A Microfluidic Approach for Synthesis of Silver Nanoparticles as a Potential Antimicrobial Agent in Alginate-Hyaluronic Acid-Based Wound Dressings.
| Title: | A Microfluidic Approach for Synthesis of Silver Nanoparticles as a Potential Antimicrobial Agent in Alginate-Hyaluronic Acid-Based Wound Dressings. |
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| Authors: | Bîrcă AC; Department of Science and Engineering of Oxide Materials and Nanomaterials, Politehnica University of Bucharest, 011061 Bucharest, Romania.; Gherasim O; Lasers Department, National Institute for Lasers, Plasma and Radiation Physics, 409 Atomistilor Street, 077125 Magurele, Romania.; Niculescu AG; Department of Science and Engineering of Oxide Materials and Nanomaterials, Politehnica University of Bucharest, 011061 Bucharest, Romania.; Research Institute of the University of Bucharest-ICUB, University of Bucharest, 050657 Bucharest, Romania.; Grumezescu AM; Department of Science and Engineering of Oxide Materials and Nanomaterials, Politehnica University of Bucharest, 011061 Bucharest, Romania.; Research Institute of the University of Bucharest-ICUB, University of Bucharest, 050657 Bucharest, Romania.; Academy of Romanian Scientists, Ilfov No. 3, 050044 Bucharest, Romania.; Neacșu IA; Department of Science and Engineering of Oxide Materials and Nanomaterials, Politehnica University of Bucharest, 011061 Bucharest, Romania.; Chircov C; Department of Science and Engineering of Oxide Materials and Nanomaterials, Politehnica University of Bucharest, 011061 Bucharest, Romania.; Vasile BȘ; Department of Science and Engineering of Oxide Materials and Nanomaterials, Politehnica University of Bucharest, 011061 Bucharest, Romania.; Oprea OC; Department of Inorganic Chemistry, Physical Chemistry and Electrochemistry, University Politehnica of Bucharest, 1-7 Polizu St., 011061 Bucharest, Romania.; Andronescu E; Department of Science and Engineering of Oxide Materials and Nanomaterials, Politehnica University of Bucharest, 011061 Bucharest, Romania.; Academy of Romanian Scientists, Ilfov No. 3, 050044 Bucharest, Romania.; Stan MS; Research Institute of the University of Bucharest-ICUB, University of Bucharest, 050657 Bucharest, Romania.; Curuțiu C; Research Institute of the University of Bucharest-ICUB, University of Bucharest, 050657 Bucharest, Romania.; Department of Microbiology and Immunology, Faculty of Biology, University of Bucharest, 077206 Bucharest, Romania.; Dițu LM; Research Institute of the University of Bucharest-ICUB, University of Bucharest, 050657 Bucharest, Romania.; Department of Microbiology and Immunology, Faculty of Biology, University of Bucharest, 077206 Bucharest, Romania.; Holban AM; Research Institute of the University of Bucharest-ICUB, University of Bucharest, 050657 Bucharest, Romania.; Department of Microbiology and Immunology, Faculty of Biology, University of Bucharest, 077206 Bucharest, Romania. |
| Source: | International journal of molecular sciences [Int J Mol Sci] 2023 Jul 14; Vol. 24 (14). Date of Electronic Publication: 2023 Jul 14. |
| Publication Type: | Journal Article |
| Language: | English |
| Journal Info: | Publisher: MDPI Country of Publication: Switzerland NLM ID: 101092791 Publication Model: Electronic Cited Medium: Internet ISSN: 1422-0067 (Electronic) Linking ISSN: 14220067 NLM ISO Abbreviation: Int J Mol Sci Subsets: MEDLINE |
| Imprint Name(s): | Original Publication: Basel, Switzerland : MDPI, [2000- |
| MeSH Terms: | Metal Nanoparticles*/chemistry ; Anti-Infective Agents*/pharmacology; Hyaluronic Acid/chemistry ; Silver/pharmacology ; Silver/chemistry ; Alginates/chemistry ; Anti-Bacterial Agents/pharmacology ; Anti-Bacterial Agents/chemistry ; Microfluidics ; Spectroscopy, Fourier Transform Infrared ; Bandages |
| Abstract: | The recognized antimicrobial activity of silver nanoparticles is a well-studied property, especially when designing and developing biomaterials with medical applications. As biological activity is closely related to the physicochemical characteristics of a material, aspects such as particle morphology and dimension should be considered. Microfluidic systems in continuous flow represent a promising method to control the size, shape, and size distribution of synthesized nanoparticles. Moreover, using microfluidics widens the synthesis options by creating and controlling parameters that are otherwise difficult to maintain in conventional batch procedures. This study used a microfluidic platform with a cross-shape design as an innovative method for synthesizing silver nanoparticles and varied the precursor concentration and the purging speed as experimental parameters. The compositional and microstructural characterization of the obtained samples was carried out by X-ray diffraction (XRD), scanning electron microscopy (SEM), transmission electron microscopy (TEM), and dynamic light scattering (DLS). Four formulations of alginate-based hydrogels with the addition of hyaluronic acid and silver nanoparticles were obtained to highlight the antimicrobial activity of silver nanoparticles and the efficiency of such a composite in wound treatment. The porous structure, swelling capacity, and biological properties were evaluated through physicochemical analysis (FT-IR and SEM) and through contact with prokaryotic and eukaryotic cells. The results of the physicochemical and biological investigations revealed desirable characteristics for performant wound dressings (i.e., biocompatibility, appropriate porous structure, swelling rate, and degradation rate, ability to inhibit biofilm formation, and cell growth stimulation capacity), and the obtained materials are thus recommended for treating chronic and infected wounds. |
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| Contributed Indexing: | Keywords: alginate-hyaluronic acid; microfluidic platform; silver nanoparticles |
| Substance Nomenclature: | 9004-61-9 (Hyaluronic Acid); 3M4G523W1G (Silver); 0 (Alginates); 0 (Anti-Infective Agents); 0 (Anti-Bacterial Agents) |
| Entry Date(s): | Date Created: 20230729 Date Completed: 20230731 Latest Revision: 20240922 |
| Update Code: | 20260130 |
| PubMed Central ID: | PMC10380883 |
| DOI: | 10.3390/ijms241411466 |
| PMID: | 37511219 |
| Database: | MEDLINE |
Journal Article