Development and laboratory evaluation of a competitive ELISA for serodiagnosis of Nipah and Hendra virus infection using recombinant Nipah glycoproteins and a monoclonal antibody.
| Title: | Development and laboratory evaluation of a competitive ELISA for serodiagnosis of Nipah and Hendra virus infection using recombinant Nipah glycoproteins and a monoclonal antibody. |
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| Authors: | Zhu W; National Centre for Foreign Animal Disease, Canadian Food Inspection Agency, Winnipeg, MB, Canada.; Pickering B; National Centre for Foreign Animal Disease, Canadian Food Inspection Agency, Winnipeg, MB, Canada.; Department of Medical Microbiology and Infectious Diseases, University of Manitoba, Winnipeg, MB, Canada.; Department of Veterinary Microbiology and Preventative Medicine, College of Veterinary Medicine, Iowa State University, Ames, IA, United States.; Smith G; National Centre for Foreign Animal Disease, Canadian Food Inspection Agency, Winnipeg, MB, Canada.; Pinette M; National Centre for Foreign Animal Disease, Canadian Food Inspection Agency, Winnipeg, MB, Canada.; Truong T; National Microbiology Laboratory, Public Health Agency of Canada, Winnipeg, MB, Canada.; Babiuk S; National Centre for Foreign Animal Disease, Canadian Food Inspection Agency, Winnipeg, MB, Canada.; Department of Immunology, University of Manitoba, Winnipeg, MB, Canada.; Kobasa D; Department of Medical Microbiology and Infectious Diseases, University of Manitoba, Winnipeg, MB, Canada.; National Microbiology Laboratory, Public Health Agency of Canada, Winnipeg, MB, Canada.; Banadyga L; Department of Medical Microbiology and Infectious Diseases, University of Manitoba, Winnipeg, MB, Canada.; National Microbiology Laboratory, Public Health Agency of Canada, Winnipeg, MB, Canada.; Yang M; National Centre for Foreign Animal Disease, Canadian Food Inspection Agency, Winnipeg, MB, Canada. |
| Source: | Frontiers in veterinary science [Front Vet Sci] 2023 Feb 01; Vol. 10, pp. 1120367. Date of Electronic Publication: 2023 Feb 01 (Print Publication: 2023). |
| Publication Type: | Journal Article |
| Language: | English |
| Journal Info: | Publisher: Frontiers Media S.A Country of Publication: Switzerland NLM ID: 101666658 Publication Model: eCollection Cited Medium: Print ISSN: 2297-1769 (Print) Linking ISSN: 22971769 NLM ISO Abbreviation: Front Vet Sci Subsets: PubMed not MEDLINE |
| Imprint Name(s): | Original Publication: Lausanne : Frontiers Media S.A., [2014]- |
| Abstract: | Introduction: Nipah virus (NiV) and Hendra virus (HeV), of the genus Henipavirus, family Paramyxoviridae, are classified as Risk Group 4 (RG4) pathogens that cause respiratory disease in pigs and acute/febrile encephalitis in humans with high mortality.; Methods: A competitive enzyme-linked immunosorbent assay (cELISA) using a monoclonal antibody (mAb) and recombinant NiV glycoprotein (G) was developed and laboratory evaluated using sera from experimental pigs, mini pigs and nonhuman primates. The test depends on competition between specific antibodies in positive sera and a virus-specific mAb for binding to NiV-G.; Results: Based on 1,199 negative and 71 NiV positive serum test results, the cutoff value was determined as 35% inhibition. The diagnostic sensitivity and specificity of the NiV cELISA was 98.58 and 99.92%, respectively. When testing sera from animals experimentally infected with NiV Malaysia, the cELISA detected antibodies from 14 days post-infection (dpi) and remained positive until the end of the experiment (28 dpi). Comparisons using the Kappa coefficient showed strong agreement (100%) between the cELISA and a plaque reduction neutralization test (PRNT).; Discussion: Because our cELISA is simpler, faster, and gives comparable or better results than PRNT, it would be an adequate screening test for suspect NiV and HeV cases, and it would also be useful for epidemiological surveillance of Henipavirus infections in different animal species without changing reagents.; (Copyright © 2023 Zhu, Pickering, Smith, Pinette, Truong, Babiuk, Kobasa, Banadyga and Yang.) |
| Competing Interests: | The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. |
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| Contributed Indexing: | Keywords: Hendra virus; Henipavirus; Nipah virus; competitive ELISA; monoclonal antibody; recombinant Nipah virus glycoprotein; serodiagnosis |
| Entry Date(s): | Date Created: 20230223 Latest Revision: 20230224 |
| Update Code: | 20260130 |
| PubMed Central ID: | PMC9929177 |
| DOI: | 10.3389/fvets.2023.1120367 |
| PMID: | 36816187 |
| Database: | MEDLINE |
Journal Article