Bioactive Hydroxyapatite-Magnesium Phosphate Coatings Deposited by MAPLE for Preventing Infection and Promoting Orthopedic Implants Osteointegration.
| Title: | Bioactive Hydroxyapatite-Magnesium Phosphate Coatings Deposited by MAPLE for Preventing Infection and Promoting Orthopedic Implants Osteointegration. |
|---|---|
| Authors: | Florea DA; Department of Science and Engineering of Oxide Materials and Nanomaterials, Politehnica University of Bucharest, 011061 Bucharest, Romania.; Grumezescu V; National Institute for Lasers, Plasma and Radiation Physics, 077125 Magurele, Romania.; Bîrcă AC; Department of Science and Engineering of Oxide Materials and Nanomaterials, Politehnica University of Bucharest, 011061 Bucharest, Romania.; Vasile BȘ; Department of Science and Engineering of Oxide Materials and Nanomaterials, Politehnica University of Bucharest, 011061 Bucharest, Romania.; Iosif A; Department of Science and Engineering of Oxide Materials and Nanomaterials, Politehnica University of Bucharest, 011061 Bucharest, Romania.; Chircov C; Department of Science and Engineering of Oxide Materials and Nanomaterials, Politehnica University of Bucharest, 011061 Bucharest, Romania.; Stan MS; Research Institute of the University of Bucharest-ICUB, University of Bucharest, 050657 Bucharest, Romania.; Department of Biochemistry and Molecular Biology, Faculty of Biology, University of Bucharest, 050095 Bucharest, Romania.; Grumezescu AM; Department of Science and Engineering of Oxide Materials and Nanomaterials, Politehnica University of Bucharest, 011061 Bucharest, Romania.; Research Institute of the University of Bucharest-ICUB, University of Bucharest, 050657 Bucharest, Romania.; Academy of Romanian Scientists, Ilfov No. 3, 050044 Bucharest, Romania.; Andronescu E; Department of Science and Engineering of Oxide Materials and Nanomaterials, Politehnica University of Bucharest, 011061 Bucharest, Romania.; Academy of Romanian Scientists, Ilfov No. 3, 050044 Bucharest, Romania.; Chifiriuc MC; Academy of Romanian Scientists, Ilfov No. 3, 050044 Bucharest, Romania.; Department of Microbiology, Faculty of Biology, University of Bucharest, 060101 Bucharest, Romania.; The Romanian Academy, Calea Victoriei 25, District 1, 010071 Bucharest, Romania. |
| Source: | Materials (Basel, Switzerland) [Materials (Basel)] 2022 Oct 20; Vol. 15 (20). Date of Electronic Publication: 2022 Oct 20. |
| Publication Type: | Journal Article |
| Language: | English |
| Journal Info: | Publisher: MDPI Country of Publication: Switzerland NLM ID: 101555929 Publication Model: Electronic Cited Medium: Print ISSN: 1996-1944 (Print) Linking ISSN: 19961944 NLM ISO Abbreviation: Materials (Basel) Subsets: PubMed not MEDLINE |
| Imprint Name(s): | Publication: May 2010- : Basel, Switzerland : MDPI; Original Publication: Basel, Switzerland : Molecular Diversity Preservation International, 2008-2010. |
| Abstract: | In this study, we used the matrix-assisted pulsed laser evaporation (MAPLE) technique to obtain hydroxyapatite (Ca10(PO4)6(OH)2) and magnesium phosphate (Mg3(PO4)2) thin coatings containing bone morphogenetic protein (BMP4) for promoting implants osteointegration and further nebulized with the antibiotic ceftriaxone (CXF) to prevent peri-implant infections. The samples were characterized by X-ray diffraction (XRD), scanning electron microscopy (SEM), transmission electron microscopy (TEM), selected area electron diffraction (SAED), infrared microscopy (IRM) and Fourier-transform infrared spectroscopy (FT-IR). Furthermore, the antimicrobial properties were evaluated on Staphylococcus aureus biofilms and the cytocompatibility on the MC3T3-E1 cell line. The obtained results proved the potential of the obtained coatings for bone implant applications, providing a significant antimicrobial and antibiofilm effect, especially in the first 48 h, and cytocompatibility in relation to murine osteoblast cells. |
| Competing Interests: | The authors declare no conflict of interest. |
| References: | J Bone Jt Infect. 2016 Jul 19;1:34-41. (PMID: 28529851); J Bone Joint Surg Br. 2006 Jul;88(7):943-8. (PMID: 16799001); Materials (Basel). 2016 Sep 02;9(9):. (PMID: 28773871); Orthop Rev (Pavia). 2021 Mar 31;13(1):8794. (PMID: 33953887); Nanomedicine (Lond). 2015 Jul;10(14):2249-61. (PMID: 26119630); Nanomedicine (Lond). 2006 Oct;1(3):311-9. (PMID: 17716161); Biomaterials. 2020 Dec;261:120302. (PMID: 32932172); Int J Mol Sci. 2014 Jun 06;15(6):10150-68. (PMID: 24914764); J R Soc Interface. 2008 Oct 6;5(27):1137-58. (PMID: 18667387); Int J Antimicrob Agents. 2009 Apr;33(4):354-9. (PMID: 19097865); Mater Sci Eng C Mater Biol Appl. 2014 Feb 1;35:70-6. (PMID: 24411353); Clin Infect Dis. 2006 Oct 15;43(8):961-7. (PMID: 16983605); Med Oral Patol Oral Cir Bucal. 2011 Jan 01;16(1):e42-4. (PMID: 20526267); Regen Biomater. 2018 Aug;5(4):197-211. (PMID: 30094059); J Tissue Eng Regen Med. 2008 Jan;2(1):1-13. (PMID: 18293427); Clin Radiol. 2009 Oct;64(10):954-60. (PMID: 19748000); Biomed Mater Eng. 2016 May 12;27(1):87-99. (PMID: 27175470); Int Orthop. 1995;19(3):157-61. (PMID: 7558491); Int J Nanomedicine. 2018 Jul 11;13:4083-4092. (PMID: 30034234); Materials (Basel). 2020 Feb 07;13(3):. (PMID: 32046134); J R Soc Interface. 2010 Aug 6;7(49):1171-80. (PMID: 20181560); Materials (Basel). 2019 Feb 05;12(3):. (PMID: 30764574); Adv Healthc Mater. 2021 Nov;10(21):e2101051. (PMID: 34561956); Int J Mol Sci. 2021 Jan 18;22(2):. (PMID: 33477502); Proc Inst Mech Eng H. 2017 Sep;231(9):785-813. (PMID: 28486859); Int J Mol Sci. 2022 Jul 18;23(14):. (PMID: 35887261); Acta Biomater. 2011 Jun;7(6):2678-85. (PMID: 21324383); Nat Commun. 2021 Sep 16;12(1):5473. (PMID: 34531396); Am J Vet Res. 2007 Apr;68(4):370-8. (PMID: 17397291); Acta Biomater. 2011 Apr;7(4):1853-61. (PMID: 21147277); ANZ J Surg. 2007 Aug;77(8):626-31. (PMID: 17635273); J Bone Jt Infect. 2020 Jun 27;5(4):212-222. (PMID: 32670776); J R Soc Interface. 2019 Jul 26;16(156):20190259. (PMID: 31362615); Tissue Eng Part B Rev. 2015 Apr;21(2):218-30. (PMID: 25348836); Biomaterials. 2019 Oct;219:119366. (PMID: 31374482); Metallomics. 2020 Jun 24;12(6):916-934. (PMID: 32352129); Sci Rep. 2021 Mar 10;11(1):5539. (PMID: 33692436); Biomed Mater Eng. 2009;19(2-3):141-6. (PMID: 19581707); ISRN Orthop. 2011 Aug 07;2011:290851. (PMID: 24977058); Future Microbiol. 2014;9(8):987-1007. (PMID: 25302955); Clin Orthop Relat Res. 1996 Mar;(324):39-46. (PMID: 8595775); Orthopedics. 2009 Sep;32(9):. (PMID: 19751021) |
| Contributed Indexing: | Keywords: BMP4; MAPLE; Staphylococcus aureus biofilm; antibiotic-containing coatings; ceftriaxone; osteoblasts |
| Entry Date(s): | Date Created: 20221027 Latest Revision: 20240906 |
| Update Code: | 20260130 |
| PubMed Central ID: | PMC9609740 |
| DOI: | 10.3390/ma15207337 |
| PMID: | 36295401 |
| Database: | MEDLINE |
Journal Article