NAK-associated protein 1/NAP1 activates TBK1 to ensure accurate mitosis and cytokinesis.
| Title: | NAK-associated protein 1/NAP1 activates TBK1 to ensure accurate mitosis and cytokinesis. |
|---|---|
| Authors: | Paul S; Graduate Program in Biomedical and Veterinary Sciences, Virginia-Maryland College of Veterinary Medicine, Blacksburg, VA, USA.; Sarraf SA; Biochemistry Section, National Institutes of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, MD, USA.; Nam KH; Cell Biology Program, Sloan Kettering Institute, Memorial Sloan Kettering Cancer Center, New York, NY, USA.; Zavar L; School of Neuroscience, Virginia Polytechnic Institute and State University, Blacksburg, VA, USA.; DeFoor N; School of Neuroscience, Virginia Polytechnic Institute and State University, Blacksburg, VA, USA.; Biswas SR; Translational Biology, Medicine, and Health Graduate Program, Virginia Polytechnic Institute and State University, Roanoke, VA, USA.; Fritsch LE; Translational Biology, Medicine, and Health Graduate Program, Virginia Polytechnic Institute and State University, Roanoke, VA, USA.; Yaron TM; Meyer Cancer Center, Weill Cornell Medicine, New York, NY, USA.; Englander Institute for Precision Medicine, Institute for Computational Biomedicine, Weill Cornell Medicine, New York, NY, USA.; Johnson JL; Meyer Cancer Center, Weill Cornell Medicine, New York, NY, USA.; Huntsman EM; Meyer Cancer Center, Weill Cornell Medicine, New York, NY, USA.; Englander Institute for Precision Medicine, Institute for Computational Biomedicine, Weill Cornell Medicine, New York, NY, USA.; Cantley LC; Meyer Cancer Center, Weill Cornell Medicine, New York, NY, USA.; Department of Cell Biology, Harvard Medical School, Boston, MA, USA.; Dana-Farber Cancer Institute, Harvard Medical School, Boston, MA, USA.; Ordureau A; Cell Biology Program, Sloan Kettering Institute, Memorial Sloan Kettering Cancer Center, New York, NY, USA.; Pickrell AM; School of Neuroscience, Virginia Polytechnic Institute and State University, Blacksburg, VA, USA. |
| Source: | The Journal of cell biology [J Cell Biol] 2024 Feb 05; Vol. 223 (2). Date of Electronic Publication: 2023 Dec 07. |
| Publication Type: | Journal Article; Research Support, N.I.H., Extramural |
| Language: | English |
| Journal Info: | Publisher: Rockefeller University Press Country of Publication: United States NLM ID: 0375356 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1540-8140 (Electronic) Linking ISSN: 00219525 NLM ISO Abbreviation: J Cell Biol Subsets: MEDLINE |
| Imprint Name(s): | Original Publication: New York : Rockefeller University Press |
| MeSH Terms: | Adaptor Proteins, Signal Transducing*/genetics ; Adaptor Proteins, Signal Transducing*/metabolism ; Protein Serine-Threonine Kinases*/genetics ; Protein Serine-Threonine Kinases*/metabolism ; Cytokinesis* ; Mitosis*; Cell Cycle Proteins/genetics ; Cell Cycle Proteins/metabolism ; Humans ; Cell Cycle |
| Abstract: | Subcellular location and activation of Tank Binding Kinase 1 (TBK1) govern precise progression through mitosis. Either loss of activated TBK1 or its sequestration from the centrosomes causes errors in mitosis and growth defects. Yet, what regulates its recruitment and activation on the centrosomes is unknown. We identified that NAK-associated protein 1 (NAP1) is essential for mitosis, binding to and activating TBK1, which both localize to centrosomes. Loss of NAP1 causes several mitotic and cytokinetic defects due to inactivation of TBK1. Our quantitative phosphoproteomics identified numerous TBK1 substrates that are not only confined to the centrosomes but are also associated with microtubules. Substrate motifs analysis indicates that TBK1 acts upstream of other essential cell cycle kinases like Aurora and PAK kinases. We also identified NAP1 as a TBK1 substrate phosphorylating NAP1 at S318 to promote its degradation by the ubiquitin proteasomal system. These data uncover an important distinct function for the NAP1-TBK1 complex during cell division.; (This is a work of the U.S. Government and is not subject to copyright protection in the United States. Foreign copyrights may apply.) |
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| Grant Information: | P30 CA008748 United States CA NCI NIH HHS; R35 CA197588 United States CA NCI NIH HHS; R35 GM142368 United States GM NIGMS NIH HHS; GM142368 United States NH NIH HHS |
| Substance Nomenclature: | 0 (AZI2 protein, human); 0 (Cell Cycle Proteins); EC 2.7.11.1 (TBK1 protein, human); 0 (Adaptor Proteins, Signal Transducing); EC 2.7.11.1 (Protein Serine-Threonine Kinases) |
| Entry Date(s): | Date Created: 20231207 Date Completed: 20231218 Latest Revision: 20241116 |
| Update Code: | 20260130 |
| PubMed Central ID: | PMC10702366 |
| DOI: | 10.1083/jcb.202303082 |
| PMID: | 38059900 |
| Database: | MEDLINE |
Journal Article; Research Support, N.I.H., Extramural