Bifurcate evolution of quinone synthetases in basidiomycetes.
| Title: | Bifurcate evolution of quinone synthetases in basidiomycetes. |
|---|---|
| Authors: | Seibold PS; Institute of Pharmacy, Department Pharmaceutical Microbiology, Friedrich Schiller University Jena, Winzerlaer Strasse 2, 07745, Jena, Germany.; Department Pharmaceutical Microbiology, Leibniz Institute for Natural Product Research and Infection Biology - Hans Knöll Institute, Winzerlaer Strasse 2, 07745, Jena, Germany.; Lawrinowitz S; Institute of Pharmacy, Department Pharmaceutical Microbiology, Friedrich Schiller University Jena, Winzerlaer Strasse 2, 07745, Jena, Germany.; Department Pharmaceutical Microbiology, Leibniz Institute for Natural Product Research and Infection Biology - Hans Knöll Institute, Winzerlaer Strasse 2, 07745, Jena, Germany.; Raztsou I; Institute of Organic Chemistry and Macromolecular Chemistry, Friedrich-Schiller-Universität Jena, Humboldtstrasse 10, 07743, Jena, Germany.; Gressler M; Institute of Pharmacy, Department Pharmaceutical Microbiology, Friedrich Schiller University Jena, Winzerlaer Strasse 2, 07745, Jena, Germany.; Department Pharmaceutical Microbiology, Leibniz Institute for Natural Product Research and Infection Biology - Hans Knöll Institute, Winzerlaer Strasse 2, 07745, Jena, Germany.; Arndt HD; Institute of Organic Chemistry and Macromolecular Chemistry, Friedrich-Schiller-Universität Jena, Humboldtstrasse 10, 07743, Jena, Germany.; Stallforth P; Department Paleobiotechnology, Leibniz Institute for Natural Product Research and Infection Biology - Hans Knöll Institute, Winzerlaer Strasse 2, 07745, Jena, Germany.; Hoffmeister D; Institute of Pharmacy, Department Pharmaceutical Microbiology, Friedrich Schiller University Jena, Winzerlaer Strasse 2, 07745, Jena, Germany. dirk.hoffmeister@leibniz-hki.de.; Department Pharmaceutical Microbiology, Leibniz Institute for Natural Product Research and Infection Biology - Hans Knöll Institute, Winzerlaer Strasse 2, 07745, Jena, Germany. dirk.hoffmeister@leibniz-hki.de. |
| Source: | Fungal biology and biotechnology [Fungal Biol Biotechnol] 2023 Jul 03; Vol. 10 (1), pp. 14. Date of Electronic Publication: 2023 Jul 03. |
| Publication Type: | Journal Article |
| Language: | English |
| Journal Info: | Publisher: BioMed Central Country of Publication: England NLM ID: 101655873 Publication Model: Electronic Cited Medium: Internet ISSN: 2054-3085 (Electronic) Linking ISSN: 20543085 NLM ISO Abbreviation: Fungal Biol Biotechnol Subsets: PubMed not MEDLINE |
| Imprint Name(s): | Original Publication: London : BioMed Central, [2014]- |
| Abstract: | Background: The terphenylquinones represent an ecologically remarkable class of basidiomycete natural products as they serve as central precursors of pigments and compounds that impact on microbial consortia by modulating bacterial biofilms and motility. This study addressed the phylogenetic origin of the quinone synthetases that assemble the key terphenylquinones polyporic acid and atromentin.; Results: The activity of the Hapalopilus rutilans synthetases HapA1, HapA2 and of Psilocybe cubensis PpaA1 were reconstituted in Aspergilli. Liquid chromatography and mass spectrometry of the culture extracts identified all three enzymes as polyporic acid synthetases. PpaA1 is unique in that it features a C-terminal, yet catalytically inactive dioxygenase domain. Combined with bioinformatics to reconstruct the phylogeny, our results demonstrate that basidiomycete polyporic acid and atromentin synthetases evolved independently, although they share an identical catalytic mechanism and release structurally very closely related products. A targeted amino acid replacement in the substrate binding pocket of the adenylation domains resulted in bifunctional synthetases producing both polyporic acid and atromentin.; Conclusions: Our results imply that quinone synthetases evolved twice independently in basidiomycetes, depending on the aromatic α-keto acid substrate. Furthermore, key amino acid residues for substrate specificity were identified and changed which led to a relaxed substrate profile. Therefore, our work lays the foundation for future targeted enzyme engineering.; (© 2023. The Author(s).) |
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| Grant Information: | R01 GM129325 United States GM NIGMS NIH HHS; EXC 2051 Deutsche Forschungsgemeinschaft |
| Contributed Indexing: | Keywords: Atromentin; Basidiomycota; Hapalopilus; Natural products; Polyporic acid; Psilocybe; Quinone synthetase; Terana |
| Entry Date(s): | Date Created: 20230703 Latest Revision: 20240706 |
| Update Code: | 20260130 |
| PubMed Central ID: | PMC10316625 |
| DOI: | 10.1186/s40694-023-00162-1 |
| PMID: | 37400920 |
| Database: | MEDLINE |
Journal Article