Benzimidazole Resistance in Cattle: The First Report of the Presence of F200Y Mutation in Cooperia in Ecuador.
| Title: | Benzimidazole Resistance in Cattle: The First Report of the Presence of F200Y Mutation in Cooperia in Ecuador. |
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| Authors: | Vinueza P; Escuela Superior Politécnica de Chimborazo, Facultad de Ciencias Pecuarias, Carrera de Medicina Veterinaria, Riobamba 060106, Ecuador.; Laboratory of Parasitology, Faculty of Veterinary Medicine, Ghent University, Salisburylaan 133-B, 9820 Merelbeke, Belgium.; Calispa M; UCLouvain, Earth al Life Institute ELIE, ELI Croix du Sud 2L/L7.05.05, 1348 Louvain-la-Neuve, Belgium.; Condolo L; Escuela Superior Politécnica de Chimborazo, Facultad de Ciencias Pecuarias, Carrera de Medicina Veterinaria, Riobamba 060106, Ecuador.; Toalombo P; Escuela Superior Politécnica de Chimborazo, Facultad de Ciencias Pecuarias, Carrera de Medicina Veterinaria, Riobamba 060106, Ecuador.; Geldhof P; Laboratory of Parasitology, Faculty of Veterinary Medicine, Ghent University, Salisburylaan 133-B, 9820 Merelbeke, Belgium. |
| Source: | Veterinary sciences [Vet Sci] 2023 May 28; Vol. 10 (6). Date of Electronic Publication: 2023 May 28. |
| Publication Type: | Journal Article |
| Language: | English |
| Journal Info: | Publisher: MDPI Country of Publication: Switzerland NLM ID: 101680127 Publication Model: Electronic Cited Medium: Internet ISSN: 2306-7381 (Electronic) Linking ISSN: 23067381 NLM ISO Abbreviation: Vet Sci Subsets: PubMed not MEDLINE |
| Imprint Name(s): | Original Publication: Basel : MDPI, [2014]- |
| Abstract: | Anthelmintic resistance among GINs in cattle is a worldwide issue. Identifying the early signs of anthelmintic resistance (AR) is necessary to sustainably manage bovine parasitic infections. This study aimed to evaluate the resistance status of bovine parasitic nematodes against FBZ on a farm with a known history of broad-spectrum anthelmintic usage in Ecuador. FBZ efficacy was analyzed using a fecal egg count reduction test (FECR test) and β-tubulin 1 mutation identification in Cooperia spp., the dominant nematode parasite identified before and after treatment. According to the FECR test, the nematode population was susceptible to FBZ. After amplifying and cloning the β-tubulin 1 of Cooperia spp., an F200Y mutation was found in 43% of the pooled larva coproculture after treatment. This study reports, for the first time, the presence of F200Y resistance-conferring mutation in Cooperia spp. in Ecuador. Although the nematode population was phenotypically susceptible to FBZ, the presence of F200Y suggests the existence of resistance in the early stages. Our findings highlight the need to implement alternative control strategies for parasitic infections besides broad-spectrum anthelmintics. |
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| Contributed Indexing: | Keywords: Cooperia spp.; Ecuador; anthelmintic; benzimidazole; molecular |
| Entry Date(s): | Date Created: 20230627 Latest Revision: 20230701 |
| Update Code: | 20260130 |
| PubMed Central ID: | PMC10305122 |
| DOI: | 10.3390/vetsci10060378 |
| PMID: | 37368764 |
| Database: | MEDLINE |
Journal Article