Effect of biphasic in vitro maturation (CAPA-IVM) on EGF receptor and embryo development of prepubertal goat oocytes according to follicle size.
| Title: | Effect of biphasic in vitro maturation (CAPA-IVM) on EGF receptor and embryo development of prepubertal goat oocytes according to follicle size. |
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| Authors: | Ferrer-Roda M; Department of Animal and Food Science, Autonomous University of Barcelona, Barcelona, Spain.; Gil A; Department of Animal and Food Science, Autonomous University of Barcelona, Barcelona, Spain.; Paramio MT; Department of Animal and Food Science, Autonomous University of Barcelona, Barcelona, Spain.; Izquierdo D; Department of Animal and Food Science, Autonomous University of Barcelona, Barcelona, Spain. |
| Source: | Animal biotechnology [Anim Biotechnol] 2024 Nov; Vol. 35 (1), pp. 2422316. Date of Electronic Publication: 2024 Nov 10. |
| Publication Type: | Journal Article |
| Language: | English |
| Journal Info: | Publisher: Taylor & Francis Country of Publication: England NLM ID: 9011409 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1532-2378 (Electronic) Linking ISSN: 10495398 NLM ISO Abbreviation: Anim Biotechnol Subsets: MEDLINE |
| Imprint Name(s): | Publication: 2005- : Abingdon, Oxford : Taylor & Francis; Original Publication: New York, N.Y. : Marcel Dekker, c1990- |
| MeSH Terms: | ErbB Receptors*/metabolism ; Oocytes*/physiology ; Oocytes*/drug effects ; In Vitro Oocyte Maturation Techniques*/veterinary ; Ovarian Follicle*/physiology ; Ovarian Follicle*/drug effects ; Embryonic Development*/physiology ; Goats*; Natriuretic Peptide, C-Type/pharmacology ; Reactive Oxygen Species/metabolism ; Blastocyst/physiology ; Meiosis/physiology ; Animals ; Female |
| Abstract: | Oocytes spontaneously resume meiosis following their liberation from follicles, preventing full competence acquisition. Biphasic IVM (CAPA-IVM) maintains oocytes in meiotic arrest to improve developmental competence, and it specially affects poorly developed oocytes. We assessed the effect of CAPA-IVM on oocytes from small (3mm) follicles of prepubertal goats. Oocytes were cultured for 6h in pre-IVM with C-type natriuretic peptide (CNP) and estradiol as meiotic inhibitors, and germinal vesicle (GV) rate and chromatin configuration were assessed. Then, oocytes were cultured in conventional IVM (c-IVM) or CAPA-IVM (pre-IVM + c-IVM) and EGF receptor (EGFR) protein expression, intra-oocyte ROS and blastocyst development were assessed. GV rate was higher in CNP groups than control (69% vs 28%, and 67% vs 31%, small and large follicles, respectively), but GV chromatin configuration was similar. In large follicles, EGFR expression was higher in oocytes and cumulus cells after CAPA-IVM, and ROS levels were lower. In small follicles these differences were not observed. c-IVM and CAPA-IVM produced similar blastocyst rates in small (3.7% vs 2.6%, respectively) and large follicles (8.3% vs 2.5%). Overall, CAPA-IVM enhanced EGFR expression for EGF peptide signalling and antioxidant capacity in oocytes from large follicles but oocytes from small follicles were too immature to benefit from it. |
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| Contributed Indexing: | Keywords: EGFR; GV chromatin configuration; blastocyst production; epidermal growth factor; follicle diameter; oocyte competence |
| Substance Nomenclature: | EC 2.7.10.1 (ErbB Receptors); 127869-51-6 (Natriuretic Peptide, C-Type); 0 (Reactive Oxygen Species) |
| Entry Date(s): | Date Created: 20241110 Date Completed: 20241110 Latest Revision: 20251211 |
| Update Code: | 20260130 |
| PubMed Central ID: | PMC12674236 |
| DOI: | 10.1080/10495398.2024.2422316 |
| PMID: | 39522054 |
| Database: | MEDLINE |
Journal Article