Pangenome Analysis of the Plant Pathogen Pseudomonas syringae Reveals Unique Natural Products for Niche Adaptation.
| Title: | Pangenome Analysis of the Plant Pathogen Pseudomonas syringae Reveals Unique Natural Products for Niche Adaptation. |
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| Authors: | Zhang S; Department of Paleobiotechnology, Leibniz Institute for Natural Product Research and Infection Biology - Hans Knöll Institute, Beutenbergstraße 11a, D-07745, Jena, Germany.; Huang Y; Department of Paleobiotechnology, Leibniz Institute for Natural Product Research and Infection Biology - Hans Knöll Institute, Beutenbergstraße 11a, D-07745, Jena, Germany.; Nachawati R; Department of Paleobiotechnology, Leibniz Institute for Natural Product Research and Infection Biology - Hans Knöll Institute, Beutenbergstraße 11a, D-07745, Jena, Germany.; Huber P; Department of Paleobiotechnology, Leibniz Institute for Natural Product Research and Infection Biology - Hans Knöll Institute, Beutenbergstraße 11a, D-07745, Jena, Germany.; Walther G; National Reference Center for Invasive Fungal Infections, Leibniz Institute for Natural Product Research and Infection Biology - Hans Knöll Institute, Beutenbergstraße 11a, D-07745, Jena, Germany.; Gregor L; Institute of Organic Chemistry and Macromolecular Chemistry, Friedrich Schiller University Jena, Humboldtstraße 10, D-07743, Jena, Germany.; Vilotijević I; Institute of Organic Chemistry and Macromolecular Chemistry, Friedrich Schiller University Jena, Humboldtstraße 10, D-07743, Jena, Germany.; Cluster of Excellence Balance of the Microverse, Friedrich Schiller University Jena, Fürstengraben 1, D-07743, Jena, Germany.; Stallforth P; Department of Paleobiotechnology, Leibniz Institute for Natural Product Research and Infection Biology - Hans Knöll Institute, Beutenbergstraße 11a, D-07745, Jena, Germany.; Institute of Organic Chemistry and Macromolecular Chemistry, Friedrich Schiller University Jena, Humboldtstraße 10, D-07743, Jena, Germany.; Cluster of Excellence Balance of the Microverse, Friedrich Schiller University Jena, Fürstengraben 1, D-07743, Jena, Germany. |
| Source: | Angewandte Chemie (International ed. in English) [Angew Chem Int Ed Engl] 2025 Jun 17; Vol. 64 (25), pp. e202503679. Date of Electronic Publication: 2025 May 02. |
| Publication Type: | Journal Article |
| Language: | English |
| Journal Info: | Publisher: Wiley-VCH Country of Publication: Germany NLM ID: 0370543 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1521-3773 (Electronic) Linking ISSN: 14337851 NLM ISO Abbreviation: Angew Chem Int Ed Engl Subsets: MEDLINE |
| Imprint Name(s): | Publication: : Weinheim : Wiley-VCH; Original Publication: Weinheim/Bergstr. : New York, : Verlag Chemie ; Academic Press, c1962- |
| MeSH Terms: | Pseudomonas syringae*/genetics ; Pseudomonas syringae*/metabolism ; Pseudomonas syringae*/chemistry ; Biological Products*/chemistry ; Biological Products*/metabolism ; Biological Products*/pharmacology; Peptide Synthases/metabolism ; Peptide Synthases/genetics ; Multigene Family |
| Abstract: | Pseudomonas syringae is a soil-dwelling bacterium that exhibits remarkable niche adaptability, and it is known for its devastating impact as a plant pathogen. This bacterium has an outstanding capability to produce a wide array of biologically active natural products. P. syringae coexists with amoebal predators and fungal strains, which drives the production of secondary metabolites for predator evasion in addition to niche adaptation. In this study, we conducted a broad pangenomic analysis of 18 taxonomically distinct P. syringae strains, leading to the identification of 231 biosynthetic gene clusters (BGCs). Among these, nonribosomal peptide synthetases (NRPSs) were particularly abundant, indicating their potential significance within this ecological context. We discovered and elucidated the structures of two novel classes of bioactive compounds, the syrilipamides and chlorosecimides. Furthermore, a bioinformatic analysis enabled the identification of an undescribed halogenase, SecA, essential for the chlorination of secimide A. We observed that syrilipamides and secimides and in particular mixtures thereof, exhibit amoebicidal activities. Additionally, secimides showed selective antifungal activity. These findings provide valuable insights into the ecological roles of P. syringae natural products and highlight their potential for biotechnological and therapeutic applications.; (© 2025 The Author(s). Angewandte Chemie International Edition published by Wiley‐VCH GmbH.) |
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| Grant Information: | Werner Siemens Foundation; Leibniz Association; Deutsche Forschungsgemeinschaft |
| Contributed Indexing: | Keywords: Bioactivity; Halogenase; Natural products; P. syringae; Pangenome analysis |
| Substance Nomenclature: | 0 (Biological Products); EC 6.3.2.- (Peptide Synthases); EC 6.3.2.- (non-ribosomal peptide synthase) |
| Entry Date(s): | Date Created: 20250407 Date Completed: 20250617 Latest Revision: 20250619 |
| Update Code: | 20260130 |
| PubMed Central ID: | PMC12171316 |
| DOI: | 10.1002/anie.202503679 |
| PMID: | 40192321 |
| Database: | MEDLINE |
Journal Article