Stress-mediating inflammatory cytokine profiling reveals unique patterns in malaria and typhoid fever patients.
| Title: | Stress-mediating inflammatory cytokine profiling reveals unique patterns in malaria and typhoid fever patients. |
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| Authors: | Bin Eric M; Department of Biochemistry, University of Yaoundé 1, Yaoundé, Cameroon.; Molecular Diagnostics Research Group, Biotechnology Centre, University of Yaoundé 1, Yaoundé, Cameroon.; Laboratory for Public Health Research Biotechnologies, University of Yaoundé 1, Yaoundé, Cameroon.; Netongo PM; Department of Biochemistry, University of Yaoundé 1, Yaoundé, Cameroon.; Molecular Diagnostics Research Group, Biotechnology Centre, University of Yaoundé 1, Yaoundé, Cameroon.; Laboratory for Public Health Research Biotechnologies, University of Yaoundé 1, Yaoundé, Cameroon.; School of Science, Navajo Technical University, Crownpoint, New Mexico, United States of America.; Kamdem SD; Molecular Diagnostics Research Group, Biotechnology Centre, University of Yaoundé 1, Yaoundé, Cameroon.; Department of Pathology, University of Utah School of Medicine, Utah, Salt Lake City, United States of America.; Nzuno C; Molecular Diagnostics Research Group, Biotechnology Centre, University of Yaoundé 1, Yaoundé, Cameroon.; Department of Medical Laboratory Sciences, University of Buea, Buea, Cameroon.; Tchoutang AM; Department of Biochemistry, University of Yaoundé 1, Yaoundé, Cameroon.; Molecular Diagnostics Research Group, Biotechnology Centre, University of Yaoundé 1, Yaoundé, Cameroon.; Laboratory for Public Health Research Biotechnologies, University of Yaoundé 1, Yaoundé, Cameroon.; Berenger TKE; Molecular Diagnostics Research Group, Biotechnology Centre, University of Yaoundé 1, Yaoundé, Cameroon.; Faculty of Medicine and Biomedical Sciences, University of Yaoundé 1, Yaoundé, Cameroon.; Buri BD; Department of Biochemistry, University of Yaoundé 1, Yaoundé, Cameroon.; Molecular Diagnostics Research Group, Biotechnology Centre, University of Yaoundé 1, Yaoundé, Cameroon.; Ngum NL; Molecular Diagnostics Research Group, Biotechnology Centre, University of Yaoundé 1, Yaoundé, Cameroon.; Laboratory for Public Health Research Biotechnologies, University of Yaoundé 1, Yaoundé, Cameroon.; Faculty of Medicine and Biomedical Sciences, University of Yaoundé 1, Yaoundé, Cameroon.; Institute for Medical Research and Medicinal Plants Studies, Yaoundé, Cameroon.; Chedjou JP; Laboratory for Public Health Research Biotechnologies, University of Yaoundé 1, Yaoundé, Cameroon.; Department of Biochemistry, Faculty of Science, University of Buea, Buea, Cameroon.; Nji AM; Department of Biochemistry, University of Yaoundé 1, Yaoundé, Cameroon.; Laboratory for Public Health Research Biotechnologies, University of Yaoundé 1, Yaoundé, Cameroon.; Mbacham WF; Department of Biochemistry, University of Yaoundé 1, Yaoundé, Cameroon.; Laboratory for Public Health Research Biotechnologies, University of Yaoundé 1, Yaoundé, Cameroon. |
| Source: | PloS one [PLoS One] 2025 Feb 27; Vol. 20 (2), pp. e0306585. Date of Electronic Publication: 2025 Feb 27 (Print Publication: 2025). |
| Publication Type: | Journal Article; Observational Study |
| Language: | English |
| Journal Info: | Publisher: Public Library of Science Country of Publication: United States NLM ID: 101285081 Publication Model: eCollection Cited Medium: Internet ISSN: 1932-6203 (Electronic) Linking ISSN: 19326203 NLM ISO Abbreviation: PLoS One Subsets: MEDLINE |
| Imprint Name(s): | Original Publication: San Francisco, CA : Public Library of Science |
| MeSH Terms: | Typhoid Fever*/blood ; Typhoid Fever*/psychology ; Typhoid Fever*/immunology ; Typhoid Fever*/epidemiology ; Malaria*/blood ; Malaria*/immunology ; Malaria*/psychology ; Cytokines*/blood ; Stress, Psychological*/blood; Hydrocortisone/blood ; Inflammation/blood ; Humans ; Male ; Female ; Adult ; Cross-Sectional Studies ; Middle Aged ; Young Adult ; Adolescent |
| Abstract: | Malaria and typhoid fever pose significant health risks, leading to severe morbidity and mortality when inadequately treated. Understanding the role of stress-related inflammatory cytokines is crucial, as they mediate immune responses that affect pathogen clearance and recovery. This study investigated the cytokine profiles in patients with malaria and/or typhoid fever attending the Obala District Hospital in Yaoundé, Cameroon. We conducted a cross-sectional observational study measuring cortisol and inflammatory cytokines in blood samples from 55 infected patients and a control group of 15 healthy individuals using ELISA kits. We also evaluated psychological stress over the past 30 days using a 10-item Perceived Stress Scale (PSS) questionnaire to explore the link between stress and immune response. Psychological stress levels were notably higher in the typhoid fever group (18.20 ± 5.5) compared to the other groups, although these differences were statistically insignificant. Cortisol levels were significantly elevated (p |
| Competing Interests: | The authors have declared that no competing interests exist. |
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| Grant Information: | United Kingdom WT_ Wellcome Trust |
| Substance Nomenclature: | 0 (Cytokines); WI4X0X7BPJ (Hydrocortisone) |
| Entry Date(s): | Date Created: 20250227 Date Completed: 20250227 Latest Revision: 20250519 |
| Update Code: | 20260130 |
| PubMed Central ID: | PMC11867312 |
| DOI: | 10.1371/journal.pone.0306585 |
| PMID: | 40014608 |
| Database: | MEDLINE |
Journal Article; Observational Study