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Postmortem mitochondria function in longissimus lumborum of Angus and Brahman steers.

Title: Postmortem mitochondria function in longissimus lumborum of Angus and Brahman steers.
Authors: Ramos PM; Department of Animal Sciences, University of Florida, Gainesville, FL 32611, United States of America.; Wohlgemuth SE; Department of Physiology and Aging, University of Florida, Gainesville, FL 32603, United States of America.; Gingerich CA; Department of Animal Sciences, University of Florida, Gainesville, FL 32611, United States of America.; Hawryluk B; Department of Animal Sciences, University of Florida, Gainesville, FL 32611, United States of America.; Smith MT; Department of Animal Sciences, University of Florida, Gainesville, FL 32611, United States of America.; Bell LC; Department of Animal Sciences, University of Florida, Gainesville, FL 32611, United States of America.; Scheffler TL; Department of Animal Sciences, University of Florida, Gainesville, FL 32611, United States of America. Electronic address: tscheffler@ufl.edu.
Source: Meat science [Meat Sci] 2024 Sep; Vol. 215, pp. 109538. Date of Electronic Publication: 2024 May 11.
Publication Type: Journal Article
Language: English
Journal Info: Publisher: Elsevier Country of Publication: England NLM ID: 101160862 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1873-4138 (Electronic) Linking ISSN: 03091740 NLM ISO Abbreviation: Meat Sci Subsets: MEDLINE
Imprint Name(s): Publication: - : Oxford : Elsevier; Original Publication: Barking, Essex, Eng., Applied Science Publishers
MeSH Terms: Muscle, Skeletal*/chemistry ; Muscle, Skeletal*/metabolism ; Adenosine Triphosphate*/metabolism ; Red Meat*/analysis ; Glycolysis* ; Postmortem Changes* ; Oxidative Phosphorylation*; Mitochondria/metabolism ; Mitochondria, Muscle/metabolism ; Animals ; Cattle ; Male ; Hydrogen-Ion Concentration ; Oxygen Consumption ; Temperature
Abstract: Mitochondria function and integrity may impact postmortem metabolism and meat quality development. Adaptations in heat tolerant Brahman may persist to limit cellular stress postmortem. Our objective was to evaluate glycolysis, pH decline, and mitochondria function in longissimus lumborum (LL) from Angus and Brahman steers (N = 28) early postmortem (1 to 6 h) and after rigor (24 h). We evaluated metabolites of anaerobic glycolysis, ATP, pH, and temperature, and determined mitochondria oxygen consumption rate (OCR) in permeabilized fibers. The main effects of breed (b) and time (t) and the interaction were tested. Brahman LL contained greater ATP during the first 6 h postmortem; Brahman also tended to exhibit a slower pH decline (b × t, P = 0.07) and more rapid temperature decline (b × t, P 
Competing Interests: Declaration of competing interest The authors declare that there are no financial or personal relationships with other people or organizations that could inappropriately influence their work.
Contributed Indexing: Keywords: Bos indicus; Mitochondria; Postmortem metabolism; Respiration; pH decline
Substance Nomenclature: 8L70Q75FXE (Adenosine Triphosphate)
Entry Date(s): Date Created: 20240521 Date Completed: 20240613 Latest Revision: 20240613
Update Code: 20260130
DOI: 10.1016/j.meatsci.2024.109538
PMID: 38772311
Database: MEDLINE

Journal Article