New Perspectives for Prosthetic Valve Endocarditis: Impact of Molecular Imaging by FISHseq Diagnostics.
| Title: | New Perspectives for Prosthetic Valve Endocarditis: Impact of Molecular Imaging by FISHseq Diagnostics. |
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| Authors: | Hajduczenia MM; Biofilmcenter, Institute for Microbiology, Infectious Diseases and Immunology, Charité-Universitätsmedizin Berlin, Berlin, Germany.; Department of Cardiology, Charité-Universitätsmedizin Berlin, Berlin, Germany.; Klefisch FR; Department of Internal Medicine and Intensive Care Medicine, Paulinen Hospital Berlin, Berlin, Germany.; Hopf AGM; Biofilmcenter, Institute for Microbiology, Infectious Diseases and Immunology, Charité-Universitätsmedizin Berlin, Berlin, Germany.; Grubitzsch H; Department of Cardiovascular Surgery, Charité-Universitätsmedizin Berlin, Berlin, Germany.; Stegemann MS; Department for Infectious Diseases and Respiratory Medicine, Charité-Universitätsmedizin Berlin, Berlin, Germany.; Pfäfflin F; Department for Infectious Diseases and Respiratory Medicine, Charité-Universitätsmedizin Berlin, Berlin, Germany.; Puhlmann B; Department of Anesthesiology and Intensive Care Medicine, Charité-Universitätsmedizin Berlin, Berlin, Germany.; Ocken M; Department of Anesthesiology and Intensive Care Medicine, Charité-Universitätsmedizin Berlin, Berlin, Germany.; Kretzler L; Clinical Trial Unit, Clinical Study Center, Berlin Institute of Health (BIH), Charité-Universitätsmedizin Berlin, Berlin, Germany.; von Schöning D; Department of Microbiology, Labor Berlin-Charité Vivantes GmbH, Berlin, Germany.; Falk V; Department of Cardiovascular Surgery, Charité-Universitätsmedizin Berlin, Berlin, Germany.; Moter A; Biofilmcenter, Institute for Microbiology, Infectious Diseases and Immunology, Charité-Universitätsmedizin Berlin, Berlin, Germany.; MoKi Analytics GmbH, Berlin, Germany.; Moter Diagnostics, Berlin, Germany.; Kikhney J; Biofilmcenter, Institute for Microbiology, Infectious Diseases and Immunology, Charité-Universitätsmedizin Berlin, Berlin, Germany.; MoKi Analytics GmbH, Berlin, Germany. |
| Source: | Clinical infectious diseases : an official publication of the Infectious Diseases Society of America [Clin Infect Dis] 2023 Mar 21; Vol. 76 (6), pp. 1050-1058. |
| Publication Type: | Journal Article; Research Support, Non-U.S. Gov't |
| Language: | English |
| Journal Info: | Publisher: Oxford University Press Country of Publication: United States NLM ID: 9203213 Publication Model: Print Cited Medium: Internet ISSN: 1537-6591 (Electronic) Linking ISSN: 10584838 NLM ISO Abbreviation: Clin Infect Dis Subsets: MEDLINE |
| Imprint Name(s): | Publication: Jan. 2011- : Oxford : Oxford University Press; Original Publication: Chicago, IL : The University of Chicago Press, c1992- |
| MeSH Terms: | Endocarditis, Bacterial*/microbiology ; Heart Valve Prosthesis*/adverse effects ; Prosthesis-Related Infections*/microbiology ; Endocarditis*/etiology; RNA, Ribosomal, 16S/genetics ; Humans ; Retrospective Studies ; In Situ Hybridization, Fluorescence ; Molecular Imaging |
| Abstract: | Background: The microbial etiology of prosthetic valve infective endocarditis (PVE) can be difficult to identify. Our aim was to investigate the benefit of molecular imaging technique fluorescence in situ hybridization (FISH) combined with 16S rRNA-gene polymerase chain reaction (PCR) and sequencing (FISHseq) for the analysis of infected prosthetic heart valves.; Methods: We retrospectively evaluated the diagnostic outcome of 113 prosthetic valves from 105 patients with suspected PVE, treated in 2003-2013 in the Department of Cardiac Surgery, Charité University Medicine Berlin. Each prosthetic valve underwent cultural diagnostics and was routinely examined by FISH combined with 16S rRNA gene PCR and sequencing. We compared classical microbiological culture outcomes (blood and valve cultures) with FISHseq results and evaluated the diagnostic impact of the molecular imaging technique.; Results: Conventional microbiological diagnostic alone turned out to be insufficient, as 67% of preoperative blood cultures were noninformative (negative, inconclusive, or not obtained) and 67% of valve cultures remained negative. FISHseq improved the conventional cultural diagnostic methods in PVE in 30% of the cases and increased diagnostic accuracy. Of the valve culture-negative PVE cases, FISHseq succeeded in identifying the causative pathogen in 35%.; Conclusions: FISHseq improves PVE diagnostics, complementing conventional cultural methods. In addition to species identification, FISH provides information about the severity of PVE and state of the pathogens (eg, stage of biofilm formation, activity, and localization on and within the prosthetic material). As a molecular imaging technique, FISHseq enables the unambiguous discrimination of skin flora as contaminant or infectious agent.; (© The Author(s) 2022. Published by Oxford University Press on behalf of Infectious Diseases Society of America.) |
| Competing Interests: | Potential conflicts of interest. A. M. and J. K. are employees of and hold shares in MoKi Analytics GmbH in addition to being employed by Charité, and A. M. is founder and head of the private practice Moter Diagnostics. D. v. S. reports support for attending meetings from Insmed Germany GmbH (participation fee NTM Summit Berlin 8.10.2022). A. M. reports German Federal Ministry of Research and Education (BMBF) grants 13N15824 (FIELD), 13GW0414A (PROCEED), and 13N15815 (TEAM) (to A. M. and J. K.; MoKi Analytics) and European Union ITN grant 814168 (GROWTH) (to A. M.; MoKi Analytics); honoraria for lectures by BioMerieux and Chiesi; aupport for invited talks at meetings by BÄMI, ECCMID, SGM, ERASMUS+, and REMMDI; patents planned, issued, or pending for “Control Preparation for Fish Methods in Microbiology” (PCT/EP2016/051630); a leadership or fiduciary role with Konsiliarlabor Tropheryma whipplei, appointed by Robert Koch-Institute; shares with MoKi Analytics GmbH, and private practice with Moter Diagnostics. J. K. reports German Federal Ministry of Research and Education (BMBF) grants 13N15824 (FIELD), 13GW0414A (PROCEED), and 13N15815 (TEAM) (to J. K.; MoKi Analytics) and European Union ITN grant 814168 (GROWTH) (to J. K.; MoKi Analytics); support for attending meetings and/or travel from the German Society of Hygiene and Microbiology (DGHM) (2022); patent I 07/137 (2008) Crystalline EGFR-matuzumab complex and matuzumab mimetics obtained thereof; a role as Vice Speaker of the Young German Society of Hygiene and Microbiology; and financial or nonfinancial interests as Chief Executive Officer of MoKi Analytics GmbH, a start-up of Charité, since 2018. V. F. is affiliated with the German Heart Center–Berlin, Charite–University Hospital Berlin, DZHK, ETH-Zurich (Department of Health, Science, and Technology and Trasl. Cardiovascular Technology). He also declares institutional financial activities in relation to the following: educational grants (including travel support), fees for lectures and speeches, fees for professional consultation, and research and study funds with the following commercial entities: Medtronic GmbH, Biotronik SE & Co, Abiomed GmbH, Abbott GmbH & Co, KG, Boston Scientific, Edwards Lifesciences, Berlin Heart, Novartis Pharma GmbH, JOTEC/CryoLife GmbH, LivaNova, and Zurich Heart. All other authors report no potential conflicts. All authors have submitted the ICMJE Form for Disclosure of Potential Conflicts of Interest. Conflicts that the editors consider relevant to the content of the manuscript have been disclosed. |
| References: | Clin Microbiol Infect. 2010 Jun;16(6):767-73. (PMID: 19694763); PLoS One. 2019 Aug 27;14(8):e0221786. (PMID: 31454398); Clin Microbiol Rev. 2001 Jan;14(1):177-207. (PMID: 11148009); J Thorac Cardiovasc Surg. 2010 Apr;139(4):887-93. (PMID: 19660339); Appl Environ Microbiol. 1990 Jun;56(6):1919-25. (PMID: 2200342); Curr Infect Dis Rep. 2010 Jul;12(4):244-52. (PMID: 21308538); JAMA. 2007 Mar 28;297(12):1354-61. (PMID: 17392239); Curr Opin Crit Care. 2013 Oct;19(5):461-4. (PMID: 23995121); F1000Res. 2018 May 29;7:. (PMID: 29904579); Appl Environ Microbiol. 1993 May;59(5):1354-60. (PMID: 7685999); Eur Heart J. 2019 Oct 14;40(39):3233-3236. (PMID: 31589743); Ann Thorac Surg. 2015 Jan;99(1):33-7. (PMID: 25442997); Int J Antimicrob Agents. 2008 Nov;32 Suppl 1:S51-9. (PMID: 18718741); Open Med (Wars). 2020 Jun 20;15(1):571-579. (PMID: 33336013); Heart. 2001 May;85(5):590-3. (PMID: 11303018); Heart Lung Circ. 2018 Mar;27(3):333-343. (PMID: 28764880); PLoS One. 2013 Dec 09;8(12):e82665. (PMID: 24349331); Cytometry. 1993;14(2):136-43. (PMID: 7679962); Eur J Clin Microbiol Infect Dis. 2006 Jun;25(6):365-8. (PMID: 16767483); Heart. 2005 Jul;91(7):954-9. (PMID: 15958370); Clin Infect Dis. 2000 Apr;30(4):633-8. (PMID: 10770721); Arch Intern Med. 2009 Mar 9;169(5):463-73. (PMID: 19273776); Eur Heart J. 2019 Oct 14;40(39):3222-3232. (PMID: 31504413); Medicine (Baltimore). 2015 Oct;94(43):e1816. (PMID: 26512582); Circulation. 2005 Mar 22;111(11):1352-4. (PMID: 15781748); Curr Infect Dis Rep. 2017 May;19(5):21. (PMID: 28401448); Interact Cardiovasc Thorac Surg. 2019 Nov 1;29(5):678-684. (PMID: 31274149); Eur J Clin Microbiol Infect Dis. 2018 Apr;37(4):765-771. (PMID: 29380224); Clin Infect Dis. 2023 Feb 8;76(3):e1484-e1491. (PMID: 35686318); Eur J Cardiothorac Surg. 2004 Dec;26(6):1104-11. (PMID: 15541970); Eur Heart J. 2015 Nov 21;36(44):3075-3128. (PMID: 26320109); FEMS Immunol Med Microbiol. 2006 Jun;47(1):1-13. (PMID: 16706783); BMC Infect Dis. 2016 Apr 12;16:146. (PMID: 27066823); J Clin Microbiol. 2017 Sep;55(9):2599-2608. (PMID: 28659319); J Clin Med. 2021 Feb 13;10(4):. (PMID: 33668597); EuroIntervention. 2021 Feb 19;16(14):1135-1140. (PMID: 32207407); J Clin Microbiol. 2018 Aug 27;56(9):. (PMID: 29899006); J Nucl Med. 2016 Nov;57(11):1669-1671. (PMID: 27261518) |
| Contributed Indexing: | Keywords: fluorescence in situ hybridization; implant infections; infectious endocarditis; molecular imaging; prosthetic valve endocarditis |
| Substance Nomenclature: | 0 (RNA, Ribosomal, 16S) |
| Entry Date(s): | Date Created: 20221101 Date Completed: 20230323 Latest Revision: 20230327 |
| Update Code: | 20260130 |
| PubMed Central ID: | PMC10029985 |
| DOI: | 10.1093/cid/ciac860 |
| PMID: | 36318608 |
| Database: | MEDLINE |
Journal Article; Research Support, Non-U.S. Gov't