Impact of semen cryopreservation season on in vitro embryo production of prepubertal goat oocytes.
| Title: | Impact of semen cryopreservation season on in vitro embryo production of prepubertal goat oocytes. |
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| Authors: | Ferrer-Roda M; Department of Animal and Food Science, Veterinary Faculty, Autonomous University of Barcelona, Barcelona, Spain.; Catalán J; Biotechnology of Animal and Human Reproduction (TechnoSperm), Institute of Food and Agricultural Technology, University of Girona, Girona, Spain.; Unit of Cell Biology, Department of Biology, Faculty of Sciences, University of Girona, Girona, Spain.; Department of Animal Medicine and Surgery, Faculty of Veterinary Sciences, Autonomous University of Barcelona, Barcelona, Spain.; Izquierdo D; Department of Animal and Food Science, Veterinary Faculty, Autonomous University of Barcelona, Barcelona, Spain.; Oliveira MEF; Department of Animal and Food Science, Veterinary Faculty, Autonomous University of Barcelona, Barcelona, Spain.; School of Agricultural and Veterinarian Sciences, São Paulo State University, Sao Paulo, Brazil.; Paramio MT; Department of Animal and Food Science, Veterinary Faculty, Autonomous University of Barcelona, Barcelona, Spain. |
| Source: | Reproduction in domestic animals = Zuchthygiene [Reprod Domest Anim] 2024 Oct; Vol. 59 Suppl 3, pp. e14594. |
| Publication Type: | Journal Article |
| Language: | English |
| Journal Info: | Publisher: Paul Parey Scientific Publishers Country of Publication: Germany NLM ID: 9015668 Publication Model: Print Cited Medium: Internet ISSN: 1439-0531 (Electronic) Linking ISSN: 09366768 NLM ISO Abbreviation: Reprod Domest Anim Subsets: MEDLINE |
| Imprint Name(s): | Original Publication: Berlin ; Hamburg : Paul Parey Scientific Publishers, c1990- |
| MeSH Terms: | Goats*/physiology ; Cryopreservation*/veterinary ; Oocytes*/physiology ; Fertilization in Vitro*/veterinary ; Seasons*; Semen Preservation/veterinary ; Semen Preservation/methods ; Spermatozoa/physiology ; In Vitro Oocyte Maturation Techniques/veterinary ; Sexual Maturation/physiology ; Embryo Culture Techniques/veterinary ; Calcium/metabolism ; Calcium/analysis ; Semen Analysis/veterinary ; Animals ; Male ; Female ; Sperm Capacitation ; Membrane Potential, Mitochondrial |
| Abstract: | Goat production is affected by reproductive seasonality. In vitro embryo production (IVEP) could overcome this effect. This study aimed to evaluate the impact of the season of semen collection/freezing on IVEP of prepubertal goat oocytes and on sperm quality and functionality concerning capacitation. Semen from six fertile bucks was collected, pooled and cryopreserved in spring and autumn and used for IVEP of oocytes recovered during the breeding season. Oocytes were IVM in TCM-199 with hormones, EGF and cysteamine; fertilized and cultured in BO-IVF and BO-IVC media (IVF Bioscience, UK). Semen samples were assessed at 0 and 3 h after culture in capacitating (BO-IVF, CAP) and non-capacitating conditions for sperm plasma membrane and acrosome integrity, mitochondrial membrane potential (MMP), intracellular calcium and plasma membrane lipid disorder. Blastocyst production was higher with spring sperm compared to autumn (12.0% vs. 2.1%, respectively; p |
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| Grant Information: | Ministerio de Ciencia, Innovación y Universidades; Generalitat de Catalunya |
| Contributed Indexing: | Keywords: IVEP; IVF; goat; sperm capacitation |
| Substance Nomenclature: | SY7Q814VUP (Calcium) |
| Entry Date(s): | Date Created: 20241013 Date Completed: 20241013 Latest Revision: 20241013 |
| Update Code: | 20260130 |
| DOI: | 10.1111/rda.14594 |
| PMID: | 39396865 |
| Database: | MEDLINE |
Journal Article