Synthesis and In Vitro Testing of YVO4:Eu3+@silica-NH-GDA-IgG Bio-Nano Complexes for Labelling MCF-7 Breast Cancer Cells.
| Title: | Synthesis and In Vitro Testing of YVO4:Eu3+@silica-NH-GDA-IgG Bio-Nano Complexes for Labelling MCF-7 Breast Cancer Cells. |
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| Authors: | Huong TT; Institute of Materials Science, Vietnam Academy of Science and Technology, 18 Hoang Quoc Viet, Hanoi 100000, Vietnam.; Vietnam Academy of Science and Technology, Graduate University of Science and Technology, 18 Hoang Quoc Viet, Hanoi 100000, Vietnam.; Vinh LT; Faculty of Basic Science, Hanoi University of Mining and Geology, 18 Pho Vien, Hanoi 100000, Vietnam.; Khuyen HT; Institute of Materials Science, Vietnam Academy of Science and Technology, 18 Hoang Quoc Viet, Hanoi 100000, Vietnam.; Vietnam Academy of Science and Technology, Graduate University of Science and Technology, 18 Hoang Quoc Viet, Hanoi 100000, Vietnam.; Tuyen LD; Faculty of Basic Science, Hanoi University of Mining and Geology, 18 Pho Vien, Hanoi 100000, Vietnam.; Van ND; Institute of Materials Science, Vietnam Academy of Science and Technology, 18 Hoang Quoc Viet, Hanoi 100000, Vietnam.; Thao DT; Institute of Biotechnology, Vietnam Academy of Science and Technology, 18 Hoang Quoc Viet, Cau Giay, Hanoi 100000, Vietnam.; Phuong HT; Department of Chemistry, Hanoi Medical University, 1 Ton That Tung, Hanoi 100000, Vietnam. |
| Source: | Molecules (Basel, Switzerland) [Molecules] 2022 Dec 29; Vol. 28 (1). Date of Electronic Publication: 2022 Dec 29. |
| Publication Type: | Journal Article |
| Language: | English |
| Journal Info: | Publisher: MDPI Country of Publication: Switzerland NLM ID: 100964009 Publication Model: Electronic Cited Medium: Internet ISSN: 1420-3049 (Electronic) Linking ISSN: 14203049 NLM ISO Abbreviation: Molecules Subsets: MEDLINE |
| Imprint Name(s): | Original Publication: Basel, Switzerland : MDPI, c1995- |
| MeSH Terms: | Silicon Dioxide* ; Breast Neoplasms*; Humans ; Female ; MCF-7 Cells ; In Vitro Techniques ; Immunoglobulin G |
| Abstract: | We present a visual tool and facile method to detect MCF-7 breast cancer cells by using YVO4:Eu3+@silica-NH-GDA-IgG bio-nanocomplexes. To obtain these complexes, YVO4:Eu3+ nanoparticles with a uniform size of 10-25 nm have been prepared firstly by the hydrothermal process, followed by surface functionalization to be bio-compatible and conjugated with cancer cells. The YVO4:Eu3+@silica-NH-GDA-IgG nanoparticles exhibited an enhanced red emission at 618 nm under an excitation wavelength of 355 nm and were strongly coupled with MCF-7 breast cancer cells via biological conjugation. These bio-nanocomplexes showed a superior sensitiveness for MCF-7 cancer cell labelling with a detection percentage as high as 82%, while no HEK-293A healthy cells were probed under the same conditions of in vitro experiments. In addition, the detection percentage of MCF-7 breast cancer cells increased significantly via the functionalization and conjugation of YVO4:Eu3+ nanoparticles. The experimental results demonstrated that the YVO4:Eu3+@silica-NH-GDA-IgG bio-nanocomplexes can be used as a promising labelling agent for biomedical imaging and diagnostics. |
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| Contributed Indexing: | Keywords: YVO4:Eu3+; bio-nanocomplexes; cancer cell labelling; labelling; nanoparticles |
| Substance Nomenclature: | 7631-86-9 (Silicon Dioxide); 0 (Immunoglobulin G) |
| Entry Date(s): | Date Created: 20230108 Date Completed: 20230110 Latest Revision: 20230111 |
| Update Code: | 20260130 |
| PubMed Central ID: | PMC9822125 |
| DOI: | 10.3390/molecules28010280 |
| PMID: | 36615474 |
| Database: | MEDLINE |
Journal Article