Identification of the Vibrational Optical Coherence Tomography Corneal Cellular Peak.
| Title: | Identification of the Vibrational Optical Coherence Tomography Corneal Cellular Peak. |
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| Authors: | Daher ND; Cornea Service, Wills Eye Hospital, Sidney Kimmel Medical College at Thomas Jefferson University, Philadelphia, PA, USA.; Saad AS; Cornea Service, Wills Eye Hospital, Sidney Kimmel Medical College at Thomas Jefferson University, Philadelphia, PA, USA.; Jimenez HJ; Vickie and Jack Farber Vision Research Center, Wills Eye Hospital, Philadelphia, PA, USA.; Milman T; Pathology Department, Wills Eye Hospital, Sidney Kimmel Medical College at Thomas Jefferson University, Philadelphia, PA, USA.; Gonzalez-Martinez OG; Pathology Department, Wills Eye Hospital, Sidney Kimmel Medical College at Thomas Jefferson University, Philadelphia, PA, USA.; Deshmukh T; OptoVibronex, LLC., Bethlehem, PA, USA.; Pulido JS; Vickie and Jack Farber Vision Research Center, Wills Eye Hospital, Philadelphia, PA, USA.; Silver FH; OptoVibronex, LLC., Bethlehem, PA, USA.; Department of Pathology and Laboratory Medicine, Robert Wood Johnson Medical School, Rutgers, the State University of New Jersey, Piscataway, NJ, USA.; Benedetto DA; Center for Advanced Eye Care, Vero Beach, FL, USA.; Rapuano CJ; Cornea Service, Wills Eye Hospital, Sidney Kimmel Medical College at Thomas Jefferson University, Philadelphia, PA, USA.; Syed ZA; Cornea Service, Wills Eye Hospital, Sidney Kimmel Medical College at Thomas Jefferson University, Philadelphia, PA, USA. |
| Source: | Translational vision science & technology [Transl Vis Sci Technol] 2023 Apr 03; Vol. 12 (4), pp. 11. |
| Publication Type: | Journal Article; Research Support, Non-U.S. Gov't |
| Language: | English |
| Journal Info: | Publisher: Association for Research in Vision and Ophthalmology Country of Publication: United States NLM ID: 101595919 Publication Model: Print Cited Medium: Internet ISSN: 2164-2591 (Electronic) Linking ISSN: 21642591 NLM ISO Abbreviation: Transl Vis Sci Technol Subsets: MEDLINE |
| Imprint Name(s): | Original Publication: Rockville, Md. : Association for Research in Vision and Ophthalmology |
| MeSH Terms: | Tomography, Optical Coherence*/methods ; Cornea*/diagnostic imaging; Humans ; Cell Count ; Tissue Donors |
| Abstract: | Purpose: Our team previously identified the presence of five corneal resonant frequency (RF) peaks in healthy volunteers using vibrational optical coherence tomography (VOCT). Prior studies have suggested that the ≤100 Hz RF peak represents the cellular element of tissue. The aim of this study was to confirm that this peak reflects the human corneal cellular component using VOCT and histological analysis.; Methods: Two human research globes were obtained from the same donor, and VOCT measurements were collected from the full-thickness corneas. A microkeratome was then used to create serial-free corneal caps from each cornea, with VOCT performed on the residual stromal bed after each excision. All lamellar sections from both globes were sent for histological analysis to determine cellularity. Cell counts on the specimens were performed by two independent observers.; Results: The average of the normalized ≤100 Hz peak values before lamellar sectioning was significantly higher than the average of this peak values after the first, second, and third cuts (P = 0.023), which was 33.9% less than before any cuts. The cell count values in the first slice were significantly higher than the average cell count values of the three deeper slices (P < 0.001), and the cell count dropped 84.4% after the first slice was removed.; Conclusions: The findings of this study suggest that the ≤100 Hz corneal peak identified by VOCT corresponds to the cellular component of the cornea.; Translational Relevance: This work furthers our understanding of the origin of the corneal ≤100 Hz peak identified using VOCT. |
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| Entry Date(s): | Date Created: 20230412 Date Completed: 20230414 Latest Revision: 20230517 |
| Update Code: | 20260130 |
| PubMed Central ID: | PMC10103735 |
| DOI: | 10.1167/tvst.12.4.11 |
| PMID: | 37043335 |
| Database: | MEDLINE |
Journal Article; Research Support, Non-U.S. Gov't