A mercury complex-based fluorescent sensor for biological thiols.
| Title: | A mercury complex-based fluorescent sensor for biological thiols. |
|---|---|
| Authors: | Hien NK; Mientrung Institute for Scientific Research, Vietnam National Museum of Nature, Vietnam Academy of Science and Technology Hue 49000 Vietnam.; Giao Chau TT; Mientrung Institute for Scientific Research, Vietnam National Museum of Nature, Vietnam Academy of Science and Technology Hue 49000 Vietnam.; Luyen ND; Department of Chemistry, Hue University Hue 49000 Vietnam duongtuanquang@dhsphue.edu.vn.; Vo QV; Faculty of Chemical Technology-Environment, The University of Danang - University of Technology and Education 48 Cao Thang Danang 50000 Vietnam.; Van Bay M; The University of Danang, University of Science and Education Danang 50000 Vietnam.; Ngo ST; Laboratory of Biophysics, Institute for Advanced Study in Technology, Ton Duc Thang University Ho Chi Minh City 72915 Vietnam.; Faculty of Pharmacy, Ton Duc Thang University Ho Chi Minh City 72915 Vietnam.; Nam PC; The University of Danang, University of Science and Technology Danang 50000 Vietnam pcnam@dut.udn.vn.; Quang DT; Department of Chemistry, Hue University Hue 49000 Vietnam duongtuanquang@dhsphue.edu.vn. |
| Source: | RSC advances [RSC Adv] 2025 Jun 13; Vol. 15 (25), pp. 20125-20133. Date of Electronic Publication: 2025 Jun 13 (Print Publication: 2025). |
| Publication Type: | Journal Article |
| Language: | English |
| Journal Info: | Publisher: Royal Society of Chemistry Country of Publication: England NLM ID: 101581657 Publication Model: eCollection Cited Medium: Internet ISSN: 2046-2069 (Electronic) Linking ISSN: 20462069 NLM ISO Abbreviation: RSC Adv Subsets: PubMed not MEDLINE |
| Imprint Name(s): | Original Publication: Cambridge [England] : Royal Society of Chemistry, [2011]- |
| Abstract: | A novel fluorescent sensor, Hg(DST)2, was developed for the selective detection of biological thiols, including glutathione (GSH), cysteine (Cys), and homocysteine (Hcy), in fully aqueous solutions at pH 7.2. The sensor exhibited significant fluorescence quenching upon coordination with Hg2+, which was reversibly restored in the presence of thiols due to the formation of thermodynamically favored Hg-thiol complexes. The OFF-ON fluorescence mechanism of the sensor was elucidated using DFT calculations. Fluorescence titration experiments revealed a strong linear correlation (R 2 ≈ 0.998) between fluorescence intensity and thiol concentrations within the ranges of 0.34-8.00 μM for GSH, 0.47-10.00 μM for Cys, and 0.26-8.00 μM for Hcy, with corresponding limits of detection (LOD) of 0.34, 0.47, and 0.26 μM, respectively. The sensor demonstrated high selectivity toward thiols in the presence of common amino acids, metal ions, and anions, with interference from Ag+, Cu2+, Co2+, and Ni2+ mitigated using 1,10-phenanthroline (PHEN). Owing to its high sensitivity, selectivity, and water solubility, Hg(DST)2 represents a promising tool for thiol quantification in biological and environmental matrices.; (This journal is © The Royal Society of Chemistry.) |
| Competing Interests: | There are no conflicts to declare. |
| References: | Nutr J. 2015 Jan 10;14:6. (PMID: 25577237); Annu Rev Nutr. 1999;19:217-46. (PMID: 10448523); JAMA. 1997 Jun 11;277(22):1775-81. (PMID: 9178790); Anal Chem. 2020 Mar 17;92(6):4177-4181. (PMID: 32126756); PLoS One. 2010 Sep 08;5(9):e12244. (PMID: 20838622); RSC Adv. 2018 Oct 19;8(62):35759-35767. (PMID: 35547895); Antioxidants (Basel). 2021 Feb 28;10(3):. (PMID: 33670839); J Chromatogr A. 2017 Nov 17;1524:29-36. (PMID: 28989034); Analyst. 2013 Jul 7;138(13):3802-8. (PMID: 23702918); Am J Pathol. 1969 Jul;56(1):111-28. (PMID: 5792556); Chem Soc Rev. 2013 Feb 7;42(3):845-56. (PMID: 23117144); J Am Chem Soc. 2017 Mar 1;139(8):3181-3185. (PMID: 28170238); Angew Chem Int Ed Engl. 2017 Oct 16;56(43):13188-13198. (PMID: 28703457); Biochemistry. 1964 Jun;3:745-50. (PMID: 14211608); Analyst. 2020 Apr 14;145(8):3029-3037. (PMID: 32134064); Chem Rev. 2005 Aug;105(8):2999-3093. (PMID: 16092826); RSC Adv. 2020 Oct 1;10(60):36265-36274. (PMID: 35517943); Crit Rev Anal Chem. 2022;52(3):649-666. (PMID: 32941060); RSC Adv. 2021 Jun 15;11(34):21116-21126. (PMID: 35479348); Sci Rep. 2021 Feb 16;11(1):3937. (PMID: 33594153); Talanta. 2000 Jun 30;52(3):509-15. (PMID: 18968010); Integr Med (Encinitas). 2014 Apr;13(2):8-15. (PMID: 26770084); Bioorg Chem. 2020 Jul;100:103923. (PMID: 32417525); Spectrochim Acta A Mol Biomol Spectrosc. 2020 Oct 5;239:118473. (PMID: 32447217); Biochem J. 1971 Feb;121(3):549-55. (PMID: 5119792); Sci Rep. 2019 Jan 14;9(1):115. (PMID: 30643157); ChemistryOpen. 2019 Oct 07;8(10):1251-1257. (PMID: 31608204); Front Cardiovasc Med. 2022 Jun 13;9:898305. (PMID: 35770226); Phys Rev B Condens Matter. 1988 Jan 15;37(2):785-789. (PMID: 9944570); Chem Res Toxicol. 1996 Jun;9(4):709-12. (PMID: 8831814); Clin Chem. 1993 Sep;39(9):1764-79. (PMID: 8375046); Chem Commun (Camb). 2020 Jun 25;56(51):7025-7028. (PMID: 32452476); Sensors (Basel). 2020 Feb 29;20(5):. (PMID: 32121408); Sensors (Basel). 2019 Jan 02;19(1):. (PMID: 30609736); BMJ. 2002 Nov 23;325(7374):1202. (PMID: 12446535); Anal Chem. 2022 May 10;94(18):6853-6859. (PMID: 35476395); Anal Bioanal Chem. 2020 Nov;412(28):7819-7826. (PMID: 32875370); Antioxidants (Basel). 2021 Jan 30;10(2):. (PMID: 33573222); Mol Aspects Med. 2009 Feb-Apr;30(1-2):29-41. (PMID: 18786561); Antioxidants (Basel). 2021 Jul 29;10(8):. (PMID: 34439468); J Chem Theory Comput. 2015 Nov 10;11(11):5340-59. (PMID: 26574326); Anal Chem. 2015 Mar 17;87(6):3308-13. (PMID: 25688569); Sensors (Basel). 2016 Jan 11;16(1):. (PMID: 26761012); Biochem J. 1953 Nov;55(4):649-52. (PMID: 13115349) |
| Entry Date(s): | Date Created: 20250616 Latest Revision: 20250617 |
| Update Code: | 20260130 |
| PubMed Central ID: | PMC12163708 |
| DOI: | 10.1039/d5ra02268a |
| PMID: | 40519683 |
| Database: | MEDLINE |
Journal Article