Selective REM sleep restriction in mice using a device designed for tunable somatosensory stimulation.
| Title: | Selective REM sleep restriction in mice using a device designed for tunable somatosensory stimulation. |
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| Authors: | Huffman DM; F. Joseph Halcomb, III M.D. Department of Biomedical Engineering, University of Kentucky, Lexington, KY, USA.; Ajwad AA; F. Joseph Halcomb, III M.D. Department of Biomedical Engineering, University of Kentucky, Lexington, KY, USA.; Agarwal A; Signal Solutions, LLC, Lexington, KY, USA.; Lhamon ME; Signal Solutions, LLC, Lexington, KY, USA.; Donohue K; Signal Solutions, LLC, Lexington, KY, USA.; O'Hara BF; Signal Solutions, LLC, Lexington, KY, USA.; Sunderam S; F. Joseph Halcomb, III M.D. Department of Biomedical Engineering, University of Kentucky, Lexington, KY, USA. Electronic address: ssu223@uky.edu. |
| Source: | Journal of neuroscience methods [J Neurosci Methods] 2024 Apr; Vol. 404, pp. 110063. Date of Electronic Publication: 2024 Jan 30. |
| Publication Type: | Journal Article; Research Support, N.I.H., Extramural |
| Language: | English |
| Journal Info: | Publisher: Elsevier/North-Holland Biomedical Press Country of Publication: Netherlands NLM ID: 7905558 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1872-678X (Electronic) Linking ISSN: 01650270 NLM ISO Abbreviation: J Neurosci Methods Subsets: MEDLINE |
| Imprint Name(s): | Original Publication: Amsterdam, Elsevier/North-Holland Biomedical Press. |
| MeSH Terms: | Sleep, REM*/physiology ; Sleep, Slow-Wave*; Sleep/physiology ; Mice ; Animals ; Arousal ; Homeostasis ; Electroencephalography |
| Abstract: | Background: Sleep perturbation is widely used to investigate the physiological mechanisms that mediate sleep-wake dynamics, and to isolate the specific roles of sleep in health and disease. However, state-of-the-art methods to accomplish sleep perturbation in preclinical models are limited in their throughput, flexibility, and specificity.; New Method: A system was developed to deliver vibro-tactile somatosensory stimulation aimed at controlled, selective sleep perturbation. The frequency and intensity of stimulation can be tuned to target a variety of experimental applications, from sudden arousal to sub-threshold transitions between light and deep stages of NREM sleep. This device was activated in closed-loop to selectively interrupt REM sleep in mice.; Results: Vibro-tactile stimulation effectively and selectively interrupted REM sleep - significantly reducing the average REM bout duration relative to matched, unstimulated baseline recordings. As REM sleep was repeatedly interrupted, homeostatic mechanisms prompted a progressively quicker return to REM sleep. These effects were dependent on the parameters of stimulation applied.; Comparison With Existing Methods: Existing sleep perturbation systems often require moving parts within the cage and/or restrictive housing. The system presented is unique in that it interrupts sleep without invading the animal's space. The ability to vary stimulation parameters is a great advantage over existing methods, as it allows for adaptation in response to habituation and/or circadian/homeostatic changes in arousal threshold.; Conclusions: The proposed method of stimulation demonstrates feasibility in affecting mouse sleep within a standard home cage environment, thus limiting environmental stress. Furthermore, the ability to tune frequency and intensity of stimulation allows for graded control over the extent of sleep perturbation, which potentially expands the utility of this technology beyond applications related to sleep.; (Copyright © 2024 Elsevier B.V. All rights reserved.) |
| Competing Interests: | Declaration of Competing Interest ML, KD, and BO are co-owners of Signal Solutions, LLC, which has since commercialized the device described in this study. Since completing this research, DH has become an employee of Signal Solutions, LLC. Other authors have no conflicts of interest to disclose. |
| References: | Mol Neurobiol. 2018 Apr;55(4):2884-2896. (PMID: 28455701); Pharmacol Biochem Behav. 2013 Aug;109:8-15. (PMID: 23603033); Sleep. 1998 Mar 15;21(2):138-42. (PMID: 9542797); Am J Physiol. 1991 Jul;261(1 Pt 2):R198-208. (PMID: 1858947); J Sleep Res. 2016 Apr;25(2):131-43. (PMID: 26762182); Am J Physiol Regul Integr Comp Physiol. 2015 Jun 15;308(12):R1062-9. (PMID: 25876653); Annu Int Conf IEEE Eng Med Biol Soc. 2016 Aug;2016:1640-1643. (PMID: 28268644); Elife. 2021 Oct 06;10:. (PMID: 34612204); Int J Neural Syst. 2016 Jun;26(4):1650017. (PMID: 27121993); Curr Biol. 2019 Feb 4;29(3):392-401.e4. (PMID: 30686738); J Am Assoc Lab Anim Sci. 2007 Jan;46(1):52-7. (PMID: 17203917); Neuroimage. 2017 Sep;158:70-78. (PMID: 28676297); Sleep. 2020 Jul 13;43(7):. (PMID: 31927578); Neural Regen Res. 2020 Jun;15(6):1094-1101. (PMID: 31823890); J Sleep Res. 2021 Aug;30(4):e13262. (PMID: 33403714); Annu Int Conf IEEE Eng Med Biol Soc. 2014;2014:3771-4. (PMID: 25570812); J Neurosci Methods. 2015 Aug 15;251:83-91. (PMID: 26014662); Behav Brain Res. 2018 Feb 26;339:124-129. (PMID: 29180134); Neuropharmacology. 2016 Sep;108:415-25. (PMID: 27150557); Neuroscience. 2019 May 15;406:22-37. (PMID: 30826520); J Sleep Res. 2015 Jun;24(3):309-19. (PMID: 25524602); Front Syst Neurosci. 2013 Dec 13;7:106. (PMID: 24379759); Brain Res. 2013 Sep 5;1529:200-8. (PMID: 23856323); Sleep Med Rev. 2015 Feb;19:29-38. (PMID: 24846773); Neurotherapeutics. 2019 Jul;16(3):554-568. (PMID: 31376067); Neuroreport. 2013 Jan 23;24(2):91-5. (PMID: 23238166); Brain Stimul. 2009 Oct;2(4):201-7, 207.e1. (PMID: 20648973); PLoS One. 2014 Sep 16;9(9):e107544. (PMID: 25226519); J Vis Exp. 2018 Oct 11;(140):. (PMID: 30371669); Comp Med. 2014 Feb;64(1):13-24. (PMID: 24512957); Sleep. 2000 Mar 15;23(2):175-83. (PMID: 10737334); J Sleep Res. 2022 Dec;31(6):e13603. (PMID: 35665551) |
| Grant Information: | R43 NS083218 United States NS NINDS NIH HHS; R44 NS083218 United States NS NINDS NIH HHS |
| Contributed Indexing: | Keywords: Automated sleep perturbation; Mouse; REM sleep restriction; Real-time sleep classification; Somatosensory stimulation |
| Entry Date(s): | Date Created: 20240201 Date Completed: 20240227 Latest Revision: 20250402 |
| Update Code: | 20260130 |
| PubMed Central ID: | PMC10922658 |
| DOI: | 10.1016/j.jneumeth.2024.110063 |
| PMID: | 38301833 |
| Database: | MEDLINE |
Journal Article; Research Support, N.I.H., Extramural