Assessing reagents and techniques for identifying RhCE variants in routine serological testing.
| Title: | Assessing reagents and techniques for identifying RhCE variants in routine serological testing. |
|---|---|
| Authors: | de Paula Vendrame TA; Colsan - Associação Beneficente de Coleta de Sangue, São Paulo, São Paulo, Brazil.; Arnoni CP; Colsan - Associação Beneficente de Coleta de Sangue, São Paulo, São Paulo, Brazil.; Devides GF; Colsan - Associação Beneficente de Coleta de Sangue, São Paulo, São Paulo, Brazil.; Silva NM; Colsan - Associação Beneficente de Coleta de Sangue, São Paulo, São Paulo, Brazil.; Cortez AJP; Colsan - Associação Beneficente de Coleta de Sangue, São Paulo, São Paulo, Brazil.; Roche Moreira Latini F; Colsan - Associação Beneficente de Coleta de Sangue, São Paulo, São Paulo, Brazil.; Castilho L; Hemocentro Unicamp, Campinas, São Paulo, Brazil. |
| Source: | Vox sanguinis [Vox Sang] 2025 Mar; Vol. 120 (3), pp. 301-309. Date of Electronic Publication: 2025 Jan 07. |
| Publication Type: | Journal Article |
| Language: | English |
| Journal Info: | Publisher: Blackwell Science Country of Publication: England NLM ID: 0413606 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1423-0410 (Electronic) Linking ISSN: 00429007 NLM ISO Abbreviation: Vox Sang Subsets: MEDLINE |
| Imprint Name(s): | Publication: 2001- : Oxford, UK : Blackwell Science; Original Publication: Basel : Karger |
| MeSH Terms: | Rh-Hr Blood-Group System*/genetics ; Rh-Hr Blood-Group System*/immunology ; Serologic Tests*/methods ; Blood Grouping and Crossmatching*/methods; Humans ; Blood Donors ; Indicators and Reagents |
| Abstract: | Background and Objectives: Identifying RhCE variants is essential to prevent alloimmunization and manage complex cases. Unfortunately, these variants are often only detected after antibody formation, as they may go unnoticed in serological tests. This study aimed to assess monoclonal antisera using various methodologies to define the reactivity patterns of some variants by variable expression of RhCE antigens.; Materials and Methods: Samples were chosen based on atypical reactivity on routine RhCE typing of donors, screening of Afro-descendant donors using tube testing and patient samples with antibodies against their own antigens. All 53 samples were tested using tube, gel and microplate tests with five antisera. Antigen expression was assessed by flow cytometry, and RhCE variants were molecularly classified.; Results: Tube test screening of African descent donors proved more effective in identifying a broad range of weak or partial antigens, particularly when using anti-e composed of MS-21, MS-16, MS63 clones and anti-c from the MS8011531019 clone. Automated instrument phenotyping successfully identified samples with RHCE*ceJAL allele, while most other variants were detected as positive (4+), similar to gel test, which intensified most reactions. When comparing methods and antisera for detecting variant e antigens, tube test identified a higher percentage of weak samples (63%-77%) compared with microplate (35%) and gel tests (14%).; Conclusion: The results highlight the critical role of tube test in serological routines and the need to select clones capable of identifying RhCE variants. Detecting reduced RhCE antigen expression during routine serological testing can guide further molecular investigations and help prevent Rh alloimmunization.; (© 2025 International Society of Blood Transfusion.) |
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| Grant Information: | Associação Beneficente de Coleta de Sangue (Colsan); International Society of Blood Transfusion (ISBT) |
| Contributed Indexing: | Keywords: RHCE genotyping; RhCE variants; serological testing |
| Substance Nomenclature: | 0 (Rh-Hr Blood-Group System); 0 (RHCE protein, human); 0 (Indicators and Reagents) |
| Entry Date(s): | Date Created: 20250108 Date Completed: 20250430 Latest Revision: 20250520 |
| Update Code: | 20260130 |
| DOI: | 10.1111/vox.13792 |
| PMID: | 39777635 |
| Database: | MEDLINE |
Journal Article