Polyphasic evaluation and cytotoxic investigation of isolated cyanobacteria with an emphasis on potent activities of a Scytonema strain.
| Title: | Polyphasic evaluation and cytotoxic investigation of isolated cyanobacteria with an emphasis on potent activities of a Scytonema strain. |
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| Authors: | Ngo TT; Faculty of Biology, University of Science, Vietnam National University, Hanoi, Vietnam.; Nguyen BT; Faculty of Biology, University of Science, Vietnam National University, Hanoi, Vietnam.; Duong TA; Department of Biochemistry, Genetics, and Microbiology, Forestry and Agricultural Biotechnology Institute, University of Pretoria, Pretoria, South Africa.; Nguyen TT; Center for Life Sciences Research, Faculty of Biology, University of Science, Vietnam National University, Hanoi, Vietnam.; Nguyen TL; Faculty of Biology, University of Science, Vietnam National University, Hanoi, Vietnam.; Center for Life Sciences Research, Faculty of Biology, University of Science, Vietnam National University, Hanoi, Vietnam.; Kieu KT; Center for Life Sciences Research, Faculty of Biology, University of Science, Vietnam National University, Hanoi, Vietnam.; Do MT; Faculty of Biology, University of Science, Vietnam National University, Hanoi, Vietnam.; Nguyen SV; Faculty of Biology, University of Science, Vietnam National University, Hanoi, Vietnam.; Center for Life Sciences Research, Faculty of Biology, University of Science, Vietnam National University, Hanoi, Vietnam.; Thang ND; Faculty of Biology, University of Science, Vietnam National University, Hanoi, Vietnam.; Pham HTL; Faculty of Biology, University of Science, Vietnam National University, Hanoi, Vietnam.; The Key Laboratory of Enzyme and Protein Technology, University of Science, Vietnam National University, Hanoi, Vietnam. |
| Source: | Frontiers in microbiology [Front Microbiol] 2022 Oct 28; Vol. 13, pp. 1025755. Date of Electronic Publication: 2022 Oct 28 (Print Publication: 2022). |
| Publication Type: | Journal Article |
| Language: | English |
| Journal Info: | Publisher: Frontiers Research Foundation Country of Publication: Switzerland NLM ID: 101548977 Publication Model: eCollection Cited Medium: Print ISSN: 1664-302X (Print) Linking ISSN: 1664302X NLM ISO Abbreviation: Front Microbiol Subsets: PubMed not MEDLINE |
| Imprint Name(s): | Original Publication: Lausanne : Frontiers Research Foundation |
| Abstract: | Cyanobacteria are phototrophic organisms widely found in most types of natural habitats in the tropical regions of the world. In this study, we isolated and identified cyanobacterial strains from paddy soil in Hanoi (Vietnam) and investigated their cytotoxic activities. Five isolated cyanobacterial strains showed distinctive profiles of gene sequences (rRNA 16S and rbcL), phylogenetic placements, and morphological characteristics. Based on the polyphasic evaluation, they were classified as Scytonema bilaspurense NK13, Hapalosiphon welwitschii MD2411, Aulosira sp. XN1103, Desikacharya sp. NS2000, and Desmonostoc sp. NK1813. The cytotoxic screening revealed that the extract of strain Scytonema bilaspurense NK13 exhibited potent cytotoxic activities against four human cell lines of HeLa cells, OVCAR-8 cells, HaCaT cells, and HEK-293T cells, with IC50 values of 3.8, 34.2, 21.6, and 0.6 μg/mL, respectively. This is the first time a well-classified Scytonema strain from tropical habitat in Southeast Asia has been recognized as a potential producer of cytotoxic compounds.; (Copyright © 2022 Ngo, Nguyen, Duong, Nguyen, Nguyen, Kieu, Do, Nguyen, Thang and Pham.) |
| Competing Interests: | The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. |
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| Contributed Indexing: | Keywords: Aulosira; Desikacharya; Desmonostoc; Hapalosiphon; Scytonema; polyphasic approach |
| Entry Date(s): | Date Created: 20221117 Latest Revision: 20221118 |
| Update Code: | 20260130 |
| PubMed Central ID: | PMC9651001 |
| DOI: | 10.3389/fmicb.2022.1025755 |
| PMID: | 36386649 |
| Database: | MEDLINE |
Journal Article