Chemical Profile and Biological Activities of Fungal Strains Isolated from Piper nigrum Roots: Experimental and Computational Approaches.
| Title: | Chemical Profile and Biological Activities of Fungal Strains Isolated from Piper nigrum Roots: Experimental and Computational Approaches. |
|---|---|
| Authors: | Luyen ND; Institute of Natural Products Chemistry, Vietnam Academy of Science and Technology (VAST), 18 Hoang Quoc Viet, Caugiay, Hanoi, 10000, Vietnam.; Huong LM; Institute of Natural Products Chemistry, Vietnam Academy of Science and Technology (VAST), 18 Hoang Quoc Viet, Caugiay, Hanoi, 10000, Vietnam.; Ha NTT; Institute of Chemistry, Vietnam Academy of Science and Technology (VAST), 18 Hoang Quoc Viet, Caugiay, Hanoi, 10000, Vietnam.; Tra NT; Institute of Chemistry, Vietnam Academy of Science and Technology (VAST), 18 Hoang Quoc Viet, Caugiay, Hanoi, 10000, Vietnam.; Anh LTT; Institute of Chemistry, Vietnam Academy of Science and Technology (VAST), 18 Hoang Quoc Viet, Caugiay, Hanoi, 10000, Vietnam.; Tuyen NV; Institute of Chemistry, Vietnam Academy of Science and Technology (VAST), 18 Hoang Quoc Viet, Caugiay, Hanoi, 10000, Vietnam.; Posta K; Hungarian University of Agriculture and Life Sciences, Institute of Genetics and Biotechnology, Pater str 1., Godollo, H-2103, Hungary.; Son NT; Institute of Chemistry, Vietnam Academy of Science and Technology (VAST), 18 Hoang Quoc Viet, Caugiay, Hanoi, 10000, Vietnam.; Pham-The H; Hanoi University of Pharmacy, 13-15 Le Thanh Tong, Hanoi, 10000, Vietnam. or. |
| Source: | Chemistry & biodiversity [Chem Biodivers] 2023 Feb; Vol. 20 (2), pp. e202200456. Date of Electronic Publication: 2023 Jan 12. |
| Publication Type: | Journal Article |
| Language: | English |
| Journal Info: | Publisher: Verlag Helvetica Chimica Acta Country of Publication: Switzerland NLM ID: 101197449 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1612-1880 (Electronic) Linking ISSN: 16121872 NLM ISO Abbreviation: Chem Biodivers Subsets: MEDLINE |
| Imprint Name(s): | Original Publication: Zürich, Switzerland : Hoboken, NJ : Verlag Helvetica Chimica Acta ; Distributed in the USA by Wiley, c2004- |
| MeSH Terms: | Piper nigrum*; Plant Extracts/pharmacology ; Oleic Acid ; alpha-Glucosidases ; Molecular Docking Simulation ; Fungi ; Palmitic Acid ; Linoleic Acids |
| Abstract: | The current report describes the chemical investigation and biological activity of extracts produced by three fungal strains Fusarium oxysporum, Penicillium simplicissimum, and Fusarium proliferatum isolated from the roots of Piper nigrum L. growing in Vietnam. These fungi were namely determined by morphological and DNA analyses. GC/MS identification revealed that the EtOAc extracts of these fungi were associated with the presence of saturated and unsaturated fatty acids. These EtOAc extracts showed cytotoxicity towards cancer cell lines HepG2, inhibited various microbacterial organisms, especially fungus Aspergillus niger and yeast Candida albicans (the MIC values of 50-100 μg/mL). In α-glucosidase inhibitory assay, they induced the IC50 values of 1.00-2.53 μg/mL were better than positive control acarbose (169.80 μg/mL). The EtOAc extract of F. oxysporum also showed strong anti-inflammatory activity against NO production and PGE-2 level. Four major compounds linoleic acid (37.346 %), oleic acid (27.520 %), palmitic acid (25.547 %), and stearic acid (7.030 %) from the EtOAc extract of F. oxysporum were selective in molecular docking study, by which linoleic and oleic acids showed higher binding affinity towards α-glucosidase than palmitic and stearic acids. In subsequent docking assay with inducible nitric oxide synthase (iNOS), palmitic acid, oleic acid and linoleic acid could be moderate inhibitors.; (© 2022 Wiley-VHCA AG, Zurich, Switzerland.) |
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| Grant Information: | NDT.61.HU/19 Vietnam-Hungary; NCVCC07.02/21-21 Vietnam-Hungary |
| Contributed Indexing: | Keywords: Piper nigrum roots; biological evaluation; docking simulation; fungal strains |
| Substance Nomenclature: | 2UMI9U37CP (Oleic Acid); EC 3.2.1.20 (alpha-Glucosidases); 0 (Plant Extracts); 2V16EO95H1 (Palmitic Acid); 0 (Linoleic Acids) |
| Entry Date(s): | Date Created: 20221223 Date Completed: 20230224 Latest Revision: 20230224 |
| Update Code: | 20260130 |
| DOI: | 10.1002/cbdv.202200456 |
| PMID: | 36564341 |
| Database: | MEDLINE |
Journal Article