department of Infectious Disease Biology, Institute of Life Sciences, Bhubaneswar, India.; Murmu KC; Epigenetic and Chromatin Biology Unit, Institute of Life Sciences, Bhubaneswar, India.; Bal R; Cell Biology and Infectious Diseases Unit, Department of Infectious Disease Biology, Institute of Life Sciences, Bhubaneswar, India.; School of Biotechnology, KIIT University, Bhubaneswar, India.; Sahu R; Cell Biology and Infectious Diseases Unit, Department of Infectious Disease Biology, Institute of Life Sciences, Bhubaneswar, India.; Regional Centre for Biotechnology, NCR Biotech Science Cluster, Faridabad, India.; Jaiswal P; Cell Biology and Infectious Diseases Unit, Department of Infectious Disease Biology, Institute of Life Sciences, Bhubaneswar, India.; Sahoo BS; Institute of Life Sciences, Bhubaneswar, India.; Patnaik S; School of Biotechnology, KIIT University, Bhubaneswar, India.; Kufer TA; Department of Immunology, Institute of Nutritional Medicine, University of Hohenheim, Stuttgart, Germany.; Rusten TE; Centre for Cancer Cell Reprogramming, Institute of Clinical Medicine, Faculty of Medicine, University of Oslo, Oslo, Norway.; Department of Molecular Cell Biology, Institute for Cancer Research, Oslo University Hospital, Oslo, Norway.; Chauhan S; Epigenetic and Chromatin Biology Unit, Institute of Life Sciences, Bhubaneswar, India.; Prasad P; Epigenetic and Chromatin Biology Unit, Institute of Life Sciences, Bhubaneswar, India.; Chauhan S; Cell Biology and Infectious Diseases Unit, Department of Infectious Disease Biology, Institute of Life Sciences, Bhubaneswar, India.; CSIR-Centre For Cellular And Molecular Biology (CCMB), Hyderabad, India. |
| Source: | The EMBO journal [EMBO J] 2022 Dec 01; Vol. 41 (23), pp. e111289. Date of Electronic Publication: 2022 Oct 11. |
| Publication Type: | Journal Article; Research Support, Non-U.S. Gov't |
| Language: | English |
| Journal Info: | Publisher: Nature Publishing Group Country of Publication: England NLM ID: 8208664 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1460-2075 (Electronic) Linking ISSN: 02614189 NLM ISO Abbreviation: EMBO J Subsets: MEDLINE |
| Imprint Name(s): | Publication: 2024- : [London] : Nature Publishing Group; Original Publication: Eynsham, Oxford, England : Published for the European Molecular Biology Organization by IRL Press, [c1982- |
| MeSH Terms: | NF-kappa B*/metabolism ; Bacterial Infections*; Mice ; Animals ; Mice, Inbred NOD ; Autophagy ; Immunity, Innate ; Homeostasis |
| Abstract: | The NOD1/2-RIPK2 is a key cytosolic signaling complex that activates NF-κB pro-inflammatory response against invading pathogens. However, uncontrolled NF-κB signaling can cause tissue damage leading to chronic diseases. The mechanisms by which the NODs-RIPK2-NF-κB innate immune axis is activated and resolved remain poorly understood. Here, we demonstrate that bacterial infection induces the formation of endogenous RIPK2 oligomers (RIPosomes) that are self-assembling entities that coat the bacteria to induce NF-κB response. Next, we show that autophagy proteins IRGM and p62/SQSTM1 physically interact with NOD1/2, RIPK2 and RIPosomes to promote their selective autophagy and limit NF-κB activation. IRGM suppresses RIPK2-dependent pro-inflammatory programs induced by Shigella and Salmonella. Consistently, the therapeutic inhibition of RIPK2 ameliorates Shigella infection- and DSS-induced gut inflammation in Irgm1 KO mice. This study identifies a unique mechanism where the innate immune proteins and autophagy machinery are recruited together to the bacteria for defense as well as for maintaining immune homeostasis.; (© 2022 The Authors. Published under the terms of the CC BY 4.0 license.) |
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| Grant Information: | United Kingdom WT_ Wellcome Trust |
| Contributed Indexing: | Keywords: Irgm1; NOD1/2-RIPK2-NF-κB; RIPosomes; autophagy; inflammation |
| Substance Nomenclature: | 0 (NF-kappa B) |
| Entry Date(s): | Date Created: 20221012 Date Completed: 20221202 Latest Revision: 20230621 |
| Update Code: | 20260130 |
| PubMed Central ID: | PMC9713718 |
| DOI: | 10.15252/embj.2022111289 |
| PMID: | 36221902 |
| Database: | MEDLINE |
Journal Article; Research Support, Non-U.S. Gov't