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Impact of microabrasion and a remineralizing agent before in-office bleaching on hydrogen peroxide permeability, color alteration, and enamel morphology.

Title: Impact of microabrasion and a remineralizing agent before in-office bleaching on hydrogen peroxide permeability, color alteration, and enamel morphology.
Authors: Favoreto MW; Department of Restorative Dentistry, State University of Ponta Grossa, Parana, Brazil; Department of Restorative Dentistry, Tuiuti University of Parana, Curitiba, Parana, Brazil. Electronic address: michaelfavoreto@hotmail.com.; Condolo L; Department of Restorative Dentistry, State University of Ponta Grossa, Parana, Brazil. Electronic address: leticiaccondolo@gmail.com.; Camargo CM; Department of Restorative Dentistry, State University of Ponta Grossa, Parana, Brazil. Electronic address: cami254631@gmail.com.; Lima RR; Laboratory of Functional and Structural Biology, Institute of Biological Sciences, Federal University of Pará, Belém, Brazil. Electronic address: rafalima@ufpa.br.; Carrillo K; Facultad de Ciencias de la Salud, Carrera de Odontologia, Universidad Hemisferios, Quito, Ecuador. Electronic address: kjcarrillor@profesores.uhemisferios.edu.ec.; Calixto AL; Department of Restorative Dentistry, State University of Ponta Grossa, Parana, Brazil. Electronic address: alcalixto@ig.com.br.; Reis A; Department of Restorative Dentistry, State University of Ponta Grossa, Parana, Brazil. Electronic address: reis_ale@hotmail.com.; Loguercio AD; Department of Restorative Dentistry, State University of Ponta Grossa, Parana, Brazil; Facultad de Ciencias de la Salud, Carrera de Odontologia, Universidad Hemisferios, Quito, Ecuador. Electronic address: aloguercio@hotmail.com.
Source: Journal of dentistry [J Dent] 2025 May; Vol. 156, pp. 105655. Date of Electronic Publication: 2025 Mar 01.
Publication Type: Journal Article
Language: English
Journal Info: Publisher: Elsevier Country of Publication: England NLM ID: 0354422 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1879-176X (Electronic) Linking ISSN: 03005712 NLM ISO Abbreviation: J Dent Subsets: MEDLINE
Imprint Name(s): Publication: Kidlington : Elsevier; Original Publication: Bristol, Eng., Wright.
MeSH Terms: Hydrogen Peroxide*/pharmacokinetics ; Hydrogen Peroxide*/pharmacology ; Dental Enamel*/drug effects ; Dental Enamel*/ultrastructure ; Tooth Bleaching*/methods ; Caseins*/pharmacology ; Tooth Bleaching Agents*/pharmacokinetics ; Tooth Bleaching Agents*/pharmacology ; Tooth Remineralization*/methods ; Enamel Microabrasion*/methods; Bicuspid/drug effects ; Dental Pulp Cavity/drug effects ; Dental Enamel Permeability/drug effects ; Humans ; Microscopy, Electron, Scanning ; Color ; Spectrophotometry
Abstract: Objective: This in vitro study evaluated hydrogen peroxide (HP) penetration into the pulp chamber, bleaching efficacy, and surface enamel morphology of human teeth subjected to enamel microabrasion (MA) combined with in-office bleaching (IOB), with or without the application of casein phosphopeptide-amorphous calcium phosphate (CPP-ACP) remineralizing agent.; Methods: Forty sound premolars were randomly divided into four groups: no treatment (NT), IOB only (IOB), MA followed by IOB (MA+IOB), and MA followed by CPP-ACP and then IOB (MA+CPP-ACP+IOB). HP concentration in the pulp chamber was measured using UV-Vis spectrophotometry, while bleaching efficacy was assessed with a digital spectrophotometer. Surface morphology was analyzed using scanning electron microscopy (SEM), and data were statistically analyzed.; Results: The NT group exhibited lower HP penetration compared to experimental groups, while MA+IOB showed higher HP levels than IOB and MA+CPP-ACP+IOB. All experimental groups demonstrated significant color changes, except NT. The MA+IOB group displayed more grooves on enamel surfaces than the MA+CPP-ACP+IOB group, which had remineralizing agent deposits.; Conclusion: Combining MA and IOB increases HP penetration into the pulp chamber, but using CPP-ACP after MA can mitigate this effect without compromising bleaching efficacy.; Clinical Significance: Employing a CPP-ACP remineralizing agent post-microabrasion can help reduce HP levels in the pulp chamber while maintaining effective whitening.; (Copyright © 2025. Published by Elsevier Ltd.)
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
Contributed Indexing: Keywords: Dental enamel permeability; Dentin permeability; Enamel microabrasion; Hydrogen Peroxide
Substance Nomenclature: BBX060AN9V (Hydrogen Peroxide); 0 (Caseins); 0 (Tooth Bleaching Agents); 0 (casein phosphopeptide-amorphous calcium phosphate nanocomplex)
Entry Date(s): Date Created: 20250302 Date Completed: 20250418 Latest Revision: 20250418
Update Code: 20260130
DOI: 10.1016/j.jdent.2025.105655
PMID: 40024516
Database: MEDLINE

Journal Article