Optimization of donor cell production for somatic cell nuclear transfer in the critically endangered Vietnamese Ỉ pig.
| Title: | Optimization of donor cell production for somatic cell nuclear transfer in the critically endangered Vietnamese Ỉ pig. |
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| Authors: | Nguyen VK; Key Lab of Animal Cell Technology, National Institute of Animal Science, Hanoi, Viet Nam. Electronic address: cotihin@gmail.com.; Thu Huong VT; Key Lab of Animal Cell Technology, National Institute of Animal Science, Hanoi, Viet Nam.; Le Thi Nguyen H; Key Lab of Animal Cell Technology, National Institute of Animal Science, Hanoi, Viet Nam.; Quan HX; Key Lab of Animal Cell Technology, National Institute of Animal Science, Hanoi, Viet Nam.; Hoang AT; Key Lab of Animal Cell Technology, National Institute of Animal Science, Hanoi, Viet Nam.; Phan HT; Key Lab of Animal Cell Technology, National Institute of Animal Science, Hanoi, Viet Nam.; Van Le Đ; Key Lab of Animal Cell Technology, National Institute of Animal Science, Hanoi, Viet Nam.; Nguyen HT; Key Lab of Animal Cell Technology, National Institute of Animal Science, Hanoi, Viet Nam.; Thi Nguyen NT; Key Lab of Animal Cell Technology, National Institute of Animal Science, Hanoi, Viet Nam.; Thi Nguyen AL; Key Lab of Animal Cell Technology, National Institute of Animal Science, Hanoi, Viet Nam.; Thi Pham YK; Key Lab of Animal Cell Technology, National Institute of Animal Science, Hanoi, Viet Nam.; Hung LP; Key Lab of Animal Cell Technology, National Institute of Animal Science, Hanoi, Viet Nam.; Thanh Nhan GT; Key Lab of Animal Cell Technology, National Institute of Animal Science, Hanoi, Viet Nam.; Trinh SH; Thuy Phuong Pig Research and Development Centre, National Institute of Animal Science, Hanoi, Viet Nam.; Pham LD; Key Lab of Animal Cell Technology, National Institute of Animal Science, Hanoi, Viet Nam. |
| Source: | Theriogenology [Theriogenology] 2024 Dec; Vol. 230, pp. 115-120. Date of Electronic Publication: 2024 Sep 14. |
| Publication Type: | Journal Article |
| Language: | English |
| Journal Info: | Publisher: Elsevier Country of Publication: United States NLM ID: 0421510 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1879-3231 (Electronic) Linking ISSN: 0093691X NLM ISO Abbreviation: Theriogenology Subsets: MEDLINE |
| Imprint Name(s): | Publication: [New York, N.Y.?] : Elsevier; Original Publication: Los Altos, Calif., Geron-X. |
| MeSH Terms: | Nuclear Transfer Techniques*/veterinary ; Endangered Species*; Blastocyst/physiology ; Cloning, Organism/veterinary ; Cloning, Organism/methods ; Embryo Culture Techniques/veterinary ; Embryo Transfer/veterinary ; Oocytes/physiology ; Swine/embryology ; Animals ; Female ; Male ; Pregnancy ; Embryonic Development ; Vietnam |
| Abstract: | We aimed to establish efficient donor cells to produce piglets by somatic cell nuclear transfer (SCNT) of the endangered Vietnamese Ỉ pig. In Experiment 1, we assessed the effects of cell passages on the in vitro development of SCNT embryos. Cells with five and six passages showed significantly cleaved and blastocyst formation rates (86.72 and 86.64; 35.68 and 35.51, respectively, P 0.05). Experiment 3 was performed to assess the effect of cell lines on the development of SCNT embryos. Our results showed no significant difference in the success rate of fibroblast nuclear transfer into recipient oocytes, the cleaved and blastocyst formation rates, and the average total cell number per blastocyst among the cell lines 6004, 9154, 9155, 9156 and 9157 (P > 0.05). Experiment 4 was performed to assess the ability of SCNT embryos to induce pregnancy and to develop term. SCNT embryos were produced from Ỉ fibroblast cells established based on the results of Experiments 1, 2 and 3. Transfer of blastocyst stage embryos into 19 recipients (100-120 embryos in each) resulted in 14 pregnancies, in which 8 pregnant females terminated on Day 22-42 and 6 others produced 20 cloned piglets from donor cells of a female pig but 5 piglets died before birth and 15 healthy cloned piglets. However, 3 out of 15 healthy piglets died of unknown causes within 24h of birth and 3 out of 15 healthy piglets died at 3-5 days of age due to diarrhoea, 9 out of 15 healthy piglets are now 3 months of age. Finally, we established a protocol for the donor cell production which enabled the production of the endangered Ỉ pig embryos by SCNT and maximized blastocyst production rate by more than 35 % and pregnant rate after the transfer of cloned Ỉ pig embryos to recipients at 73.68 % for the first time in Vietnam.; (Copyright © 2024 Elsevier Inc. All rights reserved.) |
| Contributed Indexing: | Keywords: Cell lines; Donor cells passage; Embryo transfer; Somatic cell nuclear transfer; The sex of donor cells; Vietnamese Ỉ pig |
| Entry Date(s): | Date Created: 20240918 Date Completed: 20241017 Latest Revision: 20241021 |
| Update Code: | 20260130 |
| DOI: | 10.1016/j.theriogenology.2024.09.013 |
| PMID: | 39293173 |
| Database: | MEDLINE |
Journal Article