New Epitopes for the Serodiagnosis of Human Borreliosis.
| Title: | New Epitopes for the Serodiagnosis of Human Borreliosis. |
|---|---|
| Authors: | Alcón-Chino MET; Center for Technological Development in Health (CDTS), National Institute of Science and Technology for Innovation in Neglected Population Diseases (INCT-IDPN), FIOCRUZ, Rio de Janeiro 21040-900, RJ, Brazil.; Post-Graduation Program in Science and Biotechnology, Department of Molecular and Cellular Biology, Biology Institute, Federal Fluminense University, Niterói 22040-036, RJ, Brazil.; Bonoldi VLN; Clinical Hospital, Faculty of Medicine, São Paulo University, São Paulo 05403-000, SP, Brazil.; Pereira RMR; Faculty of Medicine, São Paulo University, São Paulo 01246-903, SP, Brazil.; Gazeta GS; Laboratory of Ticks and Other Wingless Arthropods-National Reference for Vectors of Rickettsioses, Instituto Oswaldo Cruz-IOC, FIOCRUZ, Rio de Janeiro 21041-250, RJ, Brazil.; Carvalho JPRS; Center for Technological Development in Health (CDTS), National Institute of Science and Technology for Innovation in Neglected Population Diseases (INCT-IDPN), FIOCRUZ, Rio de Janeiro 21040-900, RJ, Brazil.; Post-Graduation Program in Science and Biotechnology, Department of Molecular and Cellular Biology, Biology Institute, Federal Fluminense University, Niterói 22040-036, RJ, Brazil.; Napoleão-Pêgo P; Center for Technological Development in Health (CDTS), National Institute of Science and Technology for Innovation in Neglected Population Diseases (INCT-IDPN), FIOCRUZ, Rio de Janeiro 21040-900, RJ, Brazil.; Durans AM; Center for Technological Development in Health (CDTS), National Institute of Science and Technology for Innovation in Neglected Population Diseases (INCT-IDPN), FIOCRUZ, Rio de Janeiro 21040-900, RJ, Brazil.; Souza ALA; Multidisciplinary Biochemistry Teaching Laboratory, UNIG, Nova Iguaçu 26260-045, RJ, Brazil.; De-Simone SG; Center for Technological Development in Health (CDTS), National Institute of Science and Technology for Innovation in Neglected Population Diseases (INCT-IDPN), FIOCRUZ, Rio de Janeiro 21040-900, RJ, Brazil.; Post-Graduation Program in Science and Biotechnology, Department of Molecular and Cellular Biology, Biology Institute, Federal Fluminense University, Niterói 22040-036, RJ, Brazil.; Laboratory of Epidemiology and Molecular Systematics, Oswaldo Cruz Institut, FIOCRUZ, Rio de Janeiro 21040-900, RJ, Brazil. |
| Source: | Microorganisms [Microorganisms] 2024 Oct 31; Vol. 12 (11). Date of Electronic Publication: 2024 Oct 31. |
| Publication Type: | Journal Article |
| Language: | English |
| Journal Info: | Publisher: MDPI AG Country of Publication: Switzerland NLM ID: 101625893 Publication Model: Electronic Cited Medium: Print ISSN: 2076-2607 (Print) Linking ISSN: 20762607 NLM ISO Abbreviation: Microorganisms Subsets: PubMed not MEDLINE |
| Imprint Name(s): | Original Publication: Basel, Switzerland : MDPI AG, [2013]- |
| Abstract: | Lyme disease, a zoonotic infection caused by the bacterium Borrelia burgdorferi, is transmitted to humans through the bites of infected ticks. Its diagnosis primarily relies on serological methods; however, the existing borreliosis techniques have shown a variable sensitivity and specificity. Our study aimed to map IgG epitopes from five outer membrane proteins (Omp) from B. burgdorferi [Filament flagellar 41kD (PI1089), flagellar hook-associated protein (Q44767), Flagellar hook k2 protein (O51173), Putative Omp BURGA03 (Q44849), and 31 kDa OspA (P0CL66)] lipoprotein to find specific epitopes for the development of accurate diagnosis methods. Using the spot synthesis technique, a library of 380 peptides was constructed to identify linear B cell epitopes recognized by human IgG in response to specific B. burgdorferi-associated proteins. The reactivity of this epitope when chemically synthesized was then evaluated using ELISA with a panel of the patient's sera. Cross-reactivity was assessed through data bank access and in vitro analysis. Among the 19 epitopes identified, four were selected for further investigation based on their signal intensity, secondary structure, and peptide matching. Validation was performed using ELISA, and ROC curve analysis demonstrated a sensitivity of ≥85.71%, specificity of ≥92.31, accuracy of ≥90.7, and AUC value of ≥0.91 for all peptides. Our cross-reactivity analysis demonstrated that the Burg/02/huG, Burg/03/huG, and Burg/12/huG peptides were not reactive to antibodies from patients with Leptospirosis and syphilis compared to those from the B. burgdorferi group. These peptides indicated an excellent performance in distinguishing between B. burgdorferi-infected and non-infected individuals and exhibited a neglected reactivity to antibodies in sera from patients with Leptospirosis and syphilis. These peptides are promising targets for recombinant development, potentially leading to more accurate serological tests and vaccines. |
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| Grant Information: | #010.101.029/2018 Carlos Chagas Filho Foundation of Research Support of the State of Rio de Janeiro (FAPERJ) |
| Contributed Indexing: | Keywords: Brazilian borreliosis; ELISA; cross-reactivity; epitope mapping |
| Entry Date(s): | Date Created: 20241127 Latest Revision: 20241130 |
| Update Code: | 20260130 |
| PubMed Central ID: | PMC11596413 |
| DOI: | 10.3390/microorganisms12112212 |
| PMID: | 39597601 |
| Database: | MEDLINE |
Journal Article