High biocompatible FITC-conjugated silica nanoparticles for cell labeling in both in vitro and in vivo models.
| Title: | High biocompatible FITC-conjugated silica nanoparticles for cell labeling in both in vitro and in vivo models. |
|---|---|
| Authors: | Nguyen TT; Center for Quantum and Electronics, Institute of Physics, Vietnam Academy of Science and Technology, 18 Hoang Quoc Viet Street, Hanoi, Vietnam.; Nguyen HN; Nano and Energy Center, VNU University of Science, Hanoi, 334 Nguyen Trai Street, Thanh Xuan, Hanoi, Vietnam.; Nghiem THL; Center for Quantum and Electronics, Institute of Physics, Vietnam Academy of Science and Technology, 18 Hoang Quoc Viet Street, Hanoi, Vietnam.; Do XH; Department of Practical and Experimental Surgery, Vietnam Military Medical University, 160 Phung Hung Street, Phuc La, Ha Dong, Hanoi, Vietnam.; To TT; Faculty of Biology, VNU University of Science, Hanoi, 334 Nguyen Trai Street, Thanh Xuan, Hanoi, 10000, Vietnam.; Do TXP; Faculty of Biology, VNU University of Science, Hanoi, 334 Nguyen Trai Street, Thanh Xuan, Hanoi, 10000, Vietnam.; Do DL; Faculty of Biology, VNU University of Science, Hanoi, 334 Nguyen Trai Street, Thanh Xuan, Hanoi, 10000, Vietnam.; Nguyen HG; Faculty of Biology, VNU University of Science, Hanoi, 334 Nguyen Trai Street, Thanh Xuan, Hanoi, 10000, Vietnam.; Nguyen HM; Faculty of Biology, VNU University of Science, Hanoi, 334 Nguyen Trai Street, Thanh Xuan, Hanoi, 10000, Vietnam.; Nguyen ND; Faculty of Physics, VNU University of Science, Hanoi, 334 Nguyen Trai Street, Thanh Xuan, Hanoi, Vietnam.; Luu MQ; Faculty of Physics, VNU University of Science, Hanoi, 334 Nguyen Trai Street, Thanh Xuan, Hanoi, Vietnam.; Nguyen TN; Center for Quantum and Electronics, Institute of Physics, Vietnam Academy of Science and Technology, 18 Hoang Quoc Viet Street, Hanoi, Vietnam.; Nguyen TBN; Center for Quantum and Electronics, Institute of Physics, Vietnam Academy of Science and Technology, 18 Hoang Quoc Viet Street, Hanoi, Vietnam.; Nguyen VT; Center for Quantum and Electronics, Institute of Physics, Vietnam Academy of Science and Technology, 18 Hoang Quoc Viet Street, Hanoi, Vietnam.; Pham VT; Faculty of Physics, VNU University of Science, Hanoi, 334 Nguyen Trai Street, Thanh Xuan, Hanoi, Vietnam.; Than UTT; Vinmec Hi-Tech Center and Vinmec-VinUni Institute of Immunology, Vinmec Healthcare System, 458 Minh Khai Street, Hanoi, Vietnam.; Hoang TMN; Faculty of Biology, VNU University of Science, Hanoi, 334 Nguyen Trai Street, Thanh Xuan, Hanoi, 10000, Vietnam. hoangthimynhung@hus.edu.vn. |
| Source: | Scientific reports [Sci Rep] 2024 Mar 23; Vol. 14 (1), pp. 6969. Date of Electronic Publication: 2024 Mar 23. |
| Publication Type: | Journal Article |
| Language: | English |
| Journal Info: | Publisher: Nature Publishing Group Country of Publication: England NLM ID: 101563288 Publication Model: Electronic Cited Medium: Internet ISSN: 2045-2322 (Electronic) Linking ISSN: 20452322 NLM ISO Abbreviation: Sci Rep Subsets: MEDLINE |
| Imprint Name(s): | Original Publication: London : Nature Publishing Group, copyright 2011- |
| MeSH Terms: | Silicon Dioxide*/chemistry ; Nanoparticles*/toxicity ; Nanoparticles*/chemistry; Animals ; Humans ; HeLa Cells ; Fluorescein-5-isothiocyanate ; Endothelial Cells |
| Abstract: | Fluorescence nanosilica-based cell tracker has been explored and applied in cell biological research. However, the aggregation of these nanoparticles at physiological pH is still the main limitation. In this research, we introduced a novel fluorescence nano-based cell tracker suitable for application in live cells. The silica-coated fluorescein isothiocyanate isomer (FITC-SiO2) nanoparticles (NPs) were modified with carboxymethylsilanetriol disodium salt (FITC-SiO2-COOH), integrating the dianion form of FITC molecules. This nanosystem exhibited superior dispersion in aqueous solutions and effectively mitigated dye leakage. These labeled NPs displayed notable biocompatibility and minimal cytotoxicity in both in vitro and in vivo conditions. Significantly, the NPs did not have negative implications on cell migration or angiogenesis. They successfully penetrated primary fibroblasts, human umbilical vein endothelial cells and HeLa cells in both 2D and 3D cultures, with the fluorescence signal enduring for over 72 h. Furthermore, the NP signals were consistently observed in the developing gastrointestinal tract of live medaka fish larvae for extended periods during phases of subdued digestive activity, without manifesting any apparent acute toxicity. These results underscore the promising utility of FITC-SiO2-COOH NPs as advanced live cell trackers in biological research.; (© 2024. The Author(s).) |
| Comments: | Erratum in: Sci Rep. 2025 Jul 11;15(1):25104. doi: 10.1038/s41598-025-10067-1.. (PMID: 40646104) |
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| Grant Information: | VINIF.2020.DA07 Quỹ Đổi mới sáng tạo Vingroup |
| Contributed Indexing: | Keywords: Biocompatibility; Cell labelling; Fluorescein isothiocyanate; Silica nanoparticles |
| Substance Nomenclature: | I223NX31W9 (Fluorescein-5-isothiocyanate); 7631-86-9 (Silicon Dioxide) |
| Entry Date(s): | Date Created: 20240324 Date Completed: 20240325 Latest Revision: 20250713 |
| Update Code: | 20260130 |
| PubMed Central ID: | PMC10960792 |
| DOI: | 10.1038/s41598-024-55600-w |
| PMID: | 38521815 |
| Database: | MEDLINE |
Journal Article