Biomimetic laser-induced graphene fern leaf and enzymatic biosensor for pesticide spray collection and monitoring.
| Title: | Biomimetic laser-induced graphene fern leaf and enzymatic biosensor for pesticide spray collection and monitoring. |
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| Authors: | Jared NM; Department of Mechanical Engineering, Iowa State University, Ames, Iowa 50011, USA. jcclauss@iastate.edu.; Johnson ZT; Department of Mechanical Engineering, Iowa State University, Ames, Iowa 50011, USA. jcclauss@iastate.edu.; Pola CC; Department of Mechanical Engineering, Iowa State University, Ames, Iowa 50011, USA. jcclauss@iastate.edu.; Bez KK; Department of Mechanical Engineering, Iowa State University, Ames, Iowa 50011, USA. jcclauss@iastate.edu.; Bez K; Department of Chemistry, Iowa State University, Ames, Iowa 50011, USA.; Hooe SL; Center for Bio/Molecular Science and Engineering, Code 6900, Naval Research Laboratory, Washington, DC 20375, USA.; Breger JC; Center for Bio/Molecular Science and Engineering, Code 6900, Naval Research Laboratory, Washington, DC 20375, USA.; Smith EA; Department of Chemistry, Iowa State University, Ames, Iowa 50011, USA.; Medintz IL; Center for Bio/Molecular Science and Engineering, Code 6900, Naval Research Laboratory, Washington, DC 20375, USA.; Neihart NM; Department of Electrical Engineering, Iowa State University, Ames, Iowa 50011, USA.; Claussen JC; Department of Mechanical Engineering, Iowa State University, Ames, Iowa 50011, USA. jcclauss@iastate.edu. |
| Source: | Nanoscale horizons [Nanoscale Horiz] 2024 Aug 19; Vol. 9 (9), pp. 1543-1556. Date of Electronic Publication: 2024 Aug 19. |
| Publication Type: | Journal Article; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S. |
| Language: | English |
| Journal Info: | Publisher: Royal Society of Chemistry Country of Publication: England NLM ID: 101712576 Publication Model: Electronic Cited Medium: Internet ISSN: 2055-6764 (Electronic) Linking ISSN: 20556756 NLM ISO Abbreviation: Nanoscale Horiz Subsets: MEDLINE |
| Imprint Name(s): | Original Publication: [Cambridge, England] : Royal Society of Chemistry, [2016]- |
| MeSH Terms: | Graphite*/chemistry ; Biosensing Techniques*/methods ; Biosensing Techniques*/instrumentation ; Plant Leaves*/chemistry ; Pesticides*/analysis ; Pesticides*/chemistry ; Ferns*/chemistry ; Platinum*/chemistry ; Lasers*; Glycine/chemistry ; Glycine/analogs & derivatives ; Metal Nanoparticles/chemistry ; Biomimetics/methods ; Biomimetic Materials/chemistry ; Glyphosate |
| Abstract: | Monitoring of pesticide concentration distribution across farm fields is crucial to ensure precise and efficient application while preventing overuse or untreated areas. Inspired by nature's wettability patterns, we developed a biomimetic fern leaf pesticide collection patch using laser-induced graphene (LIG) alongside an external electrochemical LIG biosensor. This "collect-and-sense" system allows for rapid pesticide spray monitoring in the farm field. The LIG is synthesized and patterned on polyimide through a high-throughput gantry-based CO2 laser process, making it amenable to scalable manufacturing. The resulting LIG-based leaf exhibits a remarkable water collection capacity, harvesting spray mist/fog at a rate approximately 11 times greater than a natural ostrich fern leaf when the collection is normalized to surface area. The developed three-electrode LIG pesticide biosensor, featuring a working electrode functionalized with electrodeposited platinum nanoparticles (PtNPs) and the enzyme glycine oxidase, displayed a linear range of 10-260 μM, a detection limit of 1.15 μM, and a sensitivity of 5.64 nA μM-1 for the widely used herbicide glyphosate. Also, a portable potentiostat with a user-friendly interface was developed for remote operation, achieving an accuracy of up to 97%, when compared to a standard commercial benchtop potentiostat. The LIG "collect-and-sense" system can consistently collect and monitor glyphosate spray after 24-48 hours of spraying, a time that corresponds to the restricted-entry interval required to enter most farm fields after pesticide spraying. Hence, this innovative "collect-and-sense" system not only advances precision agriculture by enabling monitoring and mapping of pesticide distribution but also holds the potential to significantly reduce environmental impact, enhance crop management practices, and contribute to the sustainable and efficient use of agrochemicals in modern agriculture. |
| Grant Information: | CMMI-2037026 National Science Foundation; 2021-67021-34457 USDA; 2021-67011-35130 USDA; 2020-67021- 2020-67021 USDA; Office of Naval Research |
| Substance Nomenclature: | 7782-42-5 (Graphite); 0 (Pesticides); 49DFR088MY (Platinum); TE7660XO1C (Glycine); 4632WW1X5A (Glyphosate) |
| Entry Date(s): | Date Created: 20240710 Date Completed: 20240819 Latest Revision: 20241107 |
| Update Code: | 20260130 |
| DOI: | 10.1039/d4nh00010b |
| PMID: | 38985448 |
| Database: | MEDLINE |
Journal Article; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S.