Synergistic Antimicrobial Activity of Magnetite and Vancomycin-Loaded Mesoporous Silica Embedded in Alginate Films.
| Title: | Synergistic Antimicrobial Activity of Magnetite and Vancomycin-Loaded Mesoporous Silica Embedded in Alginate Films. |
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| Authors: | Dolete G; Department of Science and Engineering of Oxide Materials and Nanomaterials, Faculty of Chemical Engineering and Biotechnologies, University Politehnica of Bucharest, Gh. Polizu 1-7, 011061 Bucharest, Romania.; National Center for Micro and Nanomaterials, University Politehnica of Bucharest, Splaiul Independenței 313, 060042 Bucharest, Romania.; Ilie CI; Department of Science and Engineering of Oxide Materials and Nanomaterials, Faculty of Chemical Engineering and Biotechnologies, University Politehnica of Bucharest, Gh. Polizu 1-7, 011061 Bucharest, Romania.; National Center for Micro and Nanomaterials, University Politehnica of Bucharest, Splaiul Independenței 313, 060042 Bucharest, Romania.; Chircov C; Department of Science and Engineering of Oxide Materials and Nanomaterials, Faculty of Chemical Engineering and Biotechnologies, University Politehnica of Bucharest, Gh. Polizu 1-7, 011061 Bucharest, Romania.; National Center for Micro and Nanomaterials, University Politehnica of Bucharest, Splaiul Independenței 313, 060042 Bucharest, Romania.; Purcăreanu B; Department of Science and Engineering of Oxide Materials and Nanomaterials, Faculty of Chemical Engineering and Biotechnologies, University Politehnica of Bucharest, Gh. Polizu 1-7, 011061 Bucharest, Romania.; BIOTEHNOS SA, Gorunului Street 3-5, 075100 Otopeni, Romania.; Motelica L; Department of Science and Engineering of Oxide Materials and Nanomaterials, Faculty of Chemical Engineering and Biotechnologies, University Politehnica of Bucharest, Gh. Polizu 1-7, 011061 Bucharest, Romania.; National Center for Micro and Nanomaterials, University Politehnica of Bucharest, Splaiul Independenței 313, 060042 Bucharest, Romania.; Moroșan A; Department of Organic Chemistry 'Costin Nenițescu', Faculty of Chemical Engineering and Biotechnologies, University Politehnica of Bucharest, Gh. Polizu 1-7, 011061 Bucharest, Romania.; Oprea OC; National Center for Micro and Nanomaterials, University Politehnica of Bucharest, Splaiul Independenței 313, 060042 Bucharest, Romania.; Department of Inorganic Chemistry, Physical Chemistry, and Electrochemistry, Faculty of Chemical Engineering and Biotechnologies, University Politehnica of Bucharest, Gh. Polizu 1-7, 011061 Bucharest, Romania.; Academy of Romanian Scientists, Ilfov Street 3, 050044 Bucharest, Romania.; Ficai D; Department of Science and Engineering of Oxide Materials and Nanomaterials, Faculty of Chemical Engineering and Biotechnologies, University Politehnica of Bucharest, Gh. Polizu 1-7, 011061 Bucharest, Romania.; National Center for Micro and Nanomaterials, University Politehnica of Bucharest, Splaiul Independenței 313, 060042 Bucharest, Romania.; Academy of Romanian Scientists, Ilfov Street 3, 050044 Bucharest, Romania.; Andronescu E; Department of Science and Engineering of Oxide Materials and Nanomaterials, Faculty of Chemical Engineering and Biotechnologies, University Politehnica of Bucharest, Gh. Polizu 1-7, 011061 Bucharest, Romania.; National Center for Micro and Nanomaterials, University Politehnica of Bucharest, Splaiul Independenței 313, 060042 Bucharest, Romania.; Academy of Romanian Scientists, Ilfov Street 3, 050044 Bucharest, Romania.; Dițu LM; Department of Microbiology and Immunology, Faculty of Biology, University of Bucharest, 1-3 Aleea Portocalelor, 060101 Bucharest, Romania.; Research Institute of the University of Bucharest, 91-95 Splaiul Independenței, 050095 Bucharest, Romania. |
| Source: | Gels (Basel, Switzerland) [Gels] 2023 Apr 02; Vol. 9 (4). Date of Electronic Publication: 2023 Apr 02. |
| Publication Type: | Journal Article |
| Language: | English |
| Journal Info: | Publisher: MDPI AG Country of Publication: Switzerland NLM ID: 101696925 Publication Model: Electronic Cited Medium: Internet ISSN: 2310-2861 (Electronic) Linking ISSN: 23102861 NLM ISO Abbreviation: Gels Subsets: PubMed not MEDLINE |
| Imprint Name(s): | Original Publication: Basel, Switzerland : MDPI AG, [2015]- |
| Abstract: | The aim of the present study was to obtain a hydrogel-based film as a carrier for the sustained and controlled release of vancomycin, an antibiotic commonly used in various types of infections. Considering the high-water solubility of vancomycin (>50 mg/mL) and the aqueous medium underlying the exudates, a prolonged release of vancomycin from an MCM-41 carrier was sought. The present work focused on the synthesis of malic acid coated magnetite (Fe3O4/malic) by co-precipitation, synthesis of MCM-41 by a sol-gel method and loading of MCM-41 with vancomycin, and their use in alginate films for wound dressing. The nanoparticles obtained were physically mixed and embedded in the alginate gel. Prior to incorporation, the nanoparticles were characterized by XRD, FT-IR and FT-Raman spectroscopy, TGA-DSC and DLS. The films were prepared by a simple casting method and were further cross-linked and examined for possible heterogeneities by means of FT-IR microscopy and SEM. The degree of swelling and the water vapor transmission rate were determined, considering their potential use as wound dressings. The obtained films show morpho-structural homogeneity, sustained release over 48 h and a strong synergistic enhancement of the antimicrobial activity as a consequence of the hybrid nature of these films. The antimicrobial efficacy was tested against S. aureus, two strains of E. faecalis (including vancomycin-resistant Enterococcus, VRE) and C. albicans. The incorporation of magnetite was also considered as an external triggering component in case the films were used as a magneto-responsive smart dressing to stimulate vancomycin diffusion. |
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| Contributed Indexing: | Keywords: antimicrobial wound dressings; dual release mechanism; hybrid nanostructured alginate films; magnetite; mesoporous silica; synergistic activity |
| Entry Date(s): | Date Created: 20230427 Latest Revision: 20230430 |
| Update Code: | 20260130 |
| PubMed Central ID: | PMC10137406 |
| DOI: | 10.3390/gels9040295 |
| PMID: | 37102906 |
| Database: | MEDLINE |
Journal Article