Analysis of IL-1β, TGF-β, IL-5, ACE, PTPN22 gene polymorphisms, and gene expression levels in Turkish children with IgA vasculitis.
| Title: | Analysis of IL-1β, TGF-β, IL-5, ACE, PTPN22 gene polymorphisms, and gene expression levels in Turkish children with IgA vasculitis. |
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| Authors: | Taşkın RB; Faculty of Medicine, Department of Pediatric Rheumatology, Ege University, Izmir, Turkey. rzyburcuguven@hotmail.com.; Aydın İ; Faculty of Medicine, Department of Pediatric Rheumatology, Ege University, Izmir, Turkey.; Aytaç G; Faculty of Medicine, Department of Pediatric Rheumatology, Ege University, Izmir, Turkey.; Imamoglu S; Faculty of Medicine, Department of Pediatric Rheumatology, Ege University, Izmir, Turkey.; Tunçay SC; Faculty of Medicine, Department of Pediatric Nephrology, Ege University, Izmir, Turkey.; Bulut İK; Faculty of Medicine, Department of Pediatric Nephrology, Ege University, Izmir, Turkey.; Karaca NE; Faculty of Medicine, Department of Pediatrics, Division of Pediatric Immunology, Ege University, Kazimdirik Neighborhood, University Street Number: 9, 35100, Bornova, Izmir, Turkey.; Aksu G; Faculty of Medicine, Department of Pediatric Rheumatology, Ege University, Izmir, Turkey.; Faculty of Medicine, Department of Pediatrics, Division of Pediatric Immunology, Ege University, Kazimdirik Neighborhood, University Street Number: 9, 35100, Bornova, Izmir, Turkey.; Berdeli A; Faculty of Medicine, Department of Pediatric Rheumatology, Ege University, Izmir, Turkey.; Kütükçüler N; Faculty of Medicine, Department of Pediatric Rheumatology, Ege University, Izmir, Turkey.; Faculty of Medicine, Department of Pediatrics, Division of Pediatric Immunology, Ege University, Kazimdirik Neighborhood, University Street Number: 9, 35100, Bornova, Izmir, Turkey. |
| Source: | Molecular biology reports [Mol Biol Rep] 2023 Dec 12; Vol. 51 (1), pp. 15. Date of Electronic Publication: 2023 Dec 12. |
| Publication Type: | Journal Article |
| Language: | English |
| Journal Info: | Publisher: Reidel Country of Publication: Netherlands NLM ID: 0403234 Publication Model: Electronic Cited Medium: Internet ISSN: 1573-4978 (Electronic) Linking ISSN: 03014851 NLM ISO Abbreviation: Mol Biol Rep Subsets: MEDLINE |
| Imprint Name(s): | Original Publication: Dordrecht, Boston, Reidel. |
| MeSH Terms: | IgA Vasculitis*/genetics ; Arthritis*; Interleukin-5/genetics ; Transforming Growth Factor beta/genetics ; Peptidyl-Dipeptidase A/genetics ; Polymorphism, Genetic/genetics ; RNA, Messenger/genetics ; Gene Frequency/genetics ; Polymorphism, Single Nucleotide/genetics ; Child ; Humans ; Genotype ; Real-Time Polymerase Chain Reaction ; Gene Expression ; Genetic Predisposition to Disease ; Case-Control Studies ; Protein Tyrosine Phosphatase, Non-Receptor Type 22 |
| Abstract: | Objective: Immunoglobulin-A vasculitis (IgAV) is an inflammatory disease that affects small blood vessels. This study was performed to identify an association between protein tyrosine phosphatase non-receptor type 22 (PTPN22) + 788G > A (rs33996649), transforming growth factor-beta (TGF-β) -509C > T (rs18004069), interleukin 1-beta (IL-1β) -511C > T (rs16944), interleukin 5 (IL-5) -746C/T (rs2069812), and angiotensin-converting enzyme (ACE) I/D (rs4646994) gene polymorphisms, susceptibility to IgAV, as well as the mRNA levels of IL-1β, IL-1β, and TGF-β.; Method: A total of 53 patients with IgAV and 50 healthy controls were enrolled. PTPN22, TGF-β, IL-1β, ACE gene polymorphisms, ACE gene I/D polymorphisms, and mRNA expression levels were analyzed using the polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method, allele-specific PCR, and real-time PCR with TaqMan kits, respectively.; Results: PTPN22, TGF-β, IL-1β, IL-5, and ACE variants showed no genotype or allele differences between patients with IgAV and controls. Increased levels of IL-1β and TGF-β mRNA expressions were observed in patients with IgAV (p |
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| Contributed Indexing: | Keywords: ACE gene polymorphism; IgA vasculitis; Proinflammatory cytokine gene polymorphisms |
| Substance Nomenclature: | 0 (Interleukin-5); 0 (Transforming Growth Factor beta); EC 3.4.15.1 (Peptidyl-Dipeptidase A); 0 (RNA, Messenger); EC 3.1.3.48 (PTPN22 protein, human); EC 3.1.3.48 (Protein Tyrosine Phosphatase, Non-Receptor Type 22) |
| Entry Date(s): | Date Created: 20231212 Date Completed: 20231216 Latest Revision: 20260127 |
| Update Code: | 20260130 |
| DOI: | 10.1007/s11033-023-08944-x |
| PMID: | 38085361 |
| Database: | MEDLINE |
Journal Article