Effect of BMP15 and GDF9 in the IVM medium on subsequent oocyte competence and embryo development of prepubertal goats.
| Title: | Effect of BMP15 and GDF9 in the IVM medium on subsequent oocyte competence and embryo development of prepubertal goats. |
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| Authors: | Ferrer-Roda M; Department of Animal and Food Science, Veterinary Faculty, Autonomous University of Barcelona, 08193, Barcelona, Spain.; Paramio MT; Department of Animal and Food Science, Veterinary Faculty, Autonomous University of Barcelona, 08193, Barcelona, Spain.; Vila-Beltrán J; Department of Animal and Food Science, Veterinary Faculty, Autonomous University of Barcelona, 08193, Barcelona, Spain.; Izquierdo D; Department of Animal and Food Science, Veterinary Faculty, Autonomous University of Barcelona, 08193, Barcelona, Spain. Electronic address: dolors.izquierdo@uab.cat. |
| Source: | Theriogenology [Theriogenology] 2025 Mar 01; Vol. 234, pp. 164-173. Date of Electronic Publication: 2024 Dec 18. |
| Publication Type: | Journal Article |
| Language: | English |
| Journal Info: | Publisher: Elsevier Country of Publication: United States NLM ID: 0421510 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1879-3231 (Electronic) Linking ISSN: 0093691X NLM ISO Abbreviation: Theriogenology Subsets: MEDLINE |
| Imprint Name(s): | Publication: [New York, N.Y.?] : Elsevier; Original Publication: Los Altos, Calif., Geron-X. |
| MeSH Terms: | Growth Differentiation Factor 9*/pharmacology ; Growth Differentiation Factor 9*/genetics ; Bone Morphogenetic Protein 15*/pharmacology ; Goats*/physiology ; Oocytes*/drug effects ; In Vitro Oocyte Maturation Techniques*/veterinary ; In Vitro Oocyte Maturation Techniques*/methods ; Embryonic Development*/drug effects; Culture Media/pharmacology ; Embryo Culture Techniques/veterinary ; Sexual Maturation/drug effects ; Parthenogenesis/drug effects ; Cumulus Cells/drug effects ; Animals ; Female |
| Abstract: | Oocyte-secreted factors (OSFs), such as BMP15 and GDF9, are soluble paracrine factors that drive cumulus cell differentiation and function, sustaining oocyte competence acquisition and embryo development. This study aimed to assess the effect of BMP15 and GDF9 on IVM medium of prepubertal goat oocytes. COCs were in vitro matured in absence (control group) or presence of 100 ng/mL of BMP15, GDF9, or both. To determine cumulus-oocyte communication, transzonal projections (TZP) density at 0h, 6h, 12h and 24h of IVM were evaluated. After IVM, mitochondrial activity, intracellular ROS and glutathione (GSH) levels, the epidermal growth factor receptor (EGFR) expression in oocytes and cumulus cells, and cumulus expansion were assessed. Blastocyst production and quality were evaluated after parthenogenetic activation (PA) and IVF. IVM supplementation with BMP15 increased the TZP density during the first 6 h of culture. After IVM, BMP15 increased mitochondrial activity, EGFR expression in oocytes and cumulus cells, and cumulus expansion compared to control, but ROS and GSH levels were similar to control. BMP15 improved blastocyst production following PA (15.5 % vs 6.3 %) and the number of cells in the blastocyst inner cell mass. No differences were observed on blastocyst production or quality following IVF. IVM supplementation with GDF9 did not improve results from control group in any parameters studied. Additionally, GDF9 in combination with BMP15 only improved mitochondrial activity and cumulus expansion over control. In conclusion, IVM medium supplementation with BMP15 (100 ng/ml) improves COCs quality parameters and PA-blastocyst production and quality of prepubertal goat oocytes. However, GDF9 (100 ng/mL) did not have any beneficial effect in this study and was possibly antagonistic to BMP15.; (Copyright © 2024 The Authors. Published by Elsevier Inc. All rights reserved.) |
| Competing Interests: | Declaration of competing interest The authors declare that they have no competing interests. |
| Contributed Indexing: | Keywords: Blastocyst production; Cumulus-oocyte communication; Epidermal growth factor receptor; Mitochondrial activity; Oocyte secreted factors; Transzonal projections |
| Substance Nomenclature: | 0 (Growth Differentiation Factor 9); 0 (Bone Morphogenetic Protein 15); 0 (Culture Media) |
| Entry Date(s): | Date Created: 20241222 Date Completed: 20250109 Latest Revision: 20250109 |
| Update Code: | 20260130 |
| DOI: | 10.1016/j.theriogenology.2024.12.015 |
| PMID: | 39709802 |
| Database: | MEDLINE |
Journal Article