Cloning and Functional Characterization of a Novel Brevinin-1-Type Peptide from Sylvirana guentheri with Anticancer Activity.
| Title: | Cloning and Functional Characterization of a Novel Brevinin-1-Type Peptide from Sylvirana guentheri with Anticancer Activity. |
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| Authors: | La HT; Institute of Biology, Vietnam Academy of Science and Technology, Hanoi 100000, Vietnam.; Vietnam Academy of Science and Technology, Graduate University of Science and Technology, Hanoi 100000, Vietnam.; Nhu QBT; Faculty of Medical Technology, Hai Phong University of Medicine and Pharmacy, Hai Phong 180000, Vietnam.; Tran HM; Institute of Biology, Vietnam Academy of Science and Technology, Hanoi 100000, Vietnam.; Ngo HT; Institute of Biology, Vietnam Academy of Science and Technology, Hanoi 100000, Vietnam.; Le PMT; Institute of Biology, Vietnam Academy of Science and Technology, Hanoi 100000, Vietnam.; Hoang HH; Institute of Biology, Vietnam Academy of Science and Technology, Hanoi 100000, Vietnam.; Nguyen LT; Institute of Biology, Vietnam Academy of Science and Technology, Hanoi 100000, Vietnam.; Nguyen DT; Center for High Technology Research and Development, Vietnam Academy of Science and Technology, Hanoi 100000, Vietnam.; Ta TQ; Bac Thang Long Hospital, Hanoi 100000, Vietnam. |
| Source: | Current issues in molecular biology [Curr Issues Mol Biol] 2025 Aug 20; Vol. 47 (8). Date of Electronic Publication: 2025 Aug 20. |
| Publication Type: | Journal Article |
| Language: | English |
| Journal Info: | Publisher: MDPI Country of Publication: Switzerland NLM ID: 100931761 Publication Model: Electronic Cited Medium: Internet ISSN: 1467-3045 (Electronic) Linking ISSN: 14673037 NLM ISO Abbreviation: Curr Issues Mol Biol Subsets: PubMed not MEDLINE |
| Imprint Name(s): | Publication: 2021- : Basel, Switzerland : MDPI; Original Publication: Wymondham, Norfolk, UK : Caister Academic Press |
| Abstract: | Despite significant medical advancements, two major health challenges persist: antibiotic resistance in microbial pathogens and drug resistance in cancer cells. To address these issues, research has increasingly focused on discovering novel natural compounds with dual antimicrobial and anticancer activities. Among such candidates, antimicrobial peptides (AMPs) have attracted attention due to their ability to selectively target microbial and cancer cells while exhibiting minimal toxicity toward normal cells. Although Vietnam possesses rich biodiversity, including a wide range of Anura species, studies on AMPs from these organisms remain limited. In this study, a novel AMP, brevinin-1 E8.13, was identified from the skin secretion of Sylvirana guentheri, a frog species native to Vietnam. The brevinin-1 E8.13 peptide was successfully cloned, sequenced, and chemically synthesized. Functional assays revealed that brevinin-1 E8.13 possesses strong antibacterial activity against Staphylococcus aureus and exerts significant antiproliferative effects on various human cancer cell lines, including A549 (lung), AGS (gastric), Jurkat (leukemia), HCT116 (colorectal), HL60 (leukemia), and HepG2 (liver). The peptide demonstrated moderate to potent cytotoxic activity, with IC50 values ranging from 7.5 to 14.8 μM, depending on the cell type. Notably, brevinin-1 E8.13 exhibited low cytotoxicity toward normal human dermal fibroblast (HDF) cells and even promoted cell proliferation at lower concentrations. Furthermore, Chemically Activated Fluorescent Expression (CAFLUX) bioassay results confirmed that the peptide significantly downregulated Cyp1a1 gene expression in HepG2 cells. Collectively, these findings highlight the therapeutic potential of brevinin-1 E8.13 as a dual-function antimicrobial and anticancer agent derived from the skin secretion of Sylvirana guentheri. |
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| Grant Information: | TĐDIOX.04/22-24 Vietnam Academy of Science and Technology |
| Contributed Indexing: | Keywords: CAFLUX HepG2 cells; Sylvirana guentheri; anticancer activity; antimicrobial peptides; brevinin-1 E8.13; skin secretion |
| Entry Date(s): | Date Created: 20250827 Date Completed: 20250914 Latest Revision: 20250914 |
| Update Code: | 20260130 |
| PubMed Central ID: | PMC12412326 |
| DOI: | 10.3390/cimb47080673 |
| PMID: | 40864826 |
| Database: | MEDLINE |
Journal Article