Distinguishing host responses, extensive viral dissemination and long-term viral RNA persistence in domestic sheep experimentally infected with Crimean-Congo haemorrhagic fever virus Kosovo Hoti.
| Title: | Distinguishing host responses, extensive viral dissemination and long-term viral RNA persistence in domestic sheep experimentally infected with Crimean-Congo haemorrhagic fever virus Kosovo Hoti. |
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| Authors: | Li H; National Centre for Foreign Animal Disease, Canadian Food Inspection Agency, Winnipeg, Canada.; Pinette M; National Centre for Foreign Animal Disease, Canadian Food Inspection Agency, Winnipeg, Canada.; Smith G; National Centre for Foreign Animal Disease, Canadian Food Inspection Agency, Winnipeg, Canada.; Goolia M; National Centre for Foreign Animal Disease, Canadian Food Inspection Agency, Winnipeg, Canada.; Handel K; National Centre for Foreign Animal Disease, Canadian Food Inspection Agency, Winnipeg, Canada.; Nebroski M; National Centre for Foreign Animal Disease, Canadian Food Inspection Agency, Winnipeg, Canada.; Lung O; National Centre for Foreign Animal Disease, Canadian Food Inspection Agency, Winnipeg, Canada.; Pickering BS; National Centre for Foreign Animal Disease, Canadian Food Inspection Agency, Winnipeg, Canada.; Department of Medical Microbiology and Infectious Diseases, College of Medicine, Faculty of Health Sciences, University of Manitoba, Winnipeg, Canada. |
| Source: | Emerging microbes & infections [Emerg Microbes Infect] 2024 Dec; Vol. 13 (1), pp. 2302103. Date of Electronic Publication: 2024 Jan 22. |
| Publication Type: | Journal Article |
| Language: | English |
| Journal Info: | Publisher: Taylor & Francis Country of Publication: United States NLM ID: 101594885 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 2222-1751 (Electronic) Linking ISSN: 22221751 NLM ISO Abbreviation: Emerg Microbes Infect Subsets: MEDLINE |
| Imprint Name(s): | Publication: 2019- : [Philadelphia, PA] : Taylor & Francis; Original Publication: New York : NPG, 2012-2018. |
| MeSH Terms: | Hemorrhagic Fever, Crimean*/diagnosis ; Hemorrhagic Fever Virus, Crimean-Congo* ; Ticks*; Sheep, Domestic/genetics ; RNA, Viral/genetics ; Humans ; Animals ; Mice ; Sheep ; Kosovo ; Interleukin-8 |
| Abstract: | Crimean-Congo haemorrhagic fever orthonairovirus (CCHFV) is a tick-borne, risk group 4 pathogen that often causes a severe haemorrhagic disease in humans (CCHF) with high case fatality rates. The virus is believed to be maintained in a tick-vertebrate-tick ecological cycle involving numerous wild and domestic animal species; however the biology of CCHFV infection in these animals remains poorly understood. Here, we experimentally infect domestic sheep with CCHFV Kosovo Hoti, a clinical isolate representing high pathogenicity to humans and increasingly utilized in current research. In the absence of prominent clinical signs, the infection leads to an acute viremia and coinciding viral shedding, fever and markers for potential impairment in liver and kidney functions. A number of host responses distinguish the subclinical infection in sheep versus fatal infection in humans. These include an early reduction of neutrophil recruitment and its chemoattractant, IL-8, in the blood stream of infected sheep, whereas neutrophil infiltration and elevated IL-8 are features of fatal CCHFV infections reported in immunodeficient mice and humans. Several inflammatory cytokines that correlate with poor disease outcomes in humans and have potential to cause vascular dysfunction, a primary hallmark of severe CCHF, are down-regulated or restricted from increasing in sheep. Of particular interest, the detection of CCHFV RNA (including full-length genome) in a variety of sheep tissues long after the acute phase of infection indicates a widespread viral dissemination in the host and suggests a potentially long-term persisting impact of CCHFV infection. These findings reveal previously unrecognized aspects of CCHFV biology in animals. |
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| Contributed Indexing: | Keywords: Crimean-Congo haemorrhagic fever virus; Kosovo Hoti; RNA persistence; sheep; virus shedding |
| Substance Nomenclature: | 0 (RNA, Viral); 0 (Interleukin-8) |
| Entry Date(s): | Date Created: 20240108 Date Completed: 20240124 Latest Revision: 20241023 |
| Update Code: | 20260130 |
| PubMed Central ID: | PMC10810640 |
| DOI: | 10.1080/22221751.2024.2302103 |
| PMID: | 38189080 |
| Database: | MEDLINE |
Journal Article